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Invisibletekramrepus
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What can I do with 0.5cc of spores in a syringe
    #863680 - 09/04/02 03:02 PM (14 years, 3 months ago)

I have 0.5 cc left over of B+ in a syringe. What should I do with it?

Could I make honeywater? or what


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OfflineMechanicalMan
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Re: What can I do with 0.5cc of spores in a syringe [Re: tekramrepus]
    #863687 - 09/04/02 03:05 PM (14 years, 3 months ago)

Why don't you just make a PF jar?


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InvisibleRipple
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Re: What can I do with 0.5cc of spores in a syringe [Re: tekramrepus]
    #863690 - 09/04/02 03:08 PM (14 years, 3 months ago)

Make a jar with it it may colonize a bit slowly but it is certainly enough.


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Invisibletekramrepus
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Re: What can I do with 0.5cc of spores in a syringe [Re: Ripple]
    #863733 - 09/04/02 03:45 PM (14 years, 3 months ago)

So do I just do 2 holes on the sides, or one in the middle, or 4 like normal or what


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OfflineMechanicalMan
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Re: What can I do with 0.5cc of spores in a syringe [Re: tekramrepus]
    #863767 - 09/04/02 04:09 PM (14 years, 3 months ago)

3 or 4


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OfflineIchydai
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Re: What can I do with 0.5cc of spores in a syringe [Re: tekramrepus]
    #863896 - 09/04/02 05:33 PM (14 years, 3 months ago)

If I was you, I would try to use agar or something to grow it out, and then transfer it... I dont know, I really havent looked into the subject much, but thats what I would do. Or look at the spores under a microscope :wink:


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Offlinekillerjay
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Re: What can I do with 0.5cc of spores in a syringe [Re: tekramrepus]
    #863943 - 09/04/02 05:59 PM (14 years, 3 months ago)

If you want to get the most out of it and you don't have agar plates then I would reccommend honey water. As long as you are sure that the syringe isn't contaminated it could give you four or five syringes full of mycelium to inject many jars with. I use a half pint jar with a plastic lid that has one hole right at the edge so you can tip the jar and get at the water easily without it leaking out the other holes. I fill the jar half way with tap water add a few drops of honey ( I'm guessing about a half a teaspoon) and pressure cook at 15psi for an hour. If you don't contaminate it you should be ready to go in about a week and whatever jars you do with it should take off fast.


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OfflineIchydai
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Re: What can I do with 0.5cc of spores in a syringe [Re: killerjay]
    #863968 - 09/04/02 06:05 PM (14 years, 3 months ago)

Ive always wanted to do the mycelium honey-water thing... I think I'll try that tonight.


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InvisiblePinhead
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Re: What can I do with 0.5cc of spores in a syringe [Re: tekramrepus]
    #864104 - 09/04/02 07:16 PM (14 years, 3 months ago)

Just fill it up the rest of the way with cooled,sterile water..you'll never know the difference.


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InvisibleChampion des Champignons
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Re: What can I do with 0.5cc of spores in a syring [Re: Pinhead]
    #864596 - 09/05/02 02:16 AM (14 years, 3 months ago)

I've noticed that with a decently sporified syringe a lot of spores stick to the side of the syringe, and as the plunger pushes along the side it builds up a good amount of spores
Like pinhead said, if you suck up some fresh sterile water and give it a good shake you'll probably get another couple of good goes out of it.


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Offlinehappygrins
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Re: What can I do with 0.5cc of spores in a syringe [Re: killerjay]
    #864641 - 09/05/02 04:14 AM (14 years, 3 months ago)


>I fill the jar half way with tap water add a few drops of honey ( I'm guessing about a half a teaspoon) and pressure cook at 15psi for an hour.

PCing this for an hour isnt necessary.  This excessive time will cause it to carmelize thus adding a yellowish tint to it and making it harder to spot contams.  20 min. works great.

I also agree with pinhead...

--happygrins :grin:


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OfflineZed
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Re: What can I do with 0.5cc of spores in a syringe [Re: happygrins]
    #864697 - 09/05/02 05:09 AM (14 years, 3 months ago)

im not sure if i got away with this with no contams yet but i put some honey/water into a small tupperwear container with some seran wrap wrapped around the top of the container......then the top was put ontop of that. I then microwaved that for about 30seconds (Yes seconds!), by then it was boiling excessivly and the top had popped off slightly.

i injected with a syringe and white fluffy stuff started appearing everywhere..........how do u know if a honey/water is contam'd ???

i then made a little mistake, i sucked up 4 syringes with the honey/water because i was bored and had nothing to do..........5 days later and i looked at the syringe and it was THICK with growth, i had a very hard time getting the liquid out into the jars because the myc* kept blocking up the needle

ive got some jars with the honey/water incubating now. so time will tell if i got any contams.

Zed


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Invisiblefailbot999
Registered: 02/18/02
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Re: What can I do with 0.5cc of spores in a syringe *DELETED* [Re: Zed]
    #864736 - 09/05/02 05:39 AM (14 years, 3 months ago)

Post deleted by ski_stoned

Reason for deletion: delete



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Offlinekillerjay
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Registered: 10/26/01
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Re: What can I do with 0.5cc of spores in a syringe [Re: happygrins]
    #865875 - 09/05/02 05:58 PM (14 years, 3 months ago)

True an hour is too long for just honeywater jars. Actually I usually cook them longer though for about 1hour and 15 minutes, because I always just sterilize them while I'm doing my rye jars since I usually don't feel like firing up a 22qt cooker for just a couple small jars. So far I haven't had any problems with the mycelium growing in the water, but yes it does get a yellow tint do to carmalization.


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Offlinefrancisco
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Registered: 01/15/02
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Re: What can I do with 0.5cc of spores in a syringe [Re: failbot999]
    #866366 - 09/06/02 02:26 AM (14 years, 3 months ago)

Is that what killed the dog?


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Well...Maybe just a little.


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