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OfflineVisigoth
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Registered: 11/28/01
Posts: 481
Last seen: 11 years, 5 months
How much liquid mycelium to knock up a new jar??
    #828077 - 08/19/02 11:19 AM (21 years, 10 months ago)

In the tek for the liquid mycelium solution or honeywater, it says u can make more of the solution by injecting some of the liquid mycelium into a new jar. Anyone know how much u should use, 2 cc's of spores was used for the current jar. Also, it would be wise to suck up as much mycelium as possible for innocuation of the new jar right?? Any advice would be much appreciated. Thanks a ton!!!!

Vis


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"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

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Invisiblemickey_rourke
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Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: How much liquid mycelium to knock up a new jar?? [Re: Visigoth]
    #828081 - 08/19/02 11:21 AM (21 years, 10 months ago)

2 cc's should be fine here as well. I don't think there's a hard rule. My personal belief is that as long as some of the mycellium suspended in the liquid culture makes it to the new jar you will be just fine.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Offlinetchyted
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Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 9 years, 9 months
Re: How much liquid mycelium to knock up a new jar?? [Re: Visigoth]
    #828413 - 08/19/02 02:11 PM (21 years, 10 months ago)

as a general rule of thumb, you can use a syringe of licuid innoculant just like you would use spore-water and achieve similar results.
there is a limit to how many times you can create a new generation from a previous one in the manner you seem to suggest. my honey water gets contamned or goes weak after the second generation, so i have gotten to the point of not doing it. i use spores to make honey-water and use that to innoculate substrate or spawn, but not more honeywater.

the reason for this is economic. it is hard to tell good honeywater from slightly contamned. i can create a jar of honeywater for less than a dollar each, but throwing out a batch of spawn jars costs a whole lot more.

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OfflineChunkamunk
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Registered: 04/28/05
Posts: 4
Last seen: 7 years, 2 months
Re: How much liquid mycelium to knock up a new jar?? [Re: Visigoth]
    #4909653 - 11/08/05 03:30 PM (18 years, 8 months ago)

Watch that you dont use so much honey water that it messes with your ratio between brf, vermiculite, and water. I tried using the whole 10 cc's in a couple of my jars and then everything got real mushy and eventually was contaminated. Too much liquid in the jars.

Edited by Chunkamunk (11/08/05 03:31 PM)

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Offlineblackout
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Registered: 07/16/00 Happy 24th Shroomiversary!
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Re: How much liquid mycelium to knock up a new jar?? [Re: Chunkamunk]
    #4912419 - 11/09/05 03:18 AM (18 years, 8 months ago)

Leave the jars purposely dry if you are adding lots of LC

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OfflinekmIL
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Registered: 10/06/05
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Re: How much liquid mycelium to knock up a new jar?? [Re: blackout]
    #5028534 - 12/07/05 01:15 PM (18 years, 7 months ago)

What is the benefit of using liquid mycelium to knock up a jar rather than just a spore syringe? It seems just like an extra step to me.

(Unless the spores germinate in the liquid culture and speed up the colonization time, in which case I just answered my own question)

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Invisibleagar
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Registered: 11/21/04
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Re: How much liquid mycelium to knock up a new jar?? [Re: kmIL]
    #5028952 - 12/07/05 02:53 PM (18 years, 7 months ago)

2 ml of spore solution into an optimal LC will get you
500 to 1000 (++++++++++)ml of thickly colonized LC.

Spores need time to GERMINATE.

LC has already GERMINATED.

If you are careful not to have your grains to wet pre-inoculation.

You can inject 5 or even 10 ml of LC into a quart grain jar.

The result is more points of contact, and quicker colonization.

Moreover, a single 10 ml spore syringe (used frugally)
can propagate 2 gallons of well colonized liquid culture.

Which should/would last you for long while.



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OfflinekmIL
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Registered: 10/06/05
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Re: How much liquid mycelium to knock up a new jar?? [Re: agar]
    #5036423 - 12/08/05 09:43 PM (18 years, 7 months ago)

Where do you guys buy sterile syringes? I bought some spore syringes that (were at one point) obviously sterile, could I PC an old, empty one to sterilize it and then use it to inoculate some WBS with LC?

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Offlinewaixingren
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Registered: 03/14/05
Posts: 2,645
Loc: SW Florida
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Re: How much liquid mycelium to knock up a new jar?? [Re: kmIL]
    #5036474 - 12/08/05 09:50 PM (18 years, 7 months ago)

tractor supply! just went there today. they got an entire section. luer lock and slip tip. guages 16-22. 3cc-60cc. AND inoculation guns. not meant for mush cult but they gottem.


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