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OfflineErawk
Stranger
Registered: 11/04/01
Posts: 10
Loc: Vermont USA
Last seen: 22 years, 6 months
Liquid Mycelium Culturing
    #504791 - 12/30/01 12:45 PM (22 years, 6 months ago)

How many people do this? It seems like it would be way easier to keep a continuous fruiting setup going. You could incoculate your jars with mycilium instead of spores. Are there disadvantages to doing this? It seems like it would work great.

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InvisibleOlgualion
Shaman-In-Training
 User Gallery

Registered: 11/13/01
Posts: 1,253
Loc: Currently Earth...
Trusted Cultivator
Re: Liquid Mycelium Culturing [Re: Erawk]
    #504833 - 12/30/01 01:38 PM (22 years, 6 months ago)

The only disadvantage I can think of is that a contaminant in a suspension of liquid mycelium, can easily go unnoticed. This could lead to many jars contaminating at once. But so long as you are sterile and you are sure of the purity of your liquid culture, liquid mycelium is the way to go.

Oq


--------------------
Study the past...
See the future...

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Offlinezhukov
enthusiast
Registered: 12/20/00
Posts: 202
Loc: Australia
Last seen: 22 years, 3 months
Re: Liquid Mycelium Culturing [Re: Erawk]
    #505055 - 12/30/01 04:33 PM (22 years, 6 months ago)

If you can do it in a sterile environment - i suggest a well-disinfected glovebox! - it is MUCH faster than sp0res......100% in 9-10 days with BRF...........!:tongue:

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InvisibleTCatz
Manic
Registered: 11/09/01
Posts: 215
Re: Liquid Mycelium Culturing [Re: Erawk]
    #505080 - 12/30/01 05:01 PM (22 years, 6 months ago)

faaasst colinization times!
gots ta be clean though


--------------------
Flying in a Blue Dream

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Offlinecookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 22 years, 3 months
Re: Liquid Mycelium Culturing [Re: TCatz]
    #546521 - 02/10/02 10:40 AM (22 years, 5 months ago)

FAST? What recipe were you using fo your liquid culture?
how much agar did you inoculate (or mycelium) did you inucoulate the liquid with?

I have 2 with spores, and 2 with agar slabs.. neither look any good, really.
they are kindda cloudy, but i think thats more from the malt extract than anything
anyone have a picture of what it actually LOOKS like?
thanks

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OfflineShdwstr
FSRCanada
Male

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 7 months
Re: Liquid Mycelium Culturing [Re: cookiewhore]
    #546566 - 02/10/02 11:32 AM (22 years, 5 months ago)

Your liquid culture should look a little like this...



The jar on the right is after after 5 days (Maylasian). The one on the left is what can happen if your culture gets a little sloppy.



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Offlinecookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 22 years, 3 months
Re: Liquid Mycelium Culturing [Re: Shdwstr]
    #546981 - 02/10/02 07:34 PM (22 years, 5 months ago)

Holy crap.  heh, thanks for hte pic.  mine looks NOTHING like that.  I'm having excellent success with agar, but liquid just isnt going at all.
i was using 4g malt extract w/ 96mL of water.
autoclaved it, dropped a piece of mycelium in,  and i shake it every day.. its been going at least 2 weeks.. haha
i dont have any hope in it, just left it for the hell of it :smile:
Thanks man.

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OfflineShdwstr
FSRCanada
Male

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 7 months
Re: Liquid Mycelium Culturing [Re: cookiewhore]
    #547096 - 02/10/02 09:37 PM (22 years, 5 months ago)

What temperature are the jars at? I keep mine in an incubation room between 85 and 90. You should see a good growth starting in about 3 or 4 days.

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Offlinecookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 22 years, 3 months
Re: Liquid Mycelium Culturing [Re: Shdwstr]
    #547622 - 02/11/02 08:00 AM (22 years, 5 months ago)

My incubation chamber is at 85 degrees.
No problems there. i can completely colonize a pitre dish from an agar slab of B+ in 2 days, so shes flyin pretty good.

Must be something in the mixture.
I mixed up the 4g malt extract / 96mL of water, then autoclaved.
also, the top has a poly fill filter floss filter on it.

I WONDER what is going on here.

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Offlinekevman
journeyman
Registered: 12/22/00
Posts: 53
Last seen: 21 years, 9 months
Re: Liquid Mycelium Culturing [Re: cookiewhore]
    #548659 - 02/12/02 07:06 AM (22 years, 5 months ago)

I just use a half pint canning jar with one hole in the lid and a glass marble to agitate the myc..Put
about 1/4 filtered water and pressure cook for 15min. After the first use i store in the fridge...It lasts
for at least 1 month without sterility being an issue..
I take a fully colonized pf style cake with a sterile spoon and a clean room scoop up about a tbsp of
fresh myc.. into sterile jar. This is by far the easiest and most fullproof way to use the liquid myc..tek
I rarely if ever experience contamination....!!!

Edited by kevman (02/12/02 07:15 AM)

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