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InvisibleMmkay
newbie
Registered: 05/12/02
Posts: 47
2nd attempt is a bust
    #786038 - 07/30/02 09:28 AM (21 years, 7 months ago)

Please if someone can help me out here.. I thought I did everthing right this time.

I had 12 1/2 pint jars bought pre-filled/sterilized BRF from micronmagic
I innoculated all 12 with 3 syringes from sporeworks (mazatapec, ecuador, PR) Syringes were cleaned with lighter and alcohol swab.
I covered the tops of each with a small square of foil.
Jars were then placed in an incubator.

The incubator was made from 2 large rubbermaid tubs. One filled halfway with water and 1 cup hydrogen peroxide, and a fish tank heater on the bottom set to 82F. The second tub was set inside, then the jars placed in the 2nd tub. Tub was covered with blanket.

This has been sitting in my room for over a month, and not one of the jars has finished. Once the bottom half looked complete, i flipped the jars. Now i see 4 of them have green on the mycelium. I'm really upset, I thought things were going well. Please someone throw me a line!

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OfflineJohnnyR
mi cixelsyd

Registered: 07/07/02
Posts: 2,187
Loc: a hot place
Last seen: 18 years, 19 days
Re: 2nd attempt is a bust [Re: Mmkay]
    #786052 - 07/30/02 09:32 AM (21 years, 7 months ago)

"I had 12 1/2 pint jars bought pre-filled/sterilized BRF from micronmagic"

thats your problem..

make your own substrate and sterilize it yourself


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OfflineKilla420
Alien Lover
Male User Gallery
Registered: 06/26/02
Posts: 1,057
Loc: Down South
Last seen: 1 year, 3 months
Re: 2nd attempt is a bust [Re: Mmkay]
    #786053 - 07/30/02 09:33 AM (21 years, 7 months ago)

Sounds like you did everything right to me. Do you have a thermometer in the incubator? What does it read? I dont know what could have went wrong but, a month seems like wayyy to long for half pint jars. Why dont you just make you own substrate instead of buying it. It probally would be cheaper, and now you already have 12 jars.

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InvisibleMmkay
newbie
Registered: 05/12/02
Posts: 47
Re: 2nd attempt is a bust [Re: Killa420]
    #786160 - 07/30/02 10:21 AM (21 years, 7 months ago)

Yeah, maybe the stuff wasn't sterile to begin with. I just hate to buy a pressure cooker... :/

I took 3 of the cakes that were about 80% done and threw them in my terrium. Can I expect to see any growth out of them?

Thanks!

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Offlinecantankerous

Registered: 04/05/02
Posts: 522
Loc: hidden in your terrarium
Last seen: 5 years, 5 months
Re: 2nd attempt is a bust [Re: Mmkay]
    #786164 - 07/30/02 10:23 AM (21 years, 7 months ago)

you dont need a pressure cooker, read the teks.

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InvisibleTM
The Mind, The Many, The Music.
Male User Gallery

Folding@home Statistics
Registered: 06/11/02
Posts: 8,282
Loc: Under The Table And Dream...
Re: 2nd attempt is a bust [Re: Mmkay]
    #786251 - 07/30/02 11:15 AM (21 years, 7 months ago)

In reply to:

bought pre-filled/sterilized BRF from micronmagic



That's why they contaminated. I had the exact same thing happen, some green, some grey. Don't buy jars from Mycotek!


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"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

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Invisibleutopianglory
Spunkmuffin
Registered: 07/20/02
Posts: 965
Re: 2nd attempt is a bust [Re: Mmkay]
    #786362 - 07/30/02 12:15 PM (21 years, 7 months ago)

I have put unfinished cakes into a humid terrarium and had success. The growth is a lot quicker than the final stages of taking over the jar in the terrarium. The only worry is of course contamination so avoid opening the terrarium atall.

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Anonymous

Re: 2nd attempt is a bust [Re: Mmkay]
    #786478 - 07/30/02 01:20 PM (21 years, 7 months ago)

yeah, mix your own damn substrate fool!

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Offlineshroomsrgood
Stranger
Registered: 05/18/02
Posts: 16
Last seen: 21 years, 7 months
Re: 2nd attempt is a bust [Re: Mmkay]
    #786948 - 07/30/02 05:58 PM (21 years, 7 months ago)

pressure cookers are for yuppies... take your moms 3 piece steamer. put just enough water in it to cover like half an inch of your bottom jars. then put as many jars as you can fit in it. ( i can get 6) then boil the water and after it starts boiling wait an hour, remove the jars and put your other 6 in. wait til the next morning and innoculate those fuckers, but use the oven tek. i haven't had any contam's yet. good luck.

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InvisibleDarkmoon
Thinker

Registered: 11/17/99
Posts: 218
Re: 2nd attempt is a bust [Re: Mmkay]
    #787058 - 07/30/02 06:56 PM (21 years, 7 months ago)

How much were the jars? a pressure cooker would be a good investment no matter what, since it has other uses as well (damn good roast ) if your not sure your going to stick with the hobby just try a cheap one from Xmart or a Goodwill store are always good to check

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