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Registered: 07/19/02
Posts: 1
Last seen: 21 years, 2 months
Direct innoculation of Rye
    #758552 - 07/19/02 07:16 AM (21 years, 2 months ago)

Quite some time ago I read a post about a person scraping spores directly into his/her jars of rye as a method of innoculation. They argued that the water found in the syringes is nothing more than a convenient/easily sterilized medium to introduce the spores onto the rye. With proper aseptic technique, and a clean print, they thought that the spores could be scraped directly onto the substrate. I was wondering if anyone has any experience using this type of innoculation technique? Does it work? What are some of the pitfalls to look out for?

The reason I am asking is because when I use rye, I usually end up with either too much water in with the rye or not enough. I thought that by directly scraping sproes into the jar after pressure cooking, I could avoid adding more water, along with the spores, to the already saturated substrate.

Any advise will be greatly appreciated.

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Slave to theShroom

Registered: 09/19/01
Posts: 447
Loc: Midwest, US
Last seen: 16 years, 6 months
Re: Direct innoculation of Rye [Re: sector7g]
    #758796 - 07/19/02 09:20 AM (21 years, 2 months ago)

What kind of Rye:Water ratio are you using? My cat finds that one cup rye and one cup water in a quart jar seems just just enough. Never had a problem with the extra water from innoc, either.

You could scrape spores directly from a print into a jar, but it will take about 12-24 hours longer to germinate because the spores are going to be dehydrated and will need some time to rehydrate.

The main arguments, I think, against using stright sprores are as follows:

1. It will take longer to germinate, allowing competitors a head start.
2. You will probably have to open the jar wider and for a greater duration of time during innoculaion.
3. You actually don't need very many spores to innoculate some rye - using more might speed up colonization a bit, but not all that appreciably. Your print would probably go further if it's hydrated in a syringe of sterile water and injected in 1cc increments.


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