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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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failed agar and need advice
    #7545998 -

i had made agar plates to clone and the percentage was..
2 out of 10 jars didn't contaminate, shitty eh?
only one clone and one spore agar made it, the rest got this ugly pink mold and green mold
do i need to improve my pressure cooking time? maybe I'm not leaving it long enough, 20 minutes.
when i think about it, agar and mashed potatoe flakes probably have alot of endospores in them,,


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Re: failed agar and need advice [Re: wutang]
    #7546020 -

When cloning, especially outdoor fruits, I usually see contamination on 100% of the plates. That's the purpose of using agar. You can easily transfer healthy mycelium away from the contaminants, therefore cleaning up your culture. It usually takes three or four transfers before you've left the nasty stuff behind for good.
RR


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Re: failed agar and need advice [Re: RogerRabbit]
    #7546089 -

I was about to suggest the same thing, thats the great advantage of agar, is that a contaminated dish can be cleaned up until u have something usable, this has been suggested to me before with spore prints,grow the print out on agar in a petri, if it contams,do transfers till u end up with a clean sample.

Just remember you will still have to do this in a sterile enviroment


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Re: failed agar and need advice [Re: veda_sticks]
    #7546093 -

so if i have an agar plate with mycelium on it and the other side, mold, just cut off the mycelium and do it again? let me know plz


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Re: failed agar and need advice [Re: wutang]
    #7546188 -

Ive havnt yet done agar yet, but common sense tells me that you would take a small wedge as far away from the contam and transfer to another dish. Repeat until u have a clean sample.


Also think it would be a good idea to have a conrol dish of just agar, to make sure your mothods are not contaming ur project.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Re: failed agar and need advice [Re: veda_sticks]
    #7546290 -

all good advice from veda, as thats precisely what you want to do.
take a sample from as far away as possible (if its even possible), quickly and without touching anything else, transfer to new plate.

and a control plate is always a good idea, its also a good way of telling your spore load in any room.

in addition, if you are separating fungus from mold on agar, start isolating the fungus from the mold at the first signs of mold germination, well before any colors start to appear, this will hedge the bets in your favor, as the mold will only be able to spread thru direct contact.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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