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Amazon Shop for: Agar, Malt Extract, Petri Dish

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OfflineCultyVader
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Registered: 03/13/03
Posts: 595
Last seen: 4 years, 2 months
Agar culture highly contaminated
    #1409344 - 03/25/03 05:55 PM (13 years, 8 months ago)

I just started a culture a few (3) days ago and its contaminated (ants got around the edges due to my shitty sealing job). I was wondering if i can keep it and wait for mycelium to grow more, then cut it out out and try to transfer away the contaminates. Or, just toss it out now and start over. It hasnt gone for very long, so that wouldnt be too big of a problem.


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InvisibleRoadkill
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Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
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Re: Agar culture highly contaminated [Re: CultyVader]
    #1409400 - 03/25/03 06:18 PM (13 years, 8 months ago)

If its contaminated....I'd start over.

Stick your cultures in a sealed container...keeps bugs out.

Tip :
You can use black electrical tape to seal the petri dish.

good luck!


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineCultyVader
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Posts: 595
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Re: Agar culture highly contaminated [Re: Roadkill]
    #1409410 - 03/25/03 06:22 PM (13 years, 8 months ago)

Hey roadkill if your willing i have a few more questions about agar cultures
1. Best temp to incubate
2. Have you ever used just dogfood and agar before?
3. Light. Is it always a bad idea in this stage or not important?
4. Thie bigger the piece i throw in there the faster my results or am i just kidding myself?


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InvisibleRoadkill
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Re: Agar culture highly contaminated [Re: CultyVader]
    #1409477 - 03/25/03 06:40 PM (13 years, 8 months ago)

I incubate at room temp...74 degrees F.
I have wonderful growth at this temp.

I have never tried dogfood agar...and probably won't.
I have a dog and the food...but it seems like making it is a pain in the ass.
I'll stick to MEA and PDYA. :smile:

Well if you give the plates light you will have small mushrooms growing on them.
I keep mine in the dark.

Bigger has nothing to do with making agressive mycelium.
If you are cloning...cut out a small piece of the stem and peel the outside portion off...then dunk it in a mix of hydrogen peroxide and water before putting the clone on the agar.

I'll take a picture of a South African clone that I have on Agar now and post it in a few minutes.

good luck!
 


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineCultyVader
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Re: Agar culture highly contaminated [Re: Roadkill]
    #1409509 - 03/25/03 06:49 PM (13 years, 8 months ago)

cool thanks


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OfflineCultyVader
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Re: Agar culture highly contaminated [Re: CultyVader]
    #1409514 - 03/25/03 06:51 PM (13 years, 8 months ago)

OH YEA do i shove that little piece into the agar or just drop it on the surface?


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InvisibleRoadkill
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Re: Agar culture highly contaminated [Re: CultyVader]
    #1409550 - 03/25/03 07:01 PM (13 years, 8 months ago)

just lay the peice gently onto the agar.

South African Clone on MEA.


South African on PDYA...1st transfer...this strain is amazing!


As you can see from the pictures the South African is very agressive!!


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineCultyVader
Dark SporeApprentice

Registered: 03/13/03
Posts: 595
Last seen: 4 years, 2 months
Re: Agar culture highly contaminated [Re: Roadkill]
    #1409564 - 03/25/03 07:05 PM (13 years, 8 months ago)

Wow That is truly amazing!! How do you deal with water condensation it looks to me like youve got none at all.

P.S. Im very jealous of those nice looking petri dishes Pyrex i assume?


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InvisibleRoadkill
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Re: Agar culture highly contaminated [Re: CultyVader]
    #1409640 - 03/25/03 07:33 PM (13 years, 8 months ago)

I don't get any condensation on my plates. :smile:

I have Pyrex and Kimtex petri dishes...and some poly autoclaveable ones that I haven't tried yet.
The poly are expensive...they were given to me by a good friend. :smile:

I'm pretty impressed with the South African!

good luck to you!


 


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisiblemobyd
mad agar worker
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Re: Agar culture highly contaminated [Re: Roadkill]
    #1409674 - 03/25/03 07:46 PM (13 years, 8 months ago)

I?ve tried the dogfood agar. But don?t try this at home, it smells so disgusting....

But the mycelia likes it :grin:


--------------------
Sorry, my english is really bad!

If you like my work, rate me....


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OfflineCultyVader
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Re: Agar culture highly contaminated [Re: mobyd]
    #1409686 - 03/25/03 07:51 PM (13 years, 8 months ago)

yah i noticed that right away it was downright nasty. SO made two new plates both with chunks from the inside of the stems and put them in the petris using the oven tek. The plates were sealed using Electrical tape (Thanks ROadkill)


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Amazon Shop for: Agar, Malt Extract, Petri Dish

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