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OfflineMacey Howard
Formally MOE HOWARD
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Registered: 07/03/99
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Post deleted by Moe Howard
    #680751 - 06/15/02 06:21 PM (15 years, 17 days ago)



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InvisibleChampion des Champignons
long standing member;)

Registered: 07/27/00
Posts: 2,679
Loc: Alba
Re: Letting spores drop directly on agar. [Re: Macey Howard]
    #680765 - 06/15/02 06:31 PM (15 years, 17 days ago)

I think that would produce waay too many substrains on one plate, kind of defeats the purpose of agar culture?


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OfflineDinoMyc
Ipsa scientiapotestas est
Registered: 11/14/99
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Re: Letting spores drop directly on agar. [Re: Macey Howard]
    #680782 - 06/15/02 06:41 PM (15 years, 17 days ago)

I agree with you. A waste.. also, with so many different sets of genes, there could be some delay as the dominant ones emerge.


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OfflineBanshee
wannabepsilocybe

Registered: 06/23/01
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Re: Letting spores drop directly on agar. [Re: Macey Howard]
    #680789 - 06/15/02 06:44 PM (15 years, 17 days ago)

a cap isn't so bad for experimenting. I think FRESHER SPORES=FASTER COLONIZATION... but again there are other factors, you might let the spores need some time to settle and let them get hungry... (?).

It's good to experiment, and learning new things everyday isn't a waste. What if it works better than you guys expected to? Keep posting.

But the spores should all be thrown in @ once right?


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OfflineMacey Howard
Formally MOE HOWARD
Female

Registered: 07/03/99
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Loc: Georgia
Last seen: 9 months, 25 days
Post deleted by Moe Howard [Re: Banshee]
    #684237 - 06/17/02 05:02 PM (15 years, 15 days ago)



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OfflineAnnoA
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Re: Letting spores drop directly on agar. [Re: Macey Howard]
    #684784 - 06/17/02 08:59 PM (15 years, 15 days ago)

You?re bot too far away with your thoughts.
It is actually done in the indutry, where they suspend a cap on a few wires upon a beaker containaing nutrient soulution to make a multispore inoculation(basically they make a nutrient enhanced spore suspension that germinates, then they inoculate bulk substrates)
That?s at least what Stamets tells in his books.


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InvisibleLanaV
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Registered: 10/28/99
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Re: Letting spores drop directly on agar. [Re: Anno]
    #686581 - 06/18/02 06:34 PM (15 years, 14 days ago)

Yet again, Anno is one sharp cookie (I've never eaten a sharp cookie and hope not too )

There are ...hmm? I can't rememeber what their actual name is but its just a ring that has wire strung over top of it. They are mostly stainless steel or titainium. I've seen them before and for some reason they are VERY expensive.

They're meant for culture work. Plant tissue especially.

Lana


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OfflineDinoMyc
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Re: Letting spores drop directly on agar. [Re: Anno]
    #686719 - 06/18/02 07:50 PM (15 years, 14 days ago)

To make essentially a mycelial solution.. not quite the same as a single dish with so much confusing matter on hand..
imagine a room (if your male... or female and interested) with a thousand women.. who do you chose for first? second??.. and vice versa... i think its the same deal here.. with so many different 'strains' vyeing for the dominance, it could be a problem.. then again, perhaps the dominant strain will emerge quicker than with less competition, and the dish will prove most fruitful.
Yes the equipment is expensive. So is everything with such a niche market.. have you looked up high frequency adapters lately? $$$$$ ! everything for an experimenter will be expensive, because you and a selected few other people have to pay for several engineers and perhaps a few buisnessmen(or women).. c'est la vie.


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OfflineAnnoA
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Re: Letting spores drop directly on agar. [Re: DinoMyc]
    #687029 - 06/18/02 10:40 PM (15 years, 14 days ago)

What Stamets writes:

"Theoretically, the germination of spores in mass creates multitudes of strains that will compete with one another for nutrients. This has been long accepted as one of the Ten Commandments of Mushroom Culture. Scientists in China, whose knowledge had not been contaminated by such preconceptions first developed spore-mass inoculation techniques to an industrial level. Only recently have Western mycologist recognized that a large community of spore matings behaves quite differently than paired individuals. San Antonio and Hanners (1984) are some of the first Western mycologists to realize that grain spawn of Oyster mushrooms could be effectively created via spore-mass inoculation.
The most aggressive strains out-race the least aggressive strains to capture the intended habitat.
Recent studies have shown that these aggressive strains overpower and invade the cellular network of competing strains.
Dr. Alan Rayner (1988) in studies at the University of Bath described this form of genetic theft as "non-self fusions" between genetically different mycelial systems within the same species. This ability to adapt has made fungi one of the most successful examples of evolution in the biological arena.
Spore-mass fermentation techniques are not yet widely used by North American or European cultivators. Concern for preserving strain stability, lack of experience, equipment, and intellectual conflicts are contributing factors. In mushroom culture, intransigence to new ideas has prevailed, often because the slightest variation from the norm has resulted in expensive failures. Since the health of any economy is based on its diversity, the emergence of organically minded gourmet mushroom growers is creating a fertile intellectual habitat for many innovative technologies."


Edited by Anno (06/18/02 10:43 PM)


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