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Offlinehomebrew
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Registered: 06/02/01
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Last seen: 13 years, 6 months
Agar help
    #513021 - 01/07/02 10:55 AM (14 years, 10 months ago)

I made about a dozen plates over the weekend using a tryptone/yeast-extract medium. All plates and medium were autoclaved @ 250 F for 30 min. With a sterile innoculation loop, I swiped directly from the spore print to the agar. Innoculated plates are being stored in darkness around 80 F. I checked them this AM and am not seeing any indication of growth. I know that I am probably being prematurely worried, but I have a couple of questions after the fact. Should I have hydrated the spores prior to the agar transfer? How long should I wait before my attempt is ruled a failure? I poured my plates a little thick (~ 3 mm), will the mycellium penetrate the agar, or just grow across the surface.

Thanks everyone.


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Invisiblear393
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Registered: 01/27/00
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Re: Agar help [Re: homebrew]
    #513325 - 01/07/02 04:34 PM (14 years, 10 months ago)

depending on how old the spres are it could take 5-7 days. they will rehydrate on the agar. and it grows across the agar. 250 for 30 is a little long, 15 minutes should be it.

I would wait at least ten days before you give up on the plates. but its not a bad thing to pour your plates a little think (my cat's plates are usually around 4 mm) just because the mycelium will have a ready source of water when you drop your agar into your grain. If your agar is too thin it will dry out before the mycelium really gets a good grip on the grain and can sustain itself.

peace, ar393


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Offlinehomebrew
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Registered: 06/02/01
Posts: 117
Last seen: 13 years, 6 months
Re: Agar help [Re: ar393]
    #513914 - 01/08/02 02:11 AM (14 years, 10 months ago)

Thanks for the feedback, I will keep an eye on them over the next week.

The big question was medium depth: I ran out of big (10 cm) petri dishes and am using small (5 cm) dishes. I was hoping that I could make up in lost surface area by depth.

Thanks


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