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North Spore Shop: Injection Grain Bag

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Offlinephreedom
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Post deleted by MOE THE MAD SCIENTIST
    #1107772 - 12/03/02 06:41 PM (18 years, 7 months ago)



Edited by phreedom (12/03/02 07:19 PM)


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InvisibleJoshua
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Re: agar didn't solidify [Re: phreedom]
    #1108125 - 12/03/02 09:54 PM (18 years, 7 months ago)

You didn't have a high enough agar/water ratio. How many grams of each agar did you add to what volume of water?

Joshua


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Edited by Joshua (12/03/02 10:19 PM)


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Offlinephreedom
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Post deleted by MOE THE MAD SCIENTIST [Re: Joshua]
    #1108934 - 12/04/02 03:07 AM (18 years, 7 months ago)



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OfflineAnnoA
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Re: agar didn't solidify [Re: phreedom]
    #1109044 - 12/04/02 04:03 AM (18 years, 7 months ago)

> but i couldn't pc until a few days later.

You mean you mixed the preprepared agar and water, and then left it sitting for a few days?

That?s bad, bad....
Start anew.


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OfflinestonErollEr1
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Re: agar didn't solidify [Re: Anno]
    #1109481 - 12/04/02 10:53 AM (18 years, 7 months ago)

It is? I didnt know that :blush:..nice to know that before it actually happends..usually i do PC my agar right away though.[so far.]
Anno: Do you know why?[does AGAR loose it solidyfing properties when left sitting in water?hmm..?]

peace...


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OfflineRaadt
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Re: agar didn't solidify [Re: stonErollEr1]
    #1109546 - 12/04/02 11:41 AM (18 years, 7 months ago)

oxidization? breakdown?

my guesses.


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OfflineAnnoA
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Re: agar didn't solidify [Re: stonErollEr1]
    #1109644 - 12/04/02 12:44 PM (18 years, 7 months ago)

What you did was let a nourishing solution(agar agar, sugar(glucose, maltose) and yeast extract and water sit at room temperature.
I?m sure you got all sorts of nasty contaminats growing in the solution if you let it stand at room temperature for a few days....

You can let agar sit for a long time, but only if it?s sterilized before.


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OfflinestonErollEr1
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Re: agar didn't solidify [Re: Anno]
    #1112827 - 12/05/02 08:49 AM (18 years, 7 months ago)

Ok, i see that having an unsterilized highly nutrious mix sitting in room temp. for a couple of days cause contams..but do not sterilizing it cause that it?s not solidyifing when it is PC?d later on?
IE are the solydifing properties lost?
peace...


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OfflineAnnoA
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Re: agar didn't solidify [Re: stonErollEr1]
    #1112848 - 12/05/02 09:12 AM (18 years, 7 months ago)

agar has likely been digested by some bacteria, my guess.


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OfflineEvilGir
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Re: agar didn't solidify [Re: Anno]
    #1113560 - 12/05/02 01:37 PM (18 years, 7 months ago)

Could of been contaminated by a bacteria and it byproduct could of increased the acidity of the agar which would stop it from solidifying.


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North Spore Shop: Injection Grain Bag


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