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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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An odd colonization phenomena with old LC
    #6365648 -

Exhibit A:



Two jars of WBS.  Both jars prepped on the same day, using the same batch of hydrated WBS, PC'd at the same time even.  All things equal so far.  On the left, inoculated with multispore.  On the right, inoculated with a 6-month old LC (originally from a clone) that has been under refrigeration for nearly as long.  Both inoculated the same day, and "incubated" at room temperature.  The difference between the two is even more drastic in person, its just hard to capture that with a camera flash going off against a glass surface :wink:

Here's another shot without the flash, should be even more obvious:


I tried letting the jar on the right sit around for an entire week after it "appeared" to be fully colonized to see if it would whiten up some, but no go.  I started seeing the telltale signs that that baby was as colonized as she was going to get - rhizomorphs forming, and a little bit of metabolite accumulation down at the bottom of the jar.

Notice the multispore jar looks nice and white and normal, while the LC jar seems to have whispier growth, with some grains still showing through.  I have noticed a trend that the older an LC gets, the weaker the growth appears.  The first month or two, the culture produced dense, white, healthy growth.  Then it gradually declined... and declined some more... until now as you see its pretty drastic, you can  clearly make out the kernels all too well through the colonization.

Exhibit B:


Some of the sketchy LC-inoculated spawn running through a coir substrate.  Again, light and whispy growth rather than the thick dense snow-white appearance I normally enjoy.  Again, rhizomorphs forming, telling me we're nearing full colonization.  Yet there's still a lot of brown color showing through from the coir, and you can *still* make out a lot of the grains.

Now here's the really confusing part.  Smells perfetly mushroomy.  A grow just prior to this showed the same light colonization symptoms - and fruited beautifully, no different than the first time the LC was used many months ago.  So despite it looking unhealthy and appearing very different, it still performs equally as well.  I'm puzzled.

It would seem to me a culture after a long period of storage is either viable, or it is not.  I can't come up with any reason why its growth characteristics would change over time, especially considering the fact that its source was a clone and the genetics should be consistent... and my methods have remained pretty consistent.  Apparently its not so black and white.

Anybody experience similar?  Any thoughts as to how this could possibly be?  I'm a little puzzled to say the least, but considering it still turns out fine and fruits normal, I'm not so sure I should even be concerned  :confused:  Results are results, but its just getting to a point where the spawn looks so sketchy that I'm tempted to not bother with it...


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Cloning the easy way with LC - newbie friendly pictorial tek

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Re: An odd colonization phenomena with old LC [Re: creamcorn]
    #6365676 -

Your old LC is fighting bacteria, as can be seen in both of those jar pictures, even the one with flash. That's also the reason you're seeing a buildup of metabolites in the bottom of the jar. That may or may not prevent fruiting, but if you tried to use that old jar for grain to grain transfer, you'd see what I mean. The bacteria would completely take over.


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Re: An odd colonization phenomena with old LC [Re: RogerRabbit]
    #6365705 -

I had an inkling it might be something like that, though I've never known a bacteria to not completely take over the place when it came to sterile grains.  Some of the grains do look a bit wet-spotty, like the one mushed up against the jar just to the left of the glass ridge, little more than half way down...  incidentally my incubator broke down yet again which is why these were done at room temp, and room temp has been about 68F, so it makes some sense that maybe the bacteria didn't spiral out of control so quickly because of the lower temperature.

I know I've dipped in to the particular LC well over a dozen times now so its certainly reasonable something nasty has gotten in there by this point.

Interesting that it still colonizes bulk and still fruits fine.

Also interesting that the small yellow grains seem to be effected the most... I guess this bacteria is a picky eater. :smile:


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Cloning the easy way with LC - newbie friendly pictorial tek

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