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buggers
Mushroom CloudLayin'Motherfucker


Registered: 03/20/05
Posts: 324
Last seen: 10 years, 7 months
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mixing cloned myc with multispore myc
#5376153 - 03/07/06 10:49 PM (17 years, 10 months ago) |
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quick hypothetical question. i just spawned 14 quarts of WBS colonized by Yangoon Burmese mycelium that was initially cultured on agar from a clone of a fresh fruitbody to 4 dishpan type tubs of pasteurized horse poop (sub depth around 5 inches each). I've got 2 quarts of multispore inoculated WBS of the same strain just kicking around, and i was wondering if cloned myc is compatable with multispore...
say i'd spawned a tub of poo with 2 quarts cloned myc and 2 quarts multispore myc (same strain of course)... what would happen? would they compete for nutrients, or would they fruit as normally as any multispore inoculated myc is capable of doing?
i would have spawned a fifth tub using some of each type of myc but was unsure what the ramifications would be of using cloned myc in conjunction with multispore myc... so i opted to use .5 quart more of the cloned spawn for each tub in hopes of lessening the substrate colonization time (which was probably smart considering the depth of the sub)...
the multispore will probably sit around til it either fruits invitro, i get around to pasteurizing more poo, or i use it for grain to grain transfers (if i took this route it would be second generation G2G spawn because these were made with G2G in the first place... this is ok right? after 3rd time you get problems right?).
sorry if this post is disorganized... too many PBR's.
thanks guys.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: mixing cloned myc with multispore myc [Re: buggers]
#5376850 - 03/08/06 08:39 AM (17 years, 10 months ago) |
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Multispore inoculation gives you dozens, or even hundreds of strains. The cloned tissue is simply one more strain. It will assimilate into the mix without problems. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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buggers
Mushroom CloudLayin'Motherfucker


Registered: 03/20/05
Posts: 324
Last seen: 10 years, 7 months
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Re: mixing cloned myc with multispore myc [Re: RogerRabbit]
#5379677 - 03/08/06 11:00 PM (17 years, 10 months ago) |
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thank you rodger... thats what i thought but wasnt exactly sure... you seem to have the answer to a lot of my questions i've had lately... would you care if i start PMing you? lol it would be a lot easier than looking for a post that i made days ago that i'd assumed never got responded to.
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