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Amazon Shop: ½ Pint Jars, Agar, Rye Grain, Wild Bird Seed

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Offlinemavix
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Registered: 01/28/04
Posts: 91
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agar isolations vs multispore = no contest for comparison?
    #2346919 - 02/17/04 07:20 PM (13 years, 7 months ago)

I was driving home yesterday night talking with a friend, and this idea came to mind, lemme know if i'm way off or not.

Basically, anyone who is doing 'experiments' on multispore innoculation to compare growth changes etc. isnt really going to get an accurate result due to the fact that it is a multi-spore innoculation. and thus the control is lost.

So my thought for conducting tests to compare different mediums was this. (something i'd like to do once i'm set up for agar work)
1, Isolate via agar substrain that meets requirements
2, clone substrate to jars of (whatever) for a base-line, incubate using set perameters of constant temp etc. then case and fruit. recording data as base-line
3, using same clone, attempt various techniques such as the coffee, hominy, wbs vs millet vs Rye grass seed vs rye grain

in my line of thought.
I was thinking that this is the only way you are going to get an accurate comparison of how each medium works (with repitition for proof of results)

not being scientificly minded can someone point me in the right direction if i'm 3 pages shy of a full novel.

thanks


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-- mavix


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InvisibleMagashM
Da Bud Guru
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Registered: 07/25/02
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Loc: Near Hilo
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Re: agar isolations vs multispore = no contest for comparison? [Re: mavix]
    #2347494 - 02/17/04 10:12 PM (13 years, 7 months ago)

"in my line of thought.
I was thinking that this is the only way you are going to get an accurate comparison of how each medium works (with repitition for proof of results)"

I fully agree with you on this.


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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


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Invisiblesimplemachine
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Registered: 09/14/03
Posts: 1,981
Re: agar isolations vs multispore = no contest for comparison? [Re: mavix]
    #2347640 - 02/17/04 11:10 PM (13 years, 7 months ago)

Would senesence be a problem when going through that many clonings?


SM


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Amazon Shop: ½ Pint Jars, Agar, Rye Grain, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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