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Psychoslut
The Mother Fucking Bear-o-dactyl

Registered: 12/10/02
Posts: 20,968
Loc: all up in ya

Re: ADHESIVE FILTER PATCH (cheap)

[Re: agar]
#5135260 - 01/03/06 02:41 PM (20 years, 7 months ago)

agar said:

3M Micropore tape.
It is all over ebay.
The 1 inch wide stuff comes in boxes of 12 rolls.
(30 ft per roll)

Quart wbs jars, all with lid holes covered with it & coffee filter dust covers.
PC time was 90+ minutes at about 18 psi.
Lids were 1/2 turn loose & tightened when taken out of PC, then shaken.

EDIT TO ADD:

This photo clearly shows jars are sideways, Backup.

--------------------

[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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backupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else

Re: ADHESIVE FILTER PATCH (cheap)

[Re: Psychoslut]
#5135268 - 01/03/06 02:43 PM (20 years, 7 months ago)

ohhh, they are laying on top of other jars aren't they. hmmm...I wouldn't see a prob with it and apparently neither does agar.

:peace:

:peace:

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FooMan

Registered: 02/02/05
Posts: 8,957
Loc: Earth

Re: ADHESIVE FILTER PATCH (cheap)

[Re: agar]
#5135269 - 01/03/06 02:43 PM (20 years, 7 months ago)

Can I swap out my weight for a "toggle pressure release valve"? Or is this something the PC needs to come with. I've got an AA925 with a weight. That pressure release valve looks like it would come in handy. :thumbup:

:thumbup:

--------------------

Quick WBS Prep

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FooMan

Registered: 02/02/05
Posts: 8,957
Loc: Earth

Re: ADHESIVE FILTER PATCH (cheap)

[Re: Psychoslut]
#5135280 - 01/03/06 02:45 PM (20 years, 7 months ago)

Uhhhhhhhh. I don't think it would be a big deal either way. Upright, sideways, 45 degrees, backupwards :smirk:

:smirk:

--------------------

Quick WBS Prep

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Wronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 15 years, 2 months

Re: ADHESIVE FILTER PATCH (cheap)

[Re: backupwards]
#5135281 - 01/03/06 02:46 PM (20 years, 7 months ago)

This may be a case for the supreme court. :lol:

:lol:

Just kidding you guys.

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Psychoslut
The Mother Fucking Bear-o-dactyl

Registered: 12/10/02
Posts: 20,968
Loc: all up in ya

Re: ADHESIVE FILTER PATCH (cheap)

[Re: Wronguy]
#5135289 - 01/03/06 02:50 PM (20 years, 7 months ago)

Apparently Stamets believe that vertical, horizontal and 45 degree angles all make different qualities of spawn, otherwise why would he suggest the 45 over vertical?

When I used to accidentally get too much moisture in the jar, and the very bottom had a hard time colonizing after the rest of the grain was 100% id put it sideways and that helped the bottom colonize.

--------------------

[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

Extras:

FooMan

Registered: 02/02/05
Posts: 8,957
Loc: Earth

Re: ADHESIVE FILTER PATCH (cheap)

[Re: Psychoslut]
#5135300 - 01/03/06 02:53 PM (20 years, 7 months ago)

Lay those jars down however you want Psychoslut. We'll still love you. :grin:

:grin:

I've always kept them standing upright and had no problems, but I'm no Stamets.

--------------------

Quick WBS Prep

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Jaeger
Dreamer

Registered: 10/01/05
Posts: 960

Re: ADHESIVE FILTER PATCH (cheap) *DELETED*

[Re: FooMan]
#5135366 - 01/03/06 03:20 PM (20 years, 7 months ago)

Post deleted by Jaeger

Reason for deletion: .

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Liquidkick
H2O

Registered: 05/03/02
Posts: 2,635

Re: ADHESIVE FILTER PATCH (cheap)

[Re: Jaeger]
#5135839 - 01/03/06 05:35 PM (20 years, 7 months ago)

Can i use this stuff for LC jars? Like putting the tape over my holes? It doesn't contam if i get it wet with the LC?

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capnstemz
Stranger

Registered: 11/30/05
Posts: 78
Last seen: 3 years, 4 months

Re: ADHESIVE FILTER PATCH (cheap)

[Re: agar]
#5138751 - 01/04/06 01:12 PM (20 years, 7 months ago)

is this the stuff?

http://www.cshyde.com/polyeste.htm

silicone adhesive, made out of spun polyester.

I know, not white but does meet the specs you have so far given.

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Wronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 15 years, 2 months

Re: ADHESIVE FILTER PATCH (cheap)

[Re: capnstemz]
#5138804 - 01/04/06 01:25 PM (20 years, 7 months ago)

If it's used in Powder Coating, Spray Coating, and Plating, as the website indicates, it may not be very porous. The biggest question I think is will that tape allow for gas exchange?

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agar
old hand

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else

Re: ADHESIVE FILTER PATCH (cheap)

[Re: Psychoslut]
#5139151 - 01/04/06 02:41 PM (20 years, 7 months ago)

Reason top layer of jars is horizontal is............. :rolleyes:

:rolleyes:

That is a standard locking type metal 4 drawer 3 foot deep filing cabinet.

Can fit about 28 jars per drawer.

Once jars are 98 to 100% colonized, I remove them from the incubator & store them in filing cabinets, in the garage, where winter temps are mid 40 - 50F.

That stalls them until I get around to using them.
I store colonized spawn bags - the same way.

--------------------

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capnstemz
Stranger

Registered: 11/30/05
Posts: 78
Last seen: 3 years, 4 months

Re: ADHESIVE FILTER PATCH (cheap)

[Re: agar]
#5139569 - 01/04/06 04:38 PM (20 years, 7 months ago)

what about the filters?

Extras:

hyphae
born to grow

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 15 years, 3 months

Re: ADHESIVE FILTER PATCH (cheap)

[Re: armedia]
#5139882 - 01/04/06 05:54 PM (20 years, 7 months ago)

armedia said:

Does anyone use this to wrap Petri plates? I wonder how it compares to Parafilm. Is it perhaps too porous for agar cultures?

- A

Here is where Glad ClingWrap works awesome as parafilm it is gas permeable unlike most all other clingwraps. Just cut a roll into 1" sections.

--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:

:wink:

Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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backupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else

Re: ADHESIVE FILTER PATCH (cheap)

[Re: hyphae]
#5140347 - 01/04/06 07:25 PM (20 years, 7 months ago)

nice tip hyphae, thanks.

:peace:

:peace:

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capnstemz
Stranger

Registered: 11/30/05
Posts: 78
Last seen: 3 years, 4 months

Re: ADHESIVE FILTER PATCH (cheap)

[Re: backupwards]
#5143765 - 01/05/06 04:03 PM (20 years, 7 months ago)

ah ha!
found 'em

Sorry agar.

http://www.tissuequickplantlabs.com/amdpg5c.htm

Looked for night and day for these things.

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Jaeger
Dreamer

Registered: 10/01/05
Posts: 960

Re: ADHESIVE FILTER PATCH (cheap)

[Re: capnstemz]
#5143787 - 01/05/06 04:13 PM (20 years, 7 months ago)

That is pretty expensive though!!

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Wronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 15 years, 2 months

Re: ADHESIVE FILTER PATCH (cheap)

[Re: Jaeger]
#5143873 - 01/05/06 04:48 PM (20 years, 7 months ago)

Nice find, but Jaeger is right, $1.14 a patch hardly seems worth it. There has got to be someone else out there who has a link to something similar made in the US.

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Premedman1
Assistant to the insistent

Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity

Re: ADHESIVE FILTER PATCH (cheap)

[Re: Wronguy]
#5144355 - 01/05/06 07:08 PM (20 years, 7 months ago)

US company in Kent, WA. A wide selection of diameters and micron pore sizes. Polyester, polypropylene, polycarbonate, etc.
HERE :thumbup:

:thumbup:

--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.

Extras:

Wronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 15 years, 2 months

Re: ADHESIVE FILTER PATCH (cheap)

[Re: Premedman1]
#5144377 - 01/05/06 07:14 PM (20 years, 7 months ago)

Great link, seriously. Now all I have to do is get a degree in biology and my masters in engineering so I know the correct micron pore size to order. :wink:

:wink:

What a pain in the ass.

Extras:

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Shop:

Isolated Cubensis Liquid Culture For Sale

Unfolding Nature: Being in the Implicate Order

Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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