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OfflinePhishinPhriendly
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Registered: 11/14/01
Posts: 27
Last seen: 17 years, 6 days
Tap water bad?? Advice Please!
    #478169 - 12/03/01 12:11 PM (17 years, 15 days ago)

About a month ago, I decided to attempt to grow some mushrooms. I got all my supplies and evrything. After some intense searching, I decided the best strain for me would be the Thia Koh Samui. I got my syringe from the lilshopofspores (great service btw).

I made 7 brf/verm jars, boiled them for about 90 min and let them cool. Innoculated 6 jars 7hrs later and waited. After about 4-5 days I saw some growth, but it was blue!? In EVERY single jar where I innoculated it was blue. Now its been about 8-9 days and the blue has grown and taken over about 60% of the jar. I dont think its a contam b/c I didn't innoculate one jar and it looks fine. No growth in it at all!! And all the Jars smell great. Seems odd to me that all 6 jars I innoculated had the EXACT same growth.

No disrepect to Lilshopofspores, but could the Syringe be contaminated, or is it that I used tap water?

I saved half of the syringe in case something like this happened and I'm gonna buy some distilled water and prepare 2 more jars.

Any ideas..thanks.


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OfflineAnnoA
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Registered: 06/18/99
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Re: Tap water bad?? Advice Please! [Re: PhishinPhriendly]
    #478240 - 12/03/01 01:01 PM (17 years, 15 days ago)

Well, if the control jar didn?t show any contamination, the contaminants needed to enter the jars at the moment of inoculation. So either the air where you inoculated the jars beared a heavy contaminats load, the syringe needle was dirty on the outside or the syringe contents were bad.


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OfflinePhishinPhriendly
newbie
Registered: 11/14/01
Posts: 27
Last seen: 17 years, 6 days
Re: Tap water bad?? Advice Please! [Re: Anno]
    #478253 - 12/03/01 01:08 PM (17 years, 15 days ago)

Thanks Anno
I heated up the needle between each jar (red hot).
I read that some people wipe the needle w/ Iso. Alcohol (99%)
Is this a better method? Shouldn't the 1/2 inch layer of plain Verm protect me from Contams? And I innoculated them in the living room. Thought it was clean..perhaps not! I guess I'll use the "inside the oven innoculation" method.


--------------------
His eyes were clear and pure, but his mind was so deranged.


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OfflineAnnoA
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Re: Tap water bad?? Advice Please! [Re: PhishinPhriendly]
    #478256 - 12/03/01 01:12 PM (17 years, 15 days ago)

>I heated up the needle between each jar (red hot).
That?s ok.

>I read that some people wipe the needle w/ Iso. Alcohol (99%)
>Is this a better method?
No, heating is just fine.

>Shouldn't the 1/2 inch layer of plain Verm protect me from Contams?
To some extent, yes.


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OfflineSCAD_Shroomer
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Registered: 07/22/01
Posts: 41
Loc: Savannah, Georgia, USA
Last seen: 17 years, 14 days
Re: Tap water bad?? Advice Please! [Re: PhishinPhriendly]
    #478270 - 12/03/01 01:44 PM (17 years, 15 days ago)

I used straight tap water to fill my syringes once and all the jars got cob web mold. I used the same tap water to make new syringes, only this time I boiled it first, and no problems.


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OfflinePhishinPhriendly
newbie
Registered: 11/14/01
Posts: 27
Last seen: 17 years, 6 days
Re: Tap water bad?? Advice Please! [Re: SCAD_Shroomer]
    #478291 - 12/03/01 02:24 PM (17 years, 15 days ago)

Thanks SCAD
I did some searches in the Forum and seemed to have mix reviews on Tap water. I think I'll do the next 2 jars w/ Spring water and innoculate them in the oven. If my syringe is contaminated, do you think LSOS will help me?

Also, after innoculating, leave the tape on or off!? I currently have the Jars in my Terrium w/ a heating pad to keep the temp up around 80-85 degrees. Any suggestions to help colinization on this next batch?
Thanks
JT


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His eyes were clear and pure, but his mind was so deranged.


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OfflinegeokillsA
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Registered: 05/08/01
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Re: Tap water bad?? Advice Please! [Re: PhishinPhriendly]
    #478336 - 12/03/01 03:17 PM (17 years, 15 days ago)

>If my syringe is contaminated, do you think LSOS will help me?

most likely



>leave the tape on or off!?

people seem to have better luck with the tape off...



as for tap water, plenty of people use tap water without issue.






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