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Offlinekspoker
Stranger
Registered: 12/15/02
Posts: 2
Last seen: 14 years, 7 months
Possible contamination questions... first timer!
    #1143344 - 12/15/02 02:59 PM (14 years, 8 months ago)

Hello,

Recently I ordered a spore syringe of Mazatapec to pursue mushroom growing. I got the syringe finally and completed everything a couple nights ago. Following are some guidelines I used to make the substrate jars and innoculation, as well as incubation.

-Washed all jars, utensils, everything with soap, then sanitized with alcohol, and kept everything on a sanitary cloth in the warm oven to prevent germs from falling on anything, kept it at a low temperature, just so warm air was rising.

-Mixed everything seperate, substrate jars that is. Sterilizing everything after each go. I sterilized the vermiculite and brown rice in the oven for 30 minutes before mixing, and mixed everything on the oven lid to prevent airborn contaminents from entering. I liberally sprayed lysol in the air every 5 minutes as well, as well as sanitizing my hands after every thing i touched.

-Put the jars in a sterilized rubbermaid inncubator, ala Alien.

-1cc in each jar, inccubator at 80 degrees.

-steamed the jars in a good pan w/ lid for 1 hour boiling water. made sure no water got in jars.

MY WORRIES:

-I used tap water for the substrate jars, did not boil water... IS THIS BAD?

Thanks!


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Offlinejoeshitragpicker
Home Sweet Home

Registered: 10/17/02
Posts: 1,265
Loc: Atlanta
Last seen: 3 years, 10 months
Re: Possible contamination questions... first timer! [Re: kspoker]
    #1143350 - 12/15/02 03:01 PM (14 years, 8 months ago)

you will be fine.


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Offlinesocratesmind
old hand

Registered: 02/22/02
Posts: 1,193
Loc: in your house :)
Last seen: 11 years, 1 month
Re: Possible contamination questions... first timer! [Re: kspoker]
    #1143361 - 12/15/02 03:02 PM (14 years, 8 months ago)

everything sounds fine and is probably an overkill. as long as you didn't pc/steam cook the jars after u innoculated? don't use the oven as means of a sterilization method. checkout www.fanaticus.com and follow their pf-tek. great for newbs.


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Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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OfflineAngry Mycologist
Spontaneouslycombusting

Registered: 11/24/02
Posts: 1,282
Loc: Galapagos
Last seen: 14 years, 3 months
Re: Possible contamination questions... first timer! [Re: kspoker]
    #1143363 - 12/15/02 03:03 PM (14 years, 8 months ago)

Yea, you should be fine.


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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Offlinekspoker
Stranger
Registered: 12/15/02
Posts: 2
Last seen: 14 years, 7 months
Re: Possible contamination questions... first time [Re: socratesmind]
    #1144126 - 12/15/02 08:00 PM (14 years, 8 months ago)

Oh ya,
I found brown rice with no preservatives in it and I used my chopper to chop it up... It got the rice into a nice powder, but there are still whole pieces of rice clearly visible in the jars, I don't think this matters, does it?

Also,
I steamed the jars with loose lides (before innoculation!) with tape covering the holes.. When I innoculated, I did so on the oven door, with heat rising out. I sanitized the top of each lid with a lysol wipe before sticking the syringe in each jar. I then innoculated and recovered the jars with the tape and put them in the inncubator.. My thoughts are this:

I am planning on removing the tape when the jars are about 1/2 way colonized. There are different teks on shroomery.com, and each one has differing opinions on this. Some say to leave the tape on the whole time, while the mmgg (the one I used), says to keep the holes untaped right after innoculation to birthing... In my own head, I believe that keeping them untaped while they are still feeble and prone to disease, would in fact, introduce disease. At 1/2 colonization, the cake should be healthy enough to ward off any contaminents entering through the needle holes and vermiculite casing layer, right? Also, the tape needs to be off at this point to let CO2 exchange occur, correct?

Thank you for all your answers!
kspoker


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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Possible contamination questions... first time [Re: kspoker]
    #1144272 - 12/15/02 09:23 PM (14 years, 8 months ago)

The BRF with little chunks of unground rice is probably even better for the mycelium...


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InvisiblePsychoslut
The Mother Fucking Bear-o-dactyl
 User Gallery
Registered: 12/10/02
Posts: 20,807
Loc: all up in ya
Re: Possible contamination questions... first time [Re: kspoker]
    #1144416 - 12/16/02 04:44 AM (14 years, 8 months ago)

I used a normal margarita blender and put brown rice in there and blended it up withouth any liquid of course, it made it into a powder with very small sand-like chunks, try that you can prolly get it more flowery like how i did


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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