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Re: A promising means to supercharge potency? [Re: FooMan]
    #4553608 -

Thank you for the compliment.

Yeah, scat and I bumped heads...i guess he thought I cared about my rating or something. I've been known to rub people the wrong way...hell, who am I kidding... i'm famous for it... :wink:

EDIT: I used less than 1cc per jar. But I did not tamp them, which I believe leaves more air and speeds up colonization.


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Diabetes causes hamsters.

Edited by Bamaman (08/18/05 11:43 PM)

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Re: A promising means to supercharge potency? [Re: Bamaman]
    #4553677 -

Bamaman said:
I did not tamp them, which I believe leaves more air and speeds up colonization.



Definitely speeds colonization!

I had a jar one time that I never inoculated and forgot about. It started growing what looked like myc, but instead of growing from one point outward, it started growing in a bunch of different little spots. It was some sort of mold. Had me thinking that I was going crazy! Here's a pic:



Notice how the "myc" seems to be growing in a bunch of different spots? True myc usually grows from the inoculation points outward like this:


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Quick WBS Prep

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Re: A promising means to supercharge potency? [Re: FooMan]
    #4553834 -

Seems there is a great deal of nonuniformity in the melatonin sources we've been procuring. The bottle I got and recommend as the best for this use is as follows.

"Nature's Bounty
Melatonin 3mg
Nighttime Sleep Aid
Dietary Supplement
"

Complete list of ingredients in a Nature's Bounty tablet:

Melatonin (3mg per tablet)

Dicalcium phosphate
(definitely safe for substrates)

Cellulose
(definitely safe, of plant origin)

Vegetable Magnesium Stearate
(safe according to my research, it's a hydrophobic (water repelling) neutral magnesium compound, so I doubt it'd be absorbed that readily anyways)

Vegetable stearic acid
(safe, is merely a simple saturated fatty acid)

Silica
(definitely safe, the oxide of silicon; formula SiO2)

Croscarmellose
(safe from what I have read, it's a stable hygroscopic (moisture absorbing) sodium compound)

I recommend this brand because 1) you won't find too many unnecessaries in it, 2) what it does have I would consider safe from what I have read, and 3) it has the most melatonin per tablet. Reason I have number 3 is because I'm pretty damn suprised that Bamaman has such a low potency tablet.

As for the question of if a 3mg tablet is "too low" to be considered worth pursuing:

Ramuh said:
Man... .3mg a pill? Ill have to start looking at health / vitamin sellers around here to see what kinda stuff they sell. This is ALMOST getting to the point where I would say its just not worth it :P. If it takes that many pills per jar, that would get pretty expensive just to raise potency.



Psilocybin Dosages
(Reference: http://www.erowid.org/plants/mushrooms/mushrooms_dose.shtml)

Code:
Threshold   2 mg
Light 2 - 4 mg
Common 4 - 8 mg
Strong 8 - 20 mg
Heavy 20 + mg



It should be noted that the active dosages for psilocybin surely aren't large either. I still think it is wholly possible that a relatively sizable quantity of melatonin tablets added to substrate could possibly increase potency, because it seems that the mgs are relative.

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Re: A promising means to supercharge potency? [Re: squeedgie]
    #4553857 -

Oh...my psilocybin doses were off in my head then :P.

Count me back in the train of testing then! My syringe should probably arrive tuesday or so, and Ill probably do a LC.


--------------------
Radio is down for a day or two to transfer all my music, and do some other work.

"Light, coming from my mind. We are one, the night has just begun"

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Re: A promising means to supercharge potency? [Re: Ramuh]
    #4554298 -

I dont know if this has been discussed already but has anyone looked into grinding 5-HTP(5 hydroxytryptophan) tablets into powder and adding that to the substrate?? It seems like a very direct source of tryptophan, but i dont have an old bottle on hand to check how much tryptophan they contain. I guess if it hasnt been tried ill try it on some new jars once my outdoor flush beginss friuting. I know the pills alone DEFENITELY imporved my mood so there must be enough tryptophan in there.

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Re: A promising means to supercharge potency? [Re: droptopsunfire03]
    #4554375 -

It was touched upon. To recount what was said:

5-HTP is a precursor to tryptophan, which is a precursor to tryptamine, which is a precursor to the higher tryptamines including serotonin, DMT, psilocin, psilocybin, and melatonin (not necessarily in that order).

Might want to take a glance at http://deoxy.org/trypgif.htm.

If you're looking for tryptophan you'd do better using quinoa grains in your substrate because they contain a large amount of naturally occurring tryptophan.

5-HT is legal and one could conceivably do that, but it wouldn't be cost-effective whatsoever. The purpose of this experiment is to see if melatonin (which is a "higher" tryptamine) can be metabolized by the fungi to produce a higher potency product.

Edited by squeedgie (08/19/05 03:43 AM)

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Re: A promising means to supercharge potency? [Re: squeedgie]
    #4566281 -

8/18/05
White dots getting bigger, resembling white rice particles or white sand granules stuck together.

8/19/05
White dots becoming much more abundant causing the substrate to look like it was mixed with a lot of white sand.

8/20/05
White dots now more dominant in appearance than the substrate itself, one jar contains a bright white mycelia growth.

8/21/05 - 11:00am(5 and a half days after inoculation)
Approx. 9 out of the 12 jars contain patches of bright white mycelia. Some jars just a single patch others with 2, 3, and 4 patches.




--------------------
Diabetes causes hamsters.

Edited by Bamaman (08/22/05 01:39 PM)

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Re: A promising means to supercharge potency? [Re: Bamaman]
    #4570024 -

Excellent how they are coming along so far. Nice job with the elimination of the glare.

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Re: A promising means to supercharge potency? [Re: squeedgie]
    #4570052 -

I still haven't gotten a shipment notification from sporeworks yet (Ive had good experiences with them in the past though, so its cool). I'll be sending them an email later today if I still don't get one, its almost been a week since I placed the order. Last time I got shipment notice 23 hours after I placed my order :P.


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Radio is down for a day or two to transfer all my music, and do some other work.

"Light, coming from my mind. We are one, the night has just begun"

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Re: A promising means to supercharge potency? [Re: squeedgie]
    #4570325 -

Quote:
5-HTP is a precursor to tryptophan, which is a precursor to tryptamine, which is a precursor to the higher tryptamines including serotonin, DMT, psilocin, psilocybin, and melatonin (not necessarily in that order).



You sure about that? From what i gather in human body at least the pathway goes like tryptophan->5HTP->serotonine ,the limiting step is the one convertion tryptophan to 5HTP ,hence giving 5HTP you greatly increase serotonine,moreso than giving tryptophan....

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Re: A promising means to supercharge potency? [Re: Psiloman]
    #4571570 -

8/22/05 - 8:00am(6 and a half days after inoculation)
11 out of the 12 jars contain patches of bright white mycelia. Some jars just a single patch others with 2, 3, and 4 patches.


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Diabetes causes hamsters.

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Re: A promising means to supercharge potency? [Re: Bamaman]
    #4572513 -

RE:
http://www.shroomery.org/forums/showflat.php/Number/2484390#Post2484390

Trying to get the numbers right to get an idea of how much my cakes total yield will be.

Based on the GT brf cakes in the above link's grow log...

....approx. 6 grams dry per cake, per flush. His first flush averaged 10+ dry grams, but he says he never gets less than 6, so conservatively I'll go with 6.

Also, I've heard tell that a properly done cake can get 3, 4, or even 5 flushes. Again, conservatively I'll just say 3 flushes. At 12 cakes and 3 flushes per cake,(6grams x12cakes x3flushes), comes to 216 dry grams.

Q.#1Is this about what I can expect to get?? (Obviously, assumming I don't lose any to contams)

In the past(15yrs ago or so) I got wild shrooms regularly that were out of a cow field(southeast georgia, cubes presumably).

Q. #2 Will my GT brf/quinoa produce more potent shrooms than the average wild cube, or weaker?

Q.#3 Either way, what is the approximate dosage for a kick ass trip with my particular shroom/substrate combo.

Thanks in advance.


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Diabetes causes hamsters.

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Re: A promising means to supercharge potency? [Re: Bamaman]
    #4572831 -

Just finally got the notice that sporeworks is temporarily taking a break. SO, tomorrow Itll get shipped, and hopefully it will arrive by friday! I want to get this thing started after all this waiting wondering why nothing was being shipped :P.


--------------------
Radio is down for a day or two to transfer all my music, and do some other work.

"Light, coming from my mind. We are one, the night has just begun"

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Re: A promising means to supercharge potency? [Re: Ramuh]
    #4580188 -

2/25/05 - 4:00pm (a little less than 9 days after inoculation)
All jars have bright white mycelial growth. 1 jar has just a tiny bit, probably not noticeable in the pic. Another 1 has very little in comparison with the rest.



Keep in mind that there is growth in many of these jars which is on the side not facing the camera. Also know that the last 2 days the temp has only read about 78. I had a cord malfunction that I found when I checked the temp yesterday, thought I had fixed it only to find the temp still low today...grrr. It is now fixed and my incubator temp is rising to normal again.


--------------------
Diabetes causes hamsters.

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Re: A promising means to supercharge potency? [Re: Bamaman]
    #4581580 -

Finally have a confirmed shipping date. My spore arrive Monday ><.

What temp are you incubating at Bamaman?


--------------------
Radio is down for a day or two to transfer all my music, and do some other work.

"Light, coming from my mind. We are one, the night has just begun"

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Re: A promising means to supercharge potency? [Re: Ramuh]
    #4581616 -

82-83


--------------------
Diabetes causes hamsters.

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Re: A promising means to supercharge potency? [Re: Bamaman]
    #4581630 -

sound good, good luck you guys! this is really interesting stuff


--------------------
Hail to the King Baby

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Re: A promising means to supercharge potency? [Re: AsianYumYum]
    #4584653 -

Well, I got pleasantly suprised today when my spores arrived today instead of the scheduled date of monday ^^.

Ill start up my LC tonight! Hope to be right up there with you soon Bamaman :wink:.


--------------------
Radio is down for a day or two to transfer all my music, and do some other work.

"Light, coming from my mind. We are one, the night has just begun"

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Re: A promising means to supercharge potency? [Re: Bamaman]
    #4590599 -

8/28/05 - (11 and a half days after inoculation)



--------------------
Diabetes causes hamsters.

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Re: A promising means to supercharge potency? [Re: Bamaman]
    #4590668 -

is it me or are these colonizing slowly?

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