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Offlinefoo
tsonoqua
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Registered: 08/21/01
Posts: 101
Last seen: 18 years, 1 month
Glove Box
    #455609 - 11/12/01 12:34 AM (22 years, 8 months ago)

I don't understand. Jars can't be filled and closed and sterilised and innoculated and... .inside a glove box so how do they make any difference?? what CAN you do in them other than innoculation.
Is there anywhere I can read about glove boxes??


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"I get up, I walk, I fall down. Meanwhile, I keep dancing."

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Offlinemoxjet20
member
Registered: 07/25/01
Posts: 160
Loc: santa cruz, magical land ...
Last seen: 22 years, 1 month
Re: Glove Box [Re: foo]
    #455613 - 11/12/01 12:41 AM (22 years, 8 months ago)

well, actually you probably could fill, close, sterilize, etc.. your jars in a glove box, there is no point however since you are going to sterilize it at a later point anyways. from what i understand the main problem with contams however IS at innoculation, that's why they are so good. however if you're doin pf tek then i don't see the need for 'em, 1/2" of vermiculite on the top does the trick. i think gloveboxes are used more with agar culture techniques, where trying to be neat and not coughing on shit just doesn't cut it.


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OfflineNewbieShroomer
member

Registered: 10/01/01
Posts: 199
Loc: San Diego California Baby...
Last seen: 22 years, 6 months
Re: Glove Box [Re: foo]
    #455939 - 11/12/01 12:01 PM (22 years, 8 months ago)

I am building a glovebox, am starting a new thread on it's uses tho, will be up today :)


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OfflineGatorade
A-With-A-Teeth-A

Registered: 05/17/05
Posts: 175
Last seen: 15 years, 9 months
Re: Glove Box [Re: NewbieShroomer]
    #4227097 - 05/27/05 03:07 PM (19 years, 1 month ago)

I too made a glove box... it helps prevent germs from floating around and landing on the syringe.

In rooms that are dirty or have running central air, they are most useful.


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OfflineHippieChick
Chicks can do it too!
Female User Gallery

Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 6 months
Trusted Cultivator
Re: Glove Box [Re: Gatorade]
    #4227116 - 05/27/05 03:17 PM (19 years, 1 month ago)

They are really needed for G2G transfers. Which after seeing the results of my attempt, would reccommend G2G for everyone. Turned two quarts into 40 in 8 days.
Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


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Offlineyumyum
Stranger
Registered: 08/23/04
Posts: 34
Last seen: 15 years, 4 months
Re: Glove Box [Re: HippieChick]
    #4227183 - 05/27/05 03:43 PM (19 years, 1 month ago)

glove boxes really aren't used for innoculating jars. that is the whole purpose of jars is to elimiate the need for a glove box. the maine pupose of a glove box is for doing things like G2Gs, printing, cloning, and agar work. but actually a "glove box isn't really needed, if you design your air flow to come from the top, just make it a flow hood and all is good. =) few bucks cheaper to.

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InvisibleLiquidkick
H2O
Registered: 05/03/02
Posts: 2,635
Re: Glove Box [Re: yumyum]
    #4227208 - 05/27/05 03:53 PM (19 years, 1 month ago)

I didn't use a glove box or anything and did it in open air a g2g, wish me luck.

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Offlineyumyum
Stranger
Registered: 08/23/04
Posts: 34
Last seen: 15 years, 4 months
Re: Glove Box [Re: Liquidkick]
    #4227214 - 05/27/05 03:58 PM (19 years, 1 month ago)

if you do it quick enough, without much hesitation, you should be fine, unless you where doing it in a mildew infested basement or something.

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