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 MirrorImage
newbie
Registered: 03/19/04
Posts: 50
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 Glove box 
#2790335 - 06/13/04 04:40 PM (20 years, 4 months ago) |
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been using a home made glovebox for a while now & am having great success with it. only thing is, that it is a pain in the ass to set up every time. so, as i came into some money recently, i thought i would invest in a decent glovebox. looking through the web, i spotted this one by
myco supply.
just wondering if anyone knows if there are any better ones than this out there?
cheers! 
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 FallenShroom
Shagadelic
Registered: 05/01/03
Posts: 879
Last seen: 16 years, 6 months
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lot of money for pvc and clear plastic 289.00 wow
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Citric
Registered: 03/19/04
Posts: 4,490
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IfIWereARichMan
journeyman
Registered: 10/27/03
Posts: 84
Last seen: 13 years, 3 months
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Re: Glove box [Re: Citric]
#2790440 - 06/13/04 05:30 PM (20 years, 4 months ago) |
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Get a flowhood instead. Much less restrictive.
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Rat
Stranger
Registered: 04/20/04
Posts: 103
Last seen: 19 years, 2 months
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My glove box cost $6 and it probably just as effective as the $290 model that myco supply has. Is the pain that much that you'd rather put down that kid of money for something that can't be much more complex than what you have.
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berto23
Stranger
Registered: 04/26/04
Posts: 250
Loc: NJ
Last seen: 11 years, 7 months
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lol i love how he said "came into money!"
what a great hobby this is
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the shnozzberrys taste like shnozzberrys
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TripleB
Hobbyist
Registered: 04/01/04
Posts: 1,358
Loc: Michigan
Last seen: 10 years, 2 months
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there has to be something up with that glovebox... HEY MYCOSUPPLY DUDE, LET US IN ON WHY ITS SO EXPENSIVE!
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MirrorImage
newbie
Registered: 03/19/04
Posts: 50
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Re: Glove box [Re: berto23]
#2790848 - 06/13/04 08:27 PM (20 years, 4 months ago) |
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i've already gone down the route of making a glove box cheap - it works ok - but quite frankly could be better. i can't be ringed to make a more effective one, as i am rather short on time. & time is money - so am willing to make an investment that will give me the guarenteed sterility for doing work with agar, etc. something my current glove box does not do. i do take your points on board though. the reason i asked the question in the first place was because i wanted to know if there was a better one (than the one mycosupply offers)that anyone knew off. maybe i will look into the flowhood idea though. thanks for the suggestion.
pics of my current glovebox - making prints:
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QandA
wanderer
Registered: 05/19/04
Posts: 352
Last seen: 20 years, 2 months
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Nothing is guaranteed sterile. If you are still having contam problems with the glovebox you've got now, I bet it's something else in your procedure, not with your glovebox. Down with the 290.00 glovebox. You are basically throwing your $$$$ away. Invest it in something worthwhile. Just my 2 shits.
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I'm not crazy...crazy.
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psilomonkey
Twisted brainwrong of a oneoff man mental
Registered: 08/08/03
Posts: 812
Loc: Airstrip One
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I have got by fine with a clear plastic bin and a roll of cling film. Just lay the box on its side, pull the cling film tight over the front. Leaving a gap at the bottom to put your hands under.
Avoid drafts, and spray around inside with some anti-bact.
A glovebox is not a substitute for good aseptic technique.
Have everything you need inside before you start, that way you are not going outside your glovebox and picking up contams. Have lots of Isopropyl Alcohol to clean stuff. I have done dozens of grain to grains in this setup, so for no contams.
The bin with its lid is also a good place to let things cool down after PCing, I think my contams of old were due to letting the jars cool down in a contam rich environment, as the jars cooled they drew in contams.
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Nexxiss
Mushroom Mage
Registered: 05/07/04
Posts: 109
Loc: Mich,USA
Last seen: 18 years, 6 months
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well , i dont want to judge your construction, but the teks here work wonders. i know its a pain to search...<pretend i have never posted without searching:)>but this dogs been beat to death a couple times. i have a tub with a comp fan mounted on the side , i think is a tek here.
as of late i am getting lasy <thx magash  .i dont turn fan on,sometimes use gloves sometimes not, but ill say this i am very sterile. wipe everything down before,dont touch anything with needle but what im injecting.i just pretent im working in a lab with hazardous chems. so far i get no contams either way.
Nex
if you follow Magash, shrooms will come!
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Nexxiss Reign
"as I looked into the Sun and realized the race is not to the swift nor Knowlege to the wise ..nee the yet strenth to the strong...nor Riches to the poor.But time and Chance hapenth to us all!
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MirrorImage
newbie
Registered: 03/19/04
Posts: 50
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Re: Glove box [Re: Nexxiss]
#2792584 - 06/14/04 01:55 PM (20 years, 4 months ago) |
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*sigh* just a few points - first of all - i haven't really had any contams with my homemade glovebox. just the one out of maybe 20 times using it. secondly, i wanted to invest in a proffesional setup. i want features like pass through, possitive pressure - hopefully with an isolation chamber. this will come in incredibly useful when working with agar & general strain isolation. having looked at the responses though, i've decided to go about making a flow hood. thankfully i have a m8 who is good with practical things, & is willing to help me sort out the build. thanks for the help everyone - you helped me reach a decision
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psilomonkey
Twisted brainwrong of a oneoff man mental
Registered: 08/08/03
Posts: 812
Loc: Airstrip One
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I think you will be better off accepting that some will fail. 1:10 is perfectly acceptable to the home grower IMO.
You say 1:20, I would say your technique is well on form!
If you are going to be cloning from live tissue, peroxide/glovebox works well enough for me, sterile areas are needed when isolating spores from the wild. I would like to play, but can't afford the microscope right now.
Having said that, I am planning building a flowhood, cos I am a enthusiastic muppet 
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MirrorImage
newbie
Registered: 03/19/04
Posts: 50
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well said psilomonkey! i guess the biggest problem with the whole glovebox thing is the limited space. i guess if you are innoculating a few jars the glovebox does quite well. however if you gotta do mycobags & jars - it all gets very traumatic (see above pics for spore printing hell). flowhood does seem to be the way forward - already reading up on it.
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doomsdayrobert
Stranger
Registered: 09/06/04
Posts: 118
Last seen: 6 years, 3 months
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The glove box looks nice, but now it runs for $350! I couldn't imagine
paying such a price...You should be able to find a decent glovebox for quite a bit cheaper. If you have the money and don't mind spending it, then go for it.
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Darkie
Bitches n hoes dont mean a thing
Registered: 01/22/05
Posts: 216
Last seen: 12 years, 2 months
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The way i look at it is if you can make one as good as that one in say....4 hours for $20, youre getting paid about 60$ an hour....DAMN! I wish i could find a job like that. A couple nice features you could easily add are the positive pressure thing and a double door entry chamber for putting shit in there easily and constantly. Maybe even incorperate a UV light to make a decontam air lock chamber....oohhh ahhh....
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You gotta wake up to get faded but you gotta get up to get paided.
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Hotnuts
old hand
Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 1 month, 2 days
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Re: Glove box [Re: Darkie]
#3925063 - 03/16/05 11:01 AM (19 years, 7 months ago) |
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Yeah man. If you're going to spend a nice chunk of money, let's say $250 plus. You should check out flowhoods for sure. There so much easier to do work with. Check ebay. Every now and then one comes up there for auction, or i'm sure someone here might have a link up their sleave.
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc: 
Last seen: 11 years, 9 months
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Re: Glove box [Re: Hotnuts]
#3925080 - 03/16/05 11:08 AM (19 years, 7 months ago) |
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PM agar, he has plans for an excellent pro flowhood...it was either 150 or 250 or something...BIG arsed thing though...
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Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
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Fuck that glove box man, mine cost 10 dollars and It works perfeclty.
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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
Edited by Psychoslut (03/16/05 11:11 AM)
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doomsdayrobert
Stranger
Registered: 09/06/04
Posts: 118
Last seen: 6 years, 3 months
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Quote:
Psychoslut said:
Fuck that glove box man, mine cost 10 dollars and It works perfeclty.
Yeah, I made a glove box out of an empty case of beer, a plastic drop cloth, rubber gloves, tape and scissors. It cost abou $4 for some nice dishwashing gloves...
It's not the best, but if you wipe the inside with lysol it won't fail 
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