liquid medium w/malt extract--how much?

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Phogger
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Registered: 09/25/01
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liquid medium w/malt extract--how much?

#442573 - 10/30/01 10:41 PM (24 years, 9 months ago)

I'm going to try the liquid medium mycelium growth tek described in this site. It calls for 4% malt extract by weight to distilled water. Since I don't have a scale, I'm wondering if anyone has tried this using malt powder and how many tsps/tbls they used per 1/2 qt. jar. Thanks.

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Shdwstr
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Registered: 02/17/01
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Loc: Ontario, Canada
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Re: liquid medium w/malt extract--how much?

[Re: Phogger]
#442600 - 10/30/01 11:14 PM (24 years, 9 months ago)

Malt powder weighs approx. 10 gr / tbsp. A 4% solution would be 1 tbls. / 250 ml (1 cup) of water.

Shdwstr

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Phogger
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Re: liquid medium w/malt extract--how much?

[Re: Shdwstr]
#442644 - 10/30/01 11:59 PM (24 years, 9 months ago)

Thanks for your help, Shdwstr. That's what I needed to know.

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zhukov
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Re: liquid medium w/malt extract--how much?

[Re: Phogger]
#442649 - 10/31/01 12:07 AM (24 years, 9 months ago)

Yep, 4% at that ratio is ok :wink:

- Good luck Phogger! - I reckon that malt powder is better than honey; I might be wrong but it seems to be more nutrient-rich...don't worry if it turns a much darker colour than honey, it's normal...:)

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rasputinn
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Re: liquid medium w/malt extract--how much?

[Re: zhukov]
#16086058 - 04/13/12 05:33 PM (14 years, 4 months ago)

well this is what i get with 20 gr lme 7 gr yeast(some fucker on here gave me this recipe)my scale goes to a 1000th of a scale

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rasputinn
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Re: liquid medium w/malt extract--how much?

[Re: rasputinn]
#16086060 - 04/13/12 05:34 PM (14 years, 4 months ago)

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Riseup2day
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Re: liquid medium w/malt extract--how much?

[Re: rasputinn]
#16087099 - 04/13/12 09:51 PM (14 years, 4 months ago)

use a coffee filter and strain the liquid, it helps a little.

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Luger0815
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Re: liquid medium w/malt extract--how much?

[Re: Riseup2day]
#16087561 - 04/14/12 12:25 AM (14 years, 4 months ago)

Malt Extract Agar (3%)(MEA)(General Microbiology)(FDA M93)
-----------------------------------------------------------------
30 g Malt extract (liquid) or 20 g Dry Malt Extract
20 g Agar
1 L Distilled water

Boil to dissolve ingredients. Avoid overheating, which causes softening of agar and darkening of medium color. Autoclave 15 min at 121?C. Dispense 20-25 ml into sterile 15 x 100 mm petri dishes. Final pH, 5.5 ? 0.2.

This medium is recommended as a general maintenance medium. [1]

For LC just let the Agar out...

Fast Fred's Cookbook

Edited by Luger0815 (04/14/12 12:26 AM)

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Luger0815
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Re: liquid medium w/malt extract--how much?

[Re: rasputinn]
#16087578 - 04/14/12 12:30 AM (14 years, 4 months ago)

rasputinn said:

well this is what i get with 20 gr lme 7 gr yeast(some fucker on here gave me this recipe)my scale goes to a 1000th of a scale

2-3g dry yeast is enough.
Filter the LC trough 2 coffee filter before you sterilize it.
It wont harm if you dont filter it and it will also work with 7g yeast but it is not clear then.

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