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Amazon Shop for: ½ Pint Jars, Agar, Autoclave, Malt Extract, Petri Dish, Scales, pH Test Strips

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OfflinePhogger
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liquid medium w/malt extract--how much?
    #442573 - 10/31/01 12:41 AM (15 years, 1 month ago)

I'm going to try the liquid medium mycelium growth tek described in this site. It calls for 4% malt extract by weight to distilled water. Since I don't have a scale, I'm wondering if anyone has tried this using malt powder and how many tsps/tbls they used per 1/2 qt. jar. Thanks.


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OfflineShdwstr
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Re: liquid medium w/malt extract--how much? [Re: Phogger]
    #442600 - 10/31/01 01:14 AM (15 years, 1 month ago)

Malt powder weighs approx. 10 gr / tbsp. A 4% solution would be 1 tbls. / 250 ml (1 cup) of water.

Shdwstr


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OfflinePhogger
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Re: liquid medium w/malt extract--how much? [Re: Shdwstr]
    #442644 - 10/31/01 01:59 AM (15 years, 1 month ago)

Thanks for your help, Shdwstr. That's what I needed to know.


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Offlinezhukov
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Re: liquid medium w/malt extract--how much? [Re: Phogger]
    #442649 - 10/31/01 02:07 AM (15 years, 1 month ago)

Yep, 4% at that ratio is ok :wink: - Good luck Phogger! -  I reckon that malt powder is better than honey; I might be wrong but it seems to be more nutrient-rich...don't worry if it turns a much darker colour than honey, it's normal...:)


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Offlinerasputinn
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Re: liquid medium w/malt extract--how much? [Re: zhukov]
    #16086058 - 04/13/12 07:33 PM (4 years, 7 months ago)

well this is what i get with 20 gr lme 7 gr yeast(some fucker on here gave me this recipe)my scale goes to a 1000th of a scale


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Offlinerasputinn
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Re: liquid medium w/malt extract--how much? [Re: rasputinn]
    #16086060 - 04/13/12 07:34 PM (4 years, 7 months ago)



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OfflineRiseup2day
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Re: liquid medium w/malt extract--how much? [Re: rasputinn]
    #16087099 - 04/13/12 11:51 PM (4 years, 7 months ago)

use a coffee filter and strain the liquid, it helps a little.


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InvisibleLuger0815
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Re: liquid medium w/malt extract--how much? [Re: Riseup2day]
    #16087561 - 04/14/12 02:25 AM (4 years, 7 months ago)

Malt Extract Agar (3%)(MEA)(General Microbiology)(FDA M93)
-----------------------------------------------------------------
30 g Malt extract (liquid) or 20 g Dry Malt Extract
20 g Agar
1 L Distilled water

Boil to dissolve ingredients. Avoid overheating, which causes softening of agar and darkening of medium color. Autoclave 15 min at 121?C. Dispense 20-25 ml into sterile 15 x 100 mm petri dishes. Final pH, 5.5 ? 0.2.

This medium is recommended as a general maintenance medium. [1]

For LC just let the Agar out...

Fast Fred's Cookbook


Edited by Luger0815 (04/14/12 02:26 AM)


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InvisibleLuger0815
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Re: liquid medium w/malt extract--how much? [Re: rasputinn]
    #16087578 - 04/14/12 02:30 AM (4 years, 7 months ago)

Quote:

rasputinn said:
well this is what i get with 20 gr lme 7 gr yeast(some fucker on here gave me this recipe)my scale goes to a 1000th of a scale





2-3g dry yeast is enough.
Filter the LC trough 2 coffee filter before you sterilize it.
It wont harm if you dont filter it and it will also work with 7g yeast but it is not clear then.


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Amazon Shop for: ½ Pint Jars, Agar, Autoclave, Malt Extract, Petri Dish, Scales, pH Test Strips

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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