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OfflineRandolph_Carter
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Alternate liquid mediums
    #422381 - 10/11/01 09:52 PM (15 years, 4 months ago)

intrest+time=results.
The sugar content of a usual medium is either regulated (say, malt extract, other pure forms of sugar) or known, as in the case of honey. Honey has a sugar content composed of approximately 39% fructose, 32% glucose, and 2% sucrose, with the rest composed of water and a few other things (1). So, with this in mind, i went out to find a few things resembling it...that i can easily get to that is.
1: Apple juice: 98% soluable items are sugars, with the main constituents being fructose and glucose in a ratio of approx 2/1....who wants to grow some apple shrooms? (2)
2: High frustose corn syrup: 55% fructose, 40% glucose (2)
Sooo...with this in mind, have fun and let me know if it works.
1: http://www.inetone.net/mshapiro/honey.html
2: http://www.chem.agilent.com/cag/peak/peak2-97/article1.html

stay frosty yall.




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(This has been a +1 production.)


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Offlineduncan8r
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Re: Alternate liquid mediums [Re: Randolph_Carter]
    #423683 - 10/12/01 09:25 PM (15 years, 4 months ago)

MokEY uses a kit called AUsTops to convert fruit juice to sparklin alco juice. The non refrigerated bottled juice on the supermarket shelf works as it has no preservatives.

It might be worth a go, but Im not too sure of the pH of these juices?? anyone help.... I like the thought of monkey making 2 litres of apple mycelium juice though.

Duncan8r and his latex ,lickin monkey

Not all chemicals are bad. Without chemicals such as hydrogen and oxygen, for example, there would be no way to make water, a vital ingredient in beer. --Dave Barry


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Not all chemicals are bad. Without chemicals such as hydrogen and oxygen, for example, there would be no way to make water, a vital ingredient in beer. --Dave Barry


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Offlinegusb232
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Re: Alternate liquid mediums [Re: Randolph_Carter]
    #423863 - 10/12/01 08:57 AM (15 years, 4 months ago)

Apple juice seems like it could be the next liquid craze, All i need to know is what is the total percent of dissoved sugers in juice to know how much to dillute it if any. If it was lets say around 10% ( which i understand if favorable to mycleial growth) then all one would have to do is inject a syringe directly into and unopend bottle and in a few weeks you'd have a gallon of mycleil water Wow!. that sounds incredible to me.
im sure some one will try this, please post results if anyone does.
very cool!

The most incomprehensible thing about the universe is that it is comprehensible.
Eistein


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Offlineblackout
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Re: Alternate liquid mediums [Re: gusb232]
    #423923 - 10/12/01 01:19 PM (15 years, 4 months ago)

apple juice is normally between 9 and 11% sugar. you want a solution of 4% sugar (not 10%) so it will have to be diluted. organic apple juice is easy to get these days and not to expensive


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Offlinesylo
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Re: Alternate liquid mediums [Re: Randolph_Carter]
    #424776 - 10/14/01 03:51 AM (15 years, 4 months ago)

There is a tek for growing shroom mycelium in large jugs of liquid media called the Szuecs Tek (pronounced Sooch). The Szuecs setup uses a glass jug with a two hole stopper. A glass rod passes through one hole of the stopper to the bottom of the jug while the other hole serves as an air vent and inoculation port. A plastic tube for air is attached to the glass rod at the top of the jug and extends outside the jug to a thick glass tube filled with cotton or polyfill that acts as an air filter. A fish tank bubbler forces air through the filter and down the glass tube to the bottom of the jug. Just like a fish tank. Air bubbling up through the medium provides both aeration and agitation. With a high rate of air flow the mycelium grows as compact pellets but with a low rate of flow the mycelium is filiamentous. The jug is filled about two thirds of the way to the top with a liquid medium and the entire apparatus, except for the bubbler, is autoclaved as a unit. The Szuecs Tek has been mostly used for growing shrooms that are not easily cultivated such as Morchella (morels) and the dried mycelium can be used as a soup ingredient. This is a difficult tek to use because it is highly prone to contams and requires a large autoclave for sterilization.

Some odd ingredients that have been used for liquid culture are: soybean whey, beet molasses, cheese whey, corn and pumpkin canning waste, and (for wood lovers) the sulfite waste from paper manufacturing.


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Offlineblackout
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Re: Alternate liquid mediums [Re: sylo]
    #424940 - 10/14/01 10:57 AM (15 years, 4 months ago)

that is a very interesting tek. i did a search for it and it has not been mentioned before. i searched in google and found this.

http://www.fransdijkstra.nl/diss/chapter5.htm

they talk about growing in 10 litre containers with glucose, skim milk powder and meat extract. they grew edible mushroom mycelium for their taste.
here is an interesting quote from the page.

"The agitation of the medium greatly influenced the results of the experiments. With Coprinus comatus the highest yields of mycelium were obtained with a stirring rate of 450 rev/min. Agaricus bisporus grew better at 225 rev/min than at 450 rev/min, giving a higher maximum yield of mycelium while the time required to obtain this yield was shorter. It is interesting, that growth in fermentors was considerably faster than in shaken flasks, where 2 to 3 weeks were necessary to reach the maximum yield of mycelium."
it only took one week in the fermentor


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Offlinesylo
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Re: Alternate liquid mediums [Re: blackout]
    #426033 - 10/15/01 04:24 AM (15 years, 4 months ago)

My info on the Szuecs Tek is sketchy but he did get patents on it and there should be more information with his patent descriptions.
Szuecs, J., U.S. Patent 2,693,664 (Nov 9, 1954)
Patent 2,761,246 (Sept 4, 1956), and 2,850,841 (Sept 9,1958)
Unfortunately, on line patent searches don't go back that far.


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Offlineblackout
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Re: Alternate liquid mediums [Re: sylo]
    #426325 - 10/15/01 02:50 PM (15 years, 4 months ago)

you could fit a 2 gallon glass wine fermentor in an oven and blast it for 2 hours. the guy who wrote the thesis also talks about great success growing using vegetable oil and even grows in 2% alcohol with good results


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Offlinegusb232
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Re: Alternate liquid mediums [Re: blackout]
    #426347 - 10/15/01 03:06 PM (15 years, 4 months ago)

4 % huh, that could explain my unsuccessfully atempts at liquid medium. I dont measure too exactly but, i have used around ten percent honney to water, it usualy just grows really slow. Does this 4% also apply to starches like potato water?
thanks


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Invisiblepuscle
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Registered: 01/07/01
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Re: Alternate liquid mediums [Re: gusb232]
    #426360 - 10/15/01 03:18 PM (15 years, 4 months ago)

At 10% solution, sugars become toxic to mycelia. A range of 4 - 6% is fine. Yes, this includes dextrose.


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Offlineblackout
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Re: Alternate liquid mediums [Re: puscle]
    #427310 - 10/16/01 02:09 PM (15 years, 4 months ago)

"Does this 4% also apply to starches like potato water? "
i dont know but if it does but you will have to find out how much carbohydrate is in the potato. it grows on rice without "dilution" though....
it will grow fine as long as it 4% or below. but if it is too weak it is more open to other bacteria (which wont tolerate a 4% solution)


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