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Offlineready2go
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Registered: 07/19/05
Posts: 249
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Last seen: 12 years, 7 months
Liquid medium, serilization in the oven?
    #5027366 - 12/07/05 05:58 AM (18 years, 2 months ago)

All right I'm trying to play around with agar and liquid midiums to grow batches of myc. My problem is i have a small PC and cant quite fit qt jars in an upright possition.... this is fine for my grains I just PC them laying on thier side.
Can I put my jars in the oven @ 200 for 30-60 min or so to sterilize? I use oven tec to serilize and pasturize my casing and buld substraits mixutures and have had great results.
Now i know moisture loss is a issue so i figure i can put the jars in a oven bag with water in the bottom and sealed. keeps my chicken from drying out. Think this might work? any input would be greatly apreciated.
ready


Edited by ready2go (12/07/05 07:54 AM)


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Offlinexburn
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Registered: 03/10/05
Posts: 707
Last seen: 11 years, 7 months
Re: Liquid medium, serilization in the oven? [Re: ready2go]
    #5027526 - 12/07/05 08:31 AM (18 years, 2 months ago)

one problem is it takes the medium much longer to heat to the 200 degrees than if it where in a pressure cooker. If you do do it this way spray the oven sterile. I have used a microwave with good sucess in the past for LC i usually did 7 min stop wait 2 repeat 3-4 times


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Invisibletiny_rabid_birds
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Registered: 11/08/05
Posts: 15,653
Loc: estados unidos
Re: Liquid medium, serilization in the oven? [Re: xburn]
    #5031998 - 12/07/05 11:52 PM (18 years, 2 months ago)

Depending on whether your jar lids are metal or plastic and the size of your microwave, you might be able to do what this tek http://www.shroomery.org/index.php/par/24254 advises, and sterilize your jars and medium using the microwave, as quoted below.

"Karo sterilization:

Note: Clean the microwave out. Clean it as well as possible with bleach.

Place the jar into your microwave. Set the heat setting on high. Cook it for 1 minute. Wait 5 minutes and cook for another minute. CAUTION THE JAR WILL BE HOT! Leave it in the Microwave over night. This will keep it clean as it cools. "


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