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Liquid medium, serilization in the oven?
    #5027366 -

All right I'm trying to play around with agar and liquid midiums to grow batches of myc. My problem is i have a small PC and cant quite fit qt jars in an upright possition.... this is fine for my grains I just PC them laying on thier side.
Can I put my jars in the oven @ 200 for 30-60 min or so to sterilize? I use oven tec to serilize and pasturize my casing and buld substraits mixutures and have had great results.
Now i know moisture loss is a issue so i figure i can put the jars in a oven bag with water in the bottom and sealed. keeps my chicken from drying out. Think this might work? any input would be greatly apreciated.
ready

Edited by ready2go (12/07/05 07:54 AM)

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Re: Liquid medium, serilization in the oven? [Re: ready2go]
    #5027526 -

one problem is it takes the medium much longer to heat to the 200 degrees than if it where in a pressure cooker. If you do do it this way spray the oven sterile. I have used a microwave with good sucess in the past for LC i usually did 7 min stop wait 2 repeat 3-4 times


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Re: Liquid medium, serilization in the oven? [Re: xburn]
    #5031998 -

Depending on whether your jar lids are metal or plastic and the size of your microwave, you might be able to do what this tek http://www.shroomery.org/index.php/par/24254 advises, and sterilize your jars and medium using the microwave, as quoted below.

"Karo sterilization:

Note: Clean the microwave out. Clean it as well as possible with bleach.

Place the jar into your microwave. Set the heat setting on high. Cook it for 1 minute. Wait 5 minutes and cook for another minute. CAUTION THE JAR WILL BE HOT! Leave it in the Microwave over night. This will keep it clean as it cools. "


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