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InvisibleNDK
member
Registered: 07/13/01
Posts: 186
Factors in achieving rhizomorhic growth on agar
    #422853 - 10/12/01 04:17 AM (22 years, 4 months ago)

Anyone know what factors influence rhizomorhic growth from tissue culture on agar.

I notice that on many clone attempts that the reculting mycelial growth is intermediate - slightly ropey but very dense.

Is it a random thing that requires multiple samples or does the agar recipe make a difference?

Is there anyway to get that intermediate mycelium to turn more rhizomorphic?

Any pointers much appreciated


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Invisibleoscill8
*bondage fairy*
Registered: 02/26/01
Posts: 1,215
Re: Factors in achieving rhizomorhic growth on agar *DELETED* [Re: NDK]
    #423129 - 10/12/01 11:31 AM (22 years, 4 months ago)

Post deleted by oscill8


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? oscill8 2001, 2002
"Any data submitted to Shroomery.org becomes our property"

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Offlinemarx2k
journeyman

Registered: 05/12/01
Posts: 76
Loc: Midwest
Last seen: 2 years, 1 month
Re: Factors in achieving rhizomorhic growth on agar [Re: oscill8]
    #423154 - 10/12/01 11:50 AM (22 years, 4 months ago)

My friend, she used malt extract agar (mixed from actual agar agar, malt extract, yeast and like 1ml of H2O2). There was rhizo growth from the first tissue samples she introduced, but the multispore was, of course, cottony, rhizo, etc...everything in there. She just transfers the rhizo part to another dish and voila! Its all rhizo from there! Woohoo!!

"If I don't wash for 30 days, will mushrooms grow in my armpits? I think it's much easier that way than knocking up some jars" - Steven Shteyn


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Alright alright

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