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 TrippinRhino
addict
Registered: 03/23/01
Posts: 342
Loc: TX
Last seen: 17 years, 10 months
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 Pan Cyans giving me hell. 
#417591 - 10/08/01 02:57 AM (22 years, 1 month ago) |
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Hopefully, some of y'all can help. I aquired a Pan Cyans print a little while back. By the time it got through the mail there was nothing really left of it. I scraped and scraped and got a little bit of dust from it along with some paper lint...lol. I shot up 6, 4oz jars of Rye/BRF/Verm. After a good while, only 1 grew. I took that jar and spawned 3 half pints of homemade dung compost which I planed to mix with wheat straw when colonized. They have colinized about 80% and have slowed down bigtime. Meanwhile, after the whole 1 jar episode, I bought another Pan print, again there wasn't much there when I recieved it. I made some 4oz jars of birdseed with a little BRF. Shot up Aussies and Pans at the same time. It has been 8 days and no signs of any life in my Pan jars. Meanwhile the Aussies are almost done. I assume that nothing is wrong with the substrate since Ps. cub is devouring it but WHAT THE HELL? Do Pans take a long time to germinate. I feel as though I have no prob with Ps. cub. Anyone have a suggestion for me?
On the formentioned homemade dung compost. It isn't all that. I am working on a new little batch that should be much better. My main concern is the lack of growth in the new jars. Should i be looking to potential problems with the print and not problems with my procedure?
For Premium prints and cultivation info check out www.thehawkseye.com
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All rhinos are endangered species...save the rhinos
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Anonymous
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 Re: Pan Cyans giving me hell. [Re: TrippinRhino]
#417876 - 10/08/01 09:17 AM (22 years, 1 month ago) |
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I never use syringes, so I don't know about your technique.
But I found the Cop. cyans to be very fast compared to P. cubensis. I germinate spores on agar and transfer to Manure:ryeseed:perlite in quart jars. These run through in a max of 14 days, but usually in under 10 days. Once laid out in tray and cased with a very thin layer of Peat:perlite, the pin in about 7 days, and mature by the 13 day max. Everything is fast. I also found it almost impossible to get a bad Mating from the spores, every wedge I transfer results in a fruiting strain. Overall, I would say they are Easy, and that is not an overstatement. All of the prints I've made from this manure based substrate are dark, and I always use paper for prints that I use myself. I would think that it was probably the condition of the print and the making of syringe, that was the problem, but then you said your jars stalled out. That sounds like a contaminant problem or a substrate problem. The Cop. cyans, are weak looking, but they are aggressive colonizers. If contamination is present, they will fall victim to it, but if none is present, there should be no reason for stalling, other than substrate problems, i.e moisture content, etc...
Try and avoid so many steps, transfers. I use mycelium from a petri dish to innoculate the manure based substrate directly, then lay out and case in trays. Since you are starting with a syringe, try innoculating rye grain, and use this to innoculate manure based substrate. Do you have a pressure cooker?
Too many steps opens up the possiblities of something going wrong. Syringe to rye grain to manure based substrate.
I would avoid straw, it just increases the chance of failure.
Maybe someone with a little more syringe experience can help you more.
Peace Teonan
You probably can innoculate the manure based substrate directly with a syringe, it shouldn't be this much of a problem.
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TrippinRhino
addict
Registered: 03/23/01
Posts: 342
Loc: TX
Last seen: 17 years, 10 months
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Re: Pan Cyans giving me hell. [Re: ]
#418310 - 10/08/01 01:53 PM (22 years, 1 month ago) |
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I don't need to use a syringe. I have agar and dishes. I have only dabbled in single strain isolation on them. I really should have taken this route first since there was almost nothing left of the print. Instead, I opted to shoot up some little jars. I felt this would be more sterile for me. I fucked up! I will have to get yet another print and start over. I may try to remove the un-colonized portion of the dung jars I have and use them to innoculate some pastuerized dung. I did not realize that you could go straight from agar to the dungbased substrate. I thought about the agar to rye to dung possibility but figure the shoot up jars had a little better chance than the shakables in the event of contams. I really don't have much trouble with contams come to think of it. I have had a few little spots on some pitre dishes but they were easily cut out. I WILL TRY AGAIN!
Will the Cop. Cyans germinate on the same agar as Ps. Cub?
Do you soak the spores first?
Thanks for your help Teonan!
For Premium prints and cultivation info check out www.thehawkseye.com
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All rhinos are endangered species...save the rhinos
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Anonymous
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Re: Pan Cyans giving me hell. [Re: TrippinRhino]
#418797 - 10/08/01 08:45 PM (22 years, 1 month ago) |
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I use MEA for all species!!!! Supplement with yeast and peptone, optional.
The cop. cyans look more cottony then the cubensis, don't look for Rhizomorphs, just transfer clean sectors. Like I said, they all have fruited well for me.
Good Luck
I should have some more prints in about a week, if you want to wait, I can send you a few.
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TrippinRhino
addict
Registered: 03/23/01
Posts: 342
Loc: TX
Last seen: 17 years, 10 months
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Re: Pan Cyans giving me hell. [Re: ]
#418944 - 10/08/01 11:04 PM (22 years, 1 month ago) |
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I would be greatful! I have been eager to try them. I hear the expirience is sublime.
I have malt yeast agar.
For Premium prints and cultivation info check out www.thehawkseye.com
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All rhinos are endangered species...save the rhinos
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Anonymous
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Re: Pan Cyans giving me hell. [Re: TrippinRhino]
#419588 - 10/09/01 12:49 PM (22 years, 1 month ago) |
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I will let you know when they are ready!!!
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