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OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,421
Loc: Greece
Last seen: 2 years, 7 months
agar quick question!
    #3804485 - 02/19/05 07:49 PM (19 years, 1 month ago)

hey gusys, i just made some PDA.
i PCed it and it became jelly and i forgot to add the peroxide when i should.
can i PC it again to make it liquid again and then add the peroxide?
thanks?

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OfflineAnnoA
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Registered: 06/17/99
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Re: agar quick question! [Re: Hamurabi]
    #3805500 - 02/20/05 12:31 AM (19 years, 1 month ago)

Yrs, but pressure cook it just as long to get liquid again.

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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: agar quick question! [Re: Anno]
    #3806208 - 02/20/05 09:29 AM (19 years, 1 month ago)

But, how long is that?


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OfflineAnnoA
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Re: agar quick question! [Re: Semilanceata]
    #3806296 - 02/20/05 10:29 AM (19 years, 1 month ago)

Depends on how much agar there is in the flask, how cold the agar is to begin with and how fast the pressure cooker gets hot.

IME it takes 10 - 15 minutes after the pressure has been reached for 500 ml agar to melt again. You time might vary.

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OfflineMycelium5150
Check theDate!!!!
Registered: 04/05/99
Posts: 541
Last seen: 19 years, 30 days
Re: agar quick question! [Re: Anno]
    #3806307 - 02/20/05 10:35 AM (19 years, 1 month ago)

doesnt the peroxcide evap rather quickly? What is the purpose of peroxcide in agar? To help sterlize the agar or to kill contams that may enter while doing transfers


--------------------
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I am here to Myth Bust

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OfflineAnnoA
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Re: agar quick question! [Re: Mycelium5150]
    #3806324 - 02/20/05 10:39 AM (19 years, 1 month ago)

The purpose it to guard the agar against spores of contaminant spores and bacteria.

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OfflineMycelium5150
Check theDate!!!!
Registered: 04/05/99
Posts: 541
Last seen: 19 years, 30 days
Re: agar quick question! [Re: Anno]
    #3806341 - 02/20/05 10:47 AM (19 years, 1 month ago)

gotcha, Does it help, is it better then fungi perfectis antibiotic agar?


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Mad skills, you know this!!!

I am here to Myth Bust

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