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agar quick question!
    #3804485 -

hey gusys, i just made some PDA.
i PCed it and it became jelly and i forgot to add the peroxide when i should.
can i PC it again to make it liquid again and then add the peroxide?
thanks?

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Re: agar quick question! [Re: Hamurabi]
    #3805500 -

Yrs, but pressure cook it just as long to get liquid again.

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Re: agar quick question! [Re: Anno]
    #3806208 -

But, how long is that?


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Sr_Setahongo

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Re: agar quick question! [Re: Semilanceata]
    #3806296 -

Depends on how much agar there is in the flask, how cold the agar is to begin with and how fast the pressure cooker gets hot.

IME it takes 10 - 15 minutes after the pressure has been reached for 500 ml agar to melt again. You time might vary.

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Re: agar quick question! [Re: Anno]
    #3806307 -

doesnt the peroxcide evap rather quickly? What is the purpose of peroxcide in agar? To help sterlize the agar or to kill contams that may enter while doing transfers


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Mad skills, you know this!!!

I am here to Myth Bust

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Re: agar quick question! [Re: Mycelium5150]
    #3806324 -

The purpose it to guard the agar against spores of contaminant spores and bacteria.

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Re: agar quick question! [Re: Anno]
    #3806341 -

gotcha, Does it help, is it better then fungi perfectis antibiotic agar?


--------------------
Mad skills, you know this!!!

I am here to Myth Bust

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