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Amazon Shop: Agar, Potato Dextrose, Pressure Cooker

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OfflineHamurabi
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Registered: 03/31/02
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agar quick question!
    #3804485 - 02/19/05 09:49 PM (13 years, 4 months ago)

hey gusys, i just made some PDA.
i PCed it and it became jelly and i forgot to add the peroxide when i should.
can i PC it again to make it liquid again and then add the peroxide?
thanks?


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OfflineAnnoA
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Re: agar quick question! [Re: Hamurabi]
    #3805500 - 02/20/05 02:31 AM (13 years, 4 months ago)

Yrs, but pressure cook it just as long to get liquid again.


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InvisibleSemilanceata
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Registered: 05/26/03
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Re: agar quick question! [Re: Anno]
    #3806208 - 02/20/05 11:29 AM (13 years, 4 months ago)

But, how long is that?


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OfflineAnnoA
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Re: agar quick question! [Re: Semilanceata]
    #3806296 - 02/20/05 12:29 PM (13 years, 4 months ago)

Depends on how much agar there is in the flask, how cold the agar is to begin with and how fast the pressure cooker gets hot.

IME it takes 10 - 15 minutes after the pressure has been reached for 500 ml agar to melt again. You time might vary.


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OfflineMycelium5150
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Registered: 04/06/99
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Re: agar quick question! [Re: Anno]
    #3806307 - 02/20/05 12:35 PM (13 years, 4 months ago)

doesnt the peroxcide evap rather quickly? What is the purpose of peroxcide in agar? To help sterlize the agar or to kill contams that may enter while doing transfers


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OfflineAnnoA
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Re: agar quick question! [Re: Mycelium5150]
    #3806324 - 02/20/05 12:39 PM (13 years, 4 months ago)

The purpose it to guard the agar against spores of contaminant spores and bacteria.


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OfflineMycelium5150
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Re: agar quick question! [Re: Anno]
    #3806341 - 02/20/05 12:47 PM (13 years, 4 months ago)

gotcha, Does it help, is it better then fungi perfectis antibiotic agar?


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Amazon Shop: Agar, Potato Dextrose, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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