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 ragadinks
MrBeatle
Registered: 10/20/03
Posts: 1,298
Last seen: 6 months, 6 days
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 Pics of King oyster on peroxide substrate 
#3542201 - 12/25/04 01:58 PM (19 years, 3 months ago) |
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Someone I know has send me these two pics of Pleurotus eryngii he has grown with the peroxide method:
 
Substrate was a mixture of 1/3 wood fuel pellets, 1/3 straw and 1/3 wheat grain supplemented with bran.
Yield was 1 kilogram good quality mushrooms from 3 kg of moist substrate.
Not bad, what do you think ?
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Cryogenicz
what?
Registered: 07/01/04
Posts: 2,421
Loc: Oregon
Last seen: 4 years, 10 months
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Re: Pics of King oyster on peroxide substrate [Re: ragadinks]
#3543100 - 12/25/04 09:55 PM (19 years, 3 months ago) |
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Thats awesome!
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 Alkaloids
3,4,5-trimethoxyphenethylamine
Registered: 11/15/98
Posts: 743
Loc: pubis mons
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Re: Pics of King oyster on peroxide substrate [Re: ragadinks]
#3544612 - 12/26/04 04:10 PM (19 years, 3 months ago) |
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Very nice. Those yields are very good also.
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YidakiMan
Stranger
Registered: 09/28/02
Posts: 2,023
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Re: Pics of King oyster on peroxide substrate [Re: Alkaloids]
#3544743 - 12/26/04 05:03 PM (19 years, 3 months ago) |
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That is very cool. It really shows that chemical processing of the substrate is just as viable as heat treatment, if not moreso.
I have been thinking of a P. eryngii substrate that consisted of; crushed corn cob, sawdust, and brassinosteroid supplement or concentrate supplement (ie bran or a meal).
After researching brassinosteroids, bee pollen is just about the best source. It has about the same protein as cottonseed meal but costs about 20x as much. So I was planning research on the effectiveness of the supplement.
I was planning the substrate to be lime processed. Lime is REAL cheap and readily availabe. I've had high success with straw and this method.
Lastly to improve the success rate I would inoculate at the highest rate possible. 1:5 dry spawn to dry substrate ratio. This high rate of spawning will also help boost yields.
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ragadinks
MrBeatle
Registered: 10/20/03
Posts: 1,298
Last seen: 6 months, 6 days
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Re: Pics of King oyster on peroxide substrate [Re: YidakiMan]
#3544996 - 12/26/04 06:19 PM (19 years, 3 months ago) |
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> I was planning the substrate to be lime processed.
> Lime is REAL cheap and readily availabe. I've had high success with straw and this method.
Never heard of that method. How do you treat the straw with the lime ?
And what kind of mushrooms did you grow on the lime treated substrate ?
> After researching brassinosteroids, bee pollen is just about the best source.
> It has about the same protein as cottonseed meal but costs about 20x as much.
> So I was planning research on the effectiveness of the supplement.
You mean that bee pollen cost about 20x as much as cottonseed meal ?
I always would use the cheapest available substrate for supplementing.
In my experience the easiest way to supplement substrate is by adding enough grain spawn or something similar to it.
The advantage is that the supplement is already fully colonized and even when you add it to pasteurized (not fully sterile) substrate you wont get contamination's easily.
If you add "hot" supplement that has not been already colonized to pasteurized substrate you will get contamination's more easily cause also molds are eager to colonize that.
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YidakiMan
Stranger
Registered: 09/28/02
Posts: 2,023
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Re: Pics of King oyster on peroxide substrate [Re: ragadinks]
#3545469 - 12/26/04 07:50 PM (19 years, 3 months ago) |
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The lime bath method is in GGMM (Stamets). 2-4 lbs of agricultural lime is added to 50 gal of water. The pH is around 11-12. The substrate is soaked for 16-24 hours. The substrate pH when removed is around 8.5-9. In GGMM, Stamets says the ideal pH at spawning for P. eryngii is 8.5.
I grew P. ostreatus on wheat straw processed in that method.
The process doesnt sterilize seed, so the wheat straw germinated while it was being colonized.
In my research bee pollen costs $8 a pound. Cottonseed meal costs less than 30 cents a pound. I was hard convinced that it was worth the difference. But, according to some, brassinosteroids greatly speed colonization. And I thought with chemical processing, speed of colonization is even more important. I agree with you on supplementing. In most cases, the best supplementation is more spawn. So for our King Oyster substratum, we're now down to crushed corncob and sawdust and spawn.
With the absence of the alfalfa straw, I think the that the substrate would become to dense and anaerobic. So maybe simply chopped wheat or oat straw should be added for ease of mycelial penetration but not out of any nutritional need.
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ragadinks
MrBeatle
Registered: 10/20/03
Posts: 1,298
Last seen: 6 months, 6 days
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Re: Pics of King oyster on peroxide substrate [Re: YidakiMan]
#3546812 - 12/27/04 03:25 AM (19 years, 3 months ago) |
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> The lime bath method is in GGMM (Stamets). 2-4 lbs of agricultural lime is added to 50 gal of water.
Ah, now I remember ... Think there was an article in the first mushroom handbook at www.mushworld.com.
> So for our King Oyster substratum, we're now down to crushed corncob and sawdust and spawn.
Think chrushed corncobs must be easy to get and cheap ?
What do you use as spawn ? And how are the yields ?
> With the absence of the alfalfa straw, I think the that the substrate would become to dense and anaerobic.
> So maybe simply chopped wheat or oat straw should be added for ease of mycelial penetration but not out of any nutritional need.
Yeah, I also add some straw to the almost any substrate I use cause it makes the substrate less dense.
Especially sawdust or coffee are getting colonized faster and are therefore less prone to contams.
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Corporal Kielbasa
Registered: 05/29/04
Posts: 17,235
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Re: Pics of King oyster on peroxide substrate [Re: ragadinks]
#3568162 - 01/01/05 12:40 PM (19 years, 3 months ago) |
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Thanks for the heads up  
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jmeuser
Eco-logic
Registered: 11/12/04
Posts: 32
Loc: Golden, Colorado
Last seen: 18 years, 9 months
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> The pH is around 11-12. The substrate is soaked for 16-24 hours. The substrate pH when removed is around 8.5-9.
In the lime method for chemical pasteurization, what neutralizes the pH while the substrate is being soaked?
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-Jon
Have:
Pleurotus pulmonarius (Pheonix Oyster)
Letinula edodes (Shitake)
Ganoderma lucidum (Reishi)
Want for trade:
Other edibles . . .
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debianlinux
Myconerd - DBK
Registered: 12/09/02
Posts: 8,334
Loc: Over There
Last seen: 9 months, 18 days
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Re: Pics of King oyster on peroxide substrate [Re: jmeuser]
#3683069 - 01/26/05 12:46 PM (19 years, 2 months ago) |
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the straw itself (or more accurately, the pH-affecting constituents of the straw). assuming that the soaked straw had absorbed all of the soaking water and all of the lime as well then the substrate pH would be much higher. While the substrate pH had been raised a couple points I'd bet the leftover soak water still had a very high pH.
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ShroomOmatic
Ethno Apprentice
Registered: 10/14/04
Posts: 2,373
Loc: Sailing the Seas of Chees...
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Re: Pics of King oyster on peroxide substrate [Re: Alkaloids]
#3683172 - 01/26/05 01:12 PM (19 years, 2 months ago) |
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Those look great 
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anthiawe
friendly stranger
Registered: 05/18/16
Posts: 652
Last seen: 2 years, 9 months
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Re: Pics of King oyster on peroxide substrate [Re: ShroomOmatic]
#23470192 - 07/23/16 06:28 PM (7 years, 8 months ago) |
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very nice!
is peroxide an economically viable method?
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TEK compendium
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Paresthesia
Stranger
Registered: 07/02/08
Posts: 1,090
Loc: Texas
Last seen: 5 years, 2 months
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Re: Pics of King oyster on peroxide substrate [Re: anthiawe]
#23471569 - 07/24/16 07:43 AM (7 years, 8 months ago) |
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No, not really. If you don't have access to a laminar flow hood it might be a good place to start to build other cultivation skills. You can do some basic tissue cloning with peroxide agar but no spore work.
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"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."
- T. S. Eliot
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