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Offlinedrzira
mexican grower
Registered: 07/28/03
Posts: 44
Loc: earth year 2004
Last seen: 10 years, 1 month
king oyster mycelium grows slow
    #1757075 - 07/28/03 08:11 PM (13 years, 4 months ago)

i put a cutting of the mushroom on a potato starch agar. at the beginning was going really fast. the mycelium grew furry on the top of the mushroom but just a little on the agar. now it seems it has stopped. can someone explain if there is something wrong?

p.s. i have added one tiny drop of peroxyde to the agar. also the local temp. is quite high about 29 celsius 84f.


Edited by drzira (07/28/03 08:15 PM)


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Offlinezeronio
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Re: king oyster mycelium grows slow [Re: drzira]
    #1758210 - 07/29/03 02:08 AM (13 years, 4 months ago)

When you're cloning you have to transfer mycellium as soon as you see it growin to isolate it from contamns. Cut some healthy pieces and transfer it to new plates.


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Offlinedrzira
mexican grower
Registered: 07/28/03
Posts: 44
Loc: earth year 2004
Last seen: 10 years, 1 month
Re: king oyster mycelium grows slow [Re: zeronio]
    #1759096 - 07/29/03 10:09 AM (13 years, 4 months ago)

but there is not contamination! is the temperature slowing or killing the process?


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Invisiblepsyconaut
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Registered: 05/22/02
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Re: king oyster mycelium grows slow [Re: drzira]
    #1759131 - 07/29/03 10:30 AM (13 years, 4 months ago)

What makes you think it's mycelium? Never assume that if it grows and it's not a funky colour, that it's mycelium...

-psy


--------------------
It may look like a button mushroom right now, but wait until you see how it grows!


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Offlinezeronio
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Re: king oyster mycelium grows slow [Re: drzira]
    #1761651 - 07/30/03 02:54 AM (13 years, 4 months ago)

Contaminants may not be visible at this point - even the white mycellium you see could not be from the desired speces. You really have to do several transfers before you can be sure that you got a clean culture.
The problem could be also in your agar formula (level of peroxide). Just another reason to do more transfers.
And the temperature is not too high IMO.


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Offlinedrzira
mexican grower
Registered: 07/28/03
Posts: 44
Loc: earth year 2004
Last seen: 10 years, 1 month
Re: king oyster mycelium grows slow [Re: zeronio]
    #1763758 - 07/30/03 08:26 PM (13 years, 4 months ago)

it has now stopped growing and totally receding. i will start again and i am going to try to transfer.


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