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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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InvisibleMr. Fryd
member

Registered: 10/31/00
Posts: 77
A. blazei mycelium question
    #344757 - 06/19/01 10:11 PM (23 years, 7 months ago)

I recently recieved an a blazei culture that promptly went into the incubator, 81 degrees f.

It was been in the incubator for a few weeks now and while the culture looks healthy, dimesized, there is no new growth.

This culture is on PDA, with no visible contams. Parafilmed, beautiful to look at really.

Am I keeping it too warm?? I have seen temps listed for all stages of growth except on agar in the GGMM, new updated addition.

Please help, im very anxious to kick this bugger off.



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Better living thru Mycology.....

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OfflineSuntzu
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Registered: 10/14/99
Posts: 1,396
Last seen: 27 days, 10 hours
Re: A. blazei mycelium question [Re: Mr. Fryd]
    #345132 - 06/20/01 11:19 AM (23 years, 7 months ago)

Those temps should be fine, all the isolations I've done were at RT.
I noticed that it tends to grow slowly laterally, but likes to grow vertically up against the petri top. There will be lateral growth eventually, assuming everything is viable.
Of course, if it was in a freezing cargo hold before reaching you, who knows :)

I only eat veils

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InvisibleMr. Fryd
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Registered: 10/31/00
Posts: 77
Re: A. blazei mycelium question [Re: Suntzu]
    #345324 - 06/20/01 03:55 PM (23 years, 7 months ago)

It came from you buddy.

Cheers and thanks for the info friend.

MM



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OfflineAzure
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Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 22 years, 8 months
Re: A. blazei mycelium question [Re: Mr. Fryd]
    #346832 - 06/22/01 05:32 PM (23 years, 7 months ago)

Well, although I may not have the answer to your question, I have a similar problem. When I transferred agar pieces into rye berry jars, the mycelium grew prolific and somewhat rhizomorphic. It was growing about as fast as cubes. As soon as 1/4 of the jar was colonized, I shook the jars to evenly distribute the colonized kernels and speed up colonization. I didn't shake one as a control. Every single one that was shaken didn't really recover. The one that wasn't shaken did just fine, and is still growing with vigor.
This mushroom may not like stress. I opened some of the jars two weeks after shaking and there was no bacterial smell. The colonized kernels were fuzzy, but completely stopped growing.


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InvisibleMr. Fryd
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Registered: 10/31/00
Posts: 77
Re: A. blazei mycelium question [Re: Azure]
    #346952 - 06/22/01 09:01 PM (23 years, 7 months ago)

So how would you suggest doing an agar to grain transfer??

I like to dice my colonized agar into tiny pieces and put it into the grain then shake it really good.

Ussually I get 40 - 50% colonization before I remove the foil and shake.

Should I not shake and just let it run through the grain??



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OfflineAzure
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Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 22 years, 8 months
Re: A. blazei mycelium question [Re: Mr. Fryd]
    #347107 - 06/23/01 12:35 AM (23 years, 7 months ago)

From what I'm observing with my cultures, yes, you should not shake the jars when you put the agar wedge into the grain. Also, you might want to take a piece of your mycelium now and put it into grain. That might get the mycelium running again. You can then use that jar as a grain spawn master. It might just be the strain I'm dealing with that's very sensitive to stress. keep me posted on how your strain does on grain. Try shaking a small jar of grain and see what happens.


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InvisibleMr. Fryd
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Registered: 10/31/00
Posts: 77
Re: A. blazei mycelium question [Re: Azure]
    #347580 - 06/23/01 07:17 PM (23 years, 7 months ago)

Another quick question about A blazei.

It the mycelium peroxide tolerant?? I plan on doing some agar to agar transfers soon onto MEA, peroxidated.

I generally use only peroxidated agar except to germ spores. I have no flow hood.



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OfflineAzure
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Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 22 years, 8 months
Re: A. blazei mycelium question [Re: Mr. Fryd]
    #348781 - 06/25/01 05:06 PM (23 years, 7 months ago)

I don't have a flow hood either, and I don't use peroxide in my cultures. When I mixed the colonized woodchips I got from FP with compost, I used hydrogen peroxide with no ill effect. However, I don't know how A. blazei mycelium responds to H2O2 when there is little mass...it seems to be very sensitive until it colonizes a lot of material.


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