Home | Community | Message Board


The Spore Depot
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood, Malt Extract, Petri Dish, Potato Dextrose, Rye Grain

Jump to first unread post. Pages: 1
InvisibleMr. Fryd
member

Registered: 11/01/00
Posts: 77
A. blazei mycelium question
    #344757 - 06/20/01 12:11 AM (15 years, 5 months ago)

I recently recieved an a blazei culture that promptly went into the incubator, 81 degrees f.

It was been in the incubator for a few weeks now and while the culture looks healthy, dimesized, there is no new growth.

This culture is on PDA, with no visible contams. Parafilmed, beautiful to look at really.

Am I keeping it too warm?? I have seen temps listed for all stages of growth except on agar in the GGMM, new updated addition.

Please help, im very anxious to kick this bugger off.



--------------------
Better living thru Mycology.....


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 29 days, 2 hours
Re: A. blazei mycelium question [Re: Mr. Fryd]
    #345132 - 06/20/01 01:19 PM (15 years, 5 months ago)

Those temps should be fine, all the isolations I've done were at RT.
I noticed that it tends to grow slowly laterally, but likes to grow vertically up against the petri top. There will be lateral growth eventually, assuming everything is viable.
Of course, if it was in a freezing cargo hold before reaching you, who knows :)

I only eat veils


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMr. Fryd
member

Registered: 11/01/00
Posts: 77
Re: A. blazei mycelium question [Re: Suntzu]
    #345324 - 06/20/01 05:55 PM (15 years, 5 months ago)

It came from you buddy.

Cheers and thanks for the info friend.

MM



--------------------
Better living thru Mycology.....


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 14 years, 7 months
Re: A. blazei mycelium question [Re: Mr. Fryd]
    #346832 - 06/22/01 07:32 PM (15 years, 5 months ago)

Well, although I may not have the answer to your question, I have a similar problem. When I transferred agar pieces into rye berry jars, the mycelium grew prolific and somewhat rhizomorphic. It was growing about as fast as cubes. As soon as 1/4 of the jar was colonized, I shook the jars to evenly distribute the colonized kernels and speed up colonization. I didn't shake one as a control. Every single one that was shaken didn't really recover. The one that wasn't shaken did just fine, and is still growing with vigor.
This mushroom may not like stress. I opened some of the jars two weeks after shaking and there was no bacterial smell. The colonized kernels were fuzzy, but completely stopped growing.



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMr. Fryd
member

Registered: 11/01/00
Posts: 77
Re: A. blazei mycelium question [Re: Azure]
    #346952 - 06/22/01 11:01 PM (15 years, 5 months ago)

So how would you suggest doing an agar to grain transfer??

I like to dice my colonized agar into tiny pieces and put it into the grain then shake it really good.

Ussually I get 40 - 50% colonization before I remove the foil and shake.

Should I not shake and just let it run through the grain??



--------------------
Better living thru Mycology.....


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 14 years, 7 months
Re: A. blazei mycelium question [Re: Mr. Fryd]
    #347107 - 06/23/01 02:35 AM (15 years, 5 months ago)

From what I'm observing with my cultures, yes, you should not shake the jars when you put the agar wedge into the grain. Also, you might want to take a piece of your mycelium now and put it into grain. That might get the mycelium running again. You can then use that jar as a grain spawn master. It might just be the strain I'm dealing with that's very sensitive to stress. keep me posted on how your strain does on grain. Try shaking a small jar of grain and see what happens.



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMr. Fryd
member

Registered: 11/01/00
Posts: 77
Re: A. blazei mycelium question [Re: Azure]
    #347580 - 06/23/01 09:17 PM (15 years, 5 months ago)

Another quick question about A blazei.

It the mycelium peroxide tolerant?? I plan on doing some agar to agar transfers soon onto MEA, peroxidated.

I generally use only peroxidated agar except to germ spores. I have no flow hood.



--------------------
Better living thru Mycology.....


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAzure
old hand
Registered: 01/01/99
Posts: 469
Loc: California, USA
Last seen: 14 years, 7 months
Re: A. blazei mycelium question [Re: Mr. Fryd]
    #348781 - 06/25/01 07:06 PM (15 years, 5 months ago)

I don't have a flow hood either, and I don't use peroxide in my cultures. When I mixed the colonized woodchips I got from FP with compost, I used hydrogen peroxide with no ill effect. However, I don't know how A. blazei mycelium responds to H2O2 when there is little mass...it seems to be very sensitive until it colonizes a lot of material.



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood, Malt Extract, Petri Dish, Potato Dextrose, Rye Grain

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Similar ThreadsPosterViewsRepliesLast post
* A. brasiliensis mycelium question armedia 404 0 07/17/04 12:05 PM
by armedia
* Agaricus blazei murrill baycafe 681 2 10/08/07 12:25 PM
by Jeremy_Davis
* Shiitake mycelium (pic) sakura 1,840 7 04/05/04 09:11 AM
by Mycena
* Agaricus blazei Murill brainnoise 2,271 13 12/22/06 10:29 PM
by LentinulaEdodes
* Some Answers to Your Questions Regarding Making Money Growing Mushrooms
( 1 2 all )
Jeremy_Davis 25,378 32 11/13/12 01:37 AM
by bitepourrie
* Creating grain spawn with the peroxide method ragadinks 4,524 16 03/22/05 04:30 AM
by jmeuser
* Need to Identify this mycelium vonswarrior 1,866 14 08/13/08 05:31 PM
by vonswarrior
* Portabella Questions BigBlackNinja 2,214 11 08/04/04 03:53 AM
by NextKind

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, EvilMushroom666, cronicr, Pastywhyte
1,805 topic views. 3 members, 17 guests and 5 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Phytoextractum
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.038 seconds spending 0.004 seconds on 14 queries.