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OfflineCloneufc
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A. blazei mycelium question?
    #12800444 - 06/25/10 12:10 AM (13 years, 9 months ago)

Are you not suppose to refrigerate A. blazei mycelium? I put a couple petri dishes in the fridge and the ones I put in there don't do anything when I transfer them to grain. I know your not suppose to refrigerate P. Tuber but I do it without any problems. A Blazei seems to be the pickiest culture I have ever run across.

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InvisibleMonkeyKnifeFight
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Re: A. blazei mycelium question? [Re: Cloneufc]
    #12801891 - 06/25/10 10:46 AM (13 years, 9 months ago)

This thread indicates A. subrufescens/blazei cultures can't survive under 50 degrees (I'm gonna assume F).

http://www.shroomery.org/forums/showflat.php/Number/8747793#8747793

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OfflineCloneufc
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Re: A. blazei mycelium question? [Re: MonkeyKnifeFight]
    #12801917 - 06/25/10 10:51 AM (13 years, 9 months ago)

Ohh great I just fucked my culture up. I put the petri dishes out in the open. I hope they recover. Damn I'm pissed!

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OfflineMephistophelian
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Re: A. blazei mycelium question? [Re: Cloneufc]
    #12801943 - 06/25/10 10:55 AM (13 years, 9 months ago)

What's this nonsense all about?

Why the hell isn't this common knowledge...I'm going to check and see if I didn't just kill my culture too.

Goddamnit! :crankey:


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Offlinesolumvita
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Re: A. blazei mycelium question? [Re: Mephistophelian]
    #12802257 - 06/25/10 11:59 AM (13 years, 9 months ago)

Blaei is also sensitive with culture degradation, make a lot of petri's to ensure you keep your p value as low as possible.


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OfflineCloneufc
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Re: A. blazei mycelium question? [Re: solumvita]
    #12802429 - 06/25/10 12:38 PM (13 years, 9 months ago)

I was thinking about this the other day. I was thinking that in Brazil it doesn't snow. After three grain transfers and no growth I knew something was wrong. I will store it at room temps in distilled water in the future.

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Offlinecurenado
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Re: A. blazei mycelium question? [Re: Cloneufc]
    #12802886 - 06/25/10 02:01 PM (13 years, 9 months ago)

Thanks guys!
I made my spawn off of Meph's spores and haven't made or fridged a culture yet - glad to know and since I have some running if anyone loses thier shit I'll help back w/ prints.
Lots to know about these creatures even for vets!


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InvisibleMonkeyKnifeFight
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Re: A. blazei mycelium question? [Re: curenado]
    #12803462 - 06/25/10 03:47 PM (13 years, 9 months ago)

I can't find much mention of this temperature sensitivity on the interwebs.  But the mushroompeople site also mentions subrufescens needs to be stored above 50F.

mushroompeople - subrufescens

Though to make it more confusing mushroompeople has both blazei and subrufescens cultures on their list but wikipedia and other articles I found said that A. blazei was the same as A. subrufescens just an out of date name or something.  So that would imply the same growth parameters but mushroompeople's blazei page doens't mention the temperature thing.  Seems like if the fridge is gonna kill cultures that would be stuck there in bold since it seems to be a pretty common method to throw cultures in the fridge.

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OfflineMephistophelian
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Re: A. blazei mycelium question? [Re: MonkeyKnifeFight]
    #12803474 - 06/25/10 03:51 PM (13 years, 9 months ago)

I'll pull my culture out and test it in a few days...we'll know for sure soon enough, but thank you Monkey :thumbup:


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InvisibleCordyceps
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Re: A. blazei mycelium question? [Re: Mephistophelian]
    #12803538 - 06/25/10 04:00 PM (13 years, 9 months ago)

Ok good to know that this species also must be keep at room temperature. Very fine that a book like Growing Gourmets and Medicinal Mushrooms nothing tell about the storage temperature the same as Pleurotus tuber-regium what also be stored at room temperature.

I think it is a good idea to make a thread where to warn about species what must be kept at room temperature ! and ask to RR if he will put it sticky

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OfflineCloneufc
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Re: A. blazei mycelium question? [Re: Cordyceps]
    #12805912 - 06/26/10 01:27 AM (13 years, 9 months ago)

I'm guessing any species that grows where it never snows, should be kept in distilled water at room temperature. Cubensis surprises me in that it's tropical but can be kept in the fridge. I have been keeping a close eye on my cultures and so far no growth. I will wait another two days.

Edited by Cloneufc (06/26/10 01:31 AM)

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Offlinetroncotron
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Re: A. blazei mycelium question? [Re: Cloneufc]
    #12806445 - 06/26/10 05:23 AM (13 years, 9 months ago)

I keep the agaricus blazeei mycelia in liquid culture at 4ÂșC  and works well. Much better in liquid than in solidified media. About 30% of the plates I did from the P1 dish aborted, but otherwise they grew very aggressive in 3g/L of malt extract liquid media.

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InvisibleHacendado
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Re: A. blazei mycelium question? [Re: curenado]
    #12811449 - 06/27/10 10:16 AM (13 years, 8 months ago)

How did you prepare your spawn? I took hpoo, garden compost, coir, mixed it, and serilized it. 6 days and still no growth. I'm also using MS
Thank you!

Edited by Hacendado (06/27/10 10:26 AM)

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Offlinecurenado
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Re: A. blazei mycelium question? [Re: Hacendado]
    #12811485 - 06/27/10 10:33 AM (13 years, 8 months ago)

I just put some on popcorn - later I wondered if some additives might have been better because it took awhile to run but it ran nicely enough.
Spawned it to a tub of straw/hpoo and going to expand it to a mostly poo/compost/top soil patch with some straw as a fluffer.

But I was just guessing "first time blind"....


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InvisibleHacendado
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Re: A. blazei mycelium question? [Re: curenado]
    #12811808 - 06/27/10 11:46 AM (13 years, 8 months ago)

So it worked out for you?:cool:
I'm going to try to do it on rye and on rye+gypsum and see what would happen.
I have read that A. Blazeii spores wouldn't germinate from MS in seed but only in compost mix so I've made one. Now this encourages me to try with rye I would love if I could make spawn jars out of MS. It would be much easier:dancer: Is it a little bit too short to expect germination within 6 days?:oogle:

I was thinking of doing exactly the same thing with my patch:thumbup:

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Offlinecurenado
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Re: A. blazei mycelium question? [Re: Hacendado]
    #12812127 - 06/27/10 12:35 PM (13 years, 8 months ago)

It took so long I thought they had failed and then just noticed that they were going. I lost the first three to mold while one ran textbook and the other just behind it. They ran slow too. I lost another one to mold by the time I had two run quarts but yeah - made it with something.
HoudiniHar is the blazei guy I think...

I did mine from multispore that I got from Mephi which may be a descendent of houdinihar not sure about all that...


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Yours in the Natural State!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."

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