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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Waking up A.blazei spores
    #11554671 -

In late October, I put some A.blazei spores on MYPA agar. They did not germinate, so I scoured this board for some ideas on what to do and a few people suggested putting other growing culture on the same plate. So, I went to the store and got some nice looking button mushrooms (A. bisporus) and put some of their inner cap tissue on the same plate.

For a while, it looked like A. bisporus pieces died (they turned brown/black), but after a few weeks, they started putting out some nice cottony mycelium. This was a nice surprise.

But I was in for an even bigger surprise. A few weeks ago, another area of the plate started growing - a place where the A. bisporus didn't touch. Could it be A.blazei spores waking up?




I isolated a section of this new growth and put it on a new plate. Here it is, as of today:



Any thoughts/comments on this? Is it likely to be A.blazei? Or is it something else (a ninja piece of A. bisporus that jumped around the plate? a contaminant? something else?)


--------------------
I am looking for the following edible cultures:
  • Chicken of the woods (Laetiporus sulphureus)
  • Wood blewit (Lepista nuda)
  • Cauliflower (Sparassis crispa)

My trade list of available cultures is in my Journal.

Edited by gribi (11/30/09 12:14 PM)

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Re: Waking up A.blazei spores [Re: gribi]
    #11554703 -

Looks like success to me. Great job!! I am currently trying to revive my a. blazei culture.


--------------------
Follow the light
The Light is your guide.

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Re: Waking up A.blazei spores [Re: fungus_tao]
    #11558373 -

great success.
houdinihar

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Re: Waking up A.blazei spores [Re: gribi]
    #11560644 -

Congratulations, that's how you do it.

Carsten

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Re: Waking up A.blazei spores [Re: Mycelio]
    #11560878 -

I have some Leucoagaricus americanus and Agaricus campestris spores that I've been trying to germinate with no success.  I'll have to give this a try!


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"We shall not cease from exploration, and the end of all our exploring will be to arrive where we started and know the place for the first time."

- T. S. Eliot

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Re: Waking up A.blazei spores [Re: Paresthesia]
    #11564034 -

Good for you Gribi!

Paresthesia said:
I have some Leucoagaricus americanus and Agaricus campestris spores that I've been trying to germinate with no success.  I'll have to give this a try!


I believe you can try your Chlorophyllum rhacodes for the leucoagaricus americanus.  That would be the most similar.

Jef


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I am my own lab rat.


Tell me and I will forget. Show me and I will remember.  Involve me and I will learn.

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Re: Waking up A.blazei spores [Re: Jef]
    #11564086 -

gribi, did you swipe the spores on that part of the plate?
Did you put the A. Bisporus tissue on a section that was swiped by your A Blezei spores?

The tissued section seems to be overlapping the new growth a little and the new growth is a bit thinner leading me to think it's growth from spores....could be wrong though.
I will watch.

There's only 1 way to find out.... :mushroomgrow:  :mushroomgrow:  :mushroomgrow:


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Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech,

Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have


Primitive Skills                                     

Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms 
...I DO advocate learning about
Law Enforcement Against Prohibition

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Re: Waking up A.blazei spores [Re: RonPaulVerm]
    #11564254 -

RonPaulVerm said:
gribi, did you swipe the spores on that part of the plate?



I used a spore syringe (i.e. spores in sterile water) - putting a few droplets on the plate.

Quote:
Did you put the A. Bisporus tissue on a section that was swiped by your A Blezei spores?



I can't say for sure, because by the time I put A. Bisporus tissue on the plate, the droplets have dried out. I believe it was not in the same spots though (that would make for some pretty odd coincidence).

Quote:
The tissued section seems to be overlapping the new growth a little and the new growth is a bit thinner leading me to think it's growth from spores....could be wrong though.
I will watch.



It looks that way to me too. New growth seems to stop at the border of the existing mycelial mat of A. bisporus.

Quote:
There's only 1 way to find out.... :mushroomgrow:  :mushroomgrow:  :mushroomgrow:


Exactly. It will be a while, but I'll update this post with my progress. Also, I should be able to detect the presence (or absence) of the almond smell on spawn.


--------------------
I am looking for the following edible cultures:
  • Chicken of the woods (Laetiporus sulphureus)
  • Wood blewit (Lepista nuda)
  • Cauliflower (Sparassis crispa)

My trade list of available cultures is in my Journal.

Extras: Filter Print Post Top
Re: Waking up A.blazei spores [Re: gribi]
    #11597401 -

Quick update: it grew out nicely, so it's time to put it on grain and see what it really is.



--------------------
I am looking for the following edible cultures:
  • Chicken of the woods (Laetiporus sulphureus)
  • Wood blewit (Lepista nuda)
  • Cauliflower (Sparassis crispa)

My trade list of available cultures is in my Journal.

Extras: Filter Print Post Top
Re: Waking up A.blazei spores [Re: gribi]
    #11599802 -

That's some fine looking growth !

Good growing,

Jef


--------------------
I am my own lab rat.


Tell me and I will forget. Show me and I will remember.  Involve me and I will learn.

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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