Home | Community | Message Board


Kratom Eye
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Microscope

Jump to first unread post. Pages: 1
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
question about Panaeolus Cyanescens mycelium
    #3433261 - 12/01/04 04:55 PM (12 years, 5 months ago)

A Pan. Cyanescens print was obtained in a trade and plated onto MEA. The resulting colony looked very similar to other pictures posted here. This primary culture was then transferred to several other plates.

The question is: Has anyone observed Pan. Cyanescens creating rampant satellite colonies during transfers? Careful observation of plate one (may or may not be obvious with these photos) shows that some of the colonies have a distinctive appearance that is not found in the original transfer segment. In plate two
all the satellite colonies appear identical, at least macroscopically, to the main segment.
Here are the two examples:



Any information that might shed some light on this phenomena would be great. The satellite colonies that were different than the original culuture had small spiky protrusions and were not as aerial/cottony.


--------------------


Edited by Alkaloids (12/03/04 03:18 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: question about Panaeolus Cyanescens mycelium [Re: Alkaloids]
    #3434751 - 12/01/04 10:23 PM (12 years, 5 months ago)

As a microscope is not available the spores could not be identified prior to plating. After referencing several texts no information concerning fragility of the mycelial chains could be found.

status=stumped


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,122
Loc: my room
Last seen: 1 month, 11 hours
Re: question about Panaeolus Cyanescens mycelium [Re: Alkaloids]
    #3435678 - 12/02/04 02:10 AM (12 years, 5 months ago)

Those satellite colonies look like developing green mold.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,000
Loc: USA Mountain Northwest
Last seen: 1 month, 22 days
Trusted Cultivator
Re: question about Panaeolus Cyanescens mycelium [Re: Anno]
    #3436637 - 12/02/04 11:46 AM (12 years, 5 months ago)

Or bacteria. In fact, the main colony doesn't look too good either. The top one looks like mold.


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: question about Panaeolus Cyanescens mycelium [Re: Anno]
    #3438171 - 12/02/04 05:09 PM (12 years, 5 months ago)

  That had been a thought also, but green mold has been a problem before and these colonies have not changed color at all.  Even the parent colony (over 2 weeks old) that these were taken from has not shown any signs of being the green death.

  Normally green mold changes to its mature form in that span of time, which is one reason that it has been mostly ruled out.  Although the original culture might have been contaminated and burst during the agar transfer.

  Thanks for the feedback.  :ooo:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: question about Panaeolus Cyanescens mycelium [Re: RogerRabbit]
    #3438209 - 12/02/04 05:15 PM (12 years, 5 months ago)

Sadly the resolution on the camera is not the best, but when viewed with the naked eye they appear to be fungi of some type.  Whether perfect or imperfect is hard to tell at the moment.  If they are bacteria then they are bacteria with aerial hyphae.  Which don't exist, in literature at least. :smile: 

  As prior experience with this strain is lacking and only two pictures on agar plates were found it has been very difficult to cross-reference the macroscopic features.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 6 days, 2 hours
Re: question about Panaeolus Cyanescens mycelium [Re: Alkaloids]
    #3439391 - 12/02/04 09:18 PM (12 years, 5 months ago)

Streptomyces are bacteria with aerial 'hyphae'. . .they smell like musty basements. I've never seen them spread like that though.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: question about Panaeolus Cyanescens mycelium [Re: Suntzu]
    #3440851 - 12/03/04 03:35 AM (12 years, 5 months ago)

  The smell is another consideration.  There is no strong smell associated with the parent culture (the only one which has been smelled so far).  No color changes of any kind.  As a matter of fact all the satellite colonies are blindingly white. 

  Thanks for the information on strepomyces.  :ooo:

  The colonies will be observed for several more days.  One or two jars of grain spawn might be started to further catalog any changes in morphology.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: question about Panaeolus Cyanescens mycelium [Re: Alkaloids]
    #3441900 - 12/03/04 01:22 PM (12 years, 5 months ago)

What Strain was it?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: question about Panaeolus Cyanescens mycelium [Re: EonTan]
    #3442306 - 12/03/04 03:19 PM (12 years, 5 months ago)

Panaeolus Cyanescens


Thanks for playing. *lol*


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: question about Panaeolus Cyanescens mycelium [Re: Alkaloids]
    #3444157 - 12/04/04 01:03 AM (12 years, 5 months ago)

What Strain not what Species!!!

Thanks for palying :rolleyes:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: question about Panaeolus Cyanescens mycelium [Re: EonTan]
    #3449966 - 12/05/04 02:17 PM (12 years, 5 months ago)

ooops.  :shocked:
  An unknown strain from Florida.  No other information was provided.  It was obtained from a trade a while ago.

  There will probably be some attempts at purchasing a known strain from a reliable vendor if this doesn't work out.

  Still no signs of this being the Green Death.  No color changes on any of the colonies.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: question about Panaeolus Cyanescens mycelium [Re: Alkaloids]
    #3454296 - 12/06/04 01:24 PM (12 years, 5 months ago)

None of the four strains of Copelandia cyanescens in circulation that originated from Florida have any asexual lifecycle component that would account for your cultures. They all undergo cell fragmentation as asexual cycle, none have any asexual spores that could be released to form new colonies. One does form pseudosclerotia in culture and in casings, but they don't release any spores or anything like that.

It is a weird thing you got going there. I would have just thought Ariborne contamination during transfer, and tossed the plates.

Definetly move on to a new print, becasue NONE of Pan/Cope cyanescens in circulation, and that is all that is in circulation, do what your cultures are doing.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: question about Panaeolus Cyanescens mycelium [Re: EonTan]
    #3460002 - 12/07/04 04:02 PM (12 years, 5 months ago)

  Thank you very much.  That was very useful information.  I didn't think any Copelandia/Panaelous Cyanescens had that type of hyphal fragmentation or development, but without additional information I couldn't confirm the identity.

  One plate will be kept for identification purposes. (New lab microscope will be here soon).  The rest will get tossed.  Die strange contaminant die! 

Still perplexed.  :confused:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinesinfull7
Stranger
 User Gallery
Registered: 12/16/04
Posts: 11
Last seen: 12 years, 2 months
Re: question about Panaeolus Cyanescens mycelium [Re: Alkaloids]
    #3572631 - 01/02/05 08:51 PM (12 years, 4 months ago)

most molds will show up fast enough to where you know for sure in 2-5 days at the most as long as the temps are in the correct range . 30c . here is a picture behaving normally just in case .there doesn't seem to be a lot of info what does better the linear are cottony myc and what does better in cultures. personally i like the cottony . in that it seems to do better with being more prolific. I've used both and the latter does produce fruits as well ..


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Agar, Malt Extract, Microscope

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Re: question on liquid mycelium... Psilowarrior 792 1 12/22/99 11:54 AM
by Coma of Souls
* rye bag question using liquid mycelium bent 702 4 06/03/04 11:41 AM
by bent
* Liquid Mycelium - Could contams lurk and not be visible?
( 1 2 all )
bent 3,430 32 08/12/04 07:53 PM
by hyphae
* spawing and genotype question... sluglee 747 6 11/11/02 05:05 PM
by tchyted
* dikaryotic mycelium questions LochRaven 4,032 18 05/05/06 03:02 PM
by texas34
* Re: Mycelium H2O + H2O2? joee 2,220 6 11/30/99 06:07 PM
by mycofile
* A dead horse subject: Liquid Mycelium, a new approach?
( 1 2 all )
doctor_kandee 5,550 24 09/23/05 08:46 PM
by doctor_kandee
* Several Psilocybe cyanescens questions LordByron 6,040 16 01/04/08 11:28 PM
by LordByron

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit
2,513 topic views. 0 members, 3 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
The Spore Depot
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.04 seconds spending 0.007 seconds on 18 queries.