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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

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OfflinesHrOoMeRrR420
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inoculation problem
    #3427488 - 11/30/04 01:39 PM (13 years, 13 days ago)

Hi, I just inoculated a few days ago and when I stuck the needle into one of the jars the cotton ball fell through. I was just wondering if you guys think this is a problem? (for contams... or anything else)

Also what do you guys think about boiling WBS? I heard WBS has to be pressure cooked will I have just as much success as someone who boils BRF or am I going to run into some problems with contam?


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Offlinebuckeye
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Re: inoculation problem [Re: sHrOoMeRrR420]
    #3427500 - 11/30/04 01:42 PM (13 years, 13 days ago)

a friend tried to do boil grains, it didn't work out. You may be able to try multiple boilings, like 1.5 hours for 3 days, but ur better off sticking with pf cakes if u don't have a pc.


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Invisiblewoodzmun
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Registered: 07/14/04
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Re: inoculation problem [Re: sHrOoMeRrR420]
    #3427513 - 11/30/04 01:47 PM (13 years, 13 days ago)

Sorry dude but you mucked up. First your not supposed to use cotton, use polyfil or tyvek. Not to mention that hole in the lid now letting airborne cotams in where the cotton was. And boiling birdseek, no way, grains must be PC'ed. I have heard talk about fractual sterilization where you boil it for a hour a day, for days on end, but I would just get a PC or stick to BRF.


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Invisibletripndicular
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Re: inoculation problem [Re: sHrOoMeRrR420]
    #3427559 - 11/30/04 02:04 PM (13 years, 13 days ago)

PC is best for grain , you can if desperate boil in coverd pot for 2 hours , and I mean truly keep it boiling for 2 hours , risky though , high contam rate to be expected .

As far as your screw up poking a jar with needle , cover that hole ASAP with some 3M micropore tape .
In the future use the old verm barrier method or the 3M micropore tape to cover holes and inhibit cotams from getting into the picture .
Good Luck


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Offlinespliffmasta
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Re: inoculation problem [Re: tripndicular]
    #3427595 - 11/30/04 02:11 PM (13 years, 13 days ago)

No PC way:

Generate a liquid culture.
Then either:
Put that on BRF and spawn to bulk
or
Noc up bulk directly with liquid culture.


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OfflinesHrOoMeRrR420
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Re: inoculation problem [Re: spliffmasta]
    #3436860 - 12/02/04 12:47 PM (13 years, 11 days ago)

Well I covered the jars with a tinfoil lid so no Im not a complete dumbass that left a hole in the top of the jar just sucking in airborne contams.


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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