Home | Community | Message Board


Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker

Jump to first unread post. Pages: 1
OfflineParticleMan
enthusiast
Registered: 02/05/02
Posts: 240
Last seen: 15 years, 4 months
innoculation problem?
    #555708 - 02/18/02 11:02 PM (15 years, 7 months ago)

my friend is doing very well but he has a very small question for the wise, he seems to have a problem somewhere in the innoculation phase. He mixes up all his substrate (BRF) puts it in his jars (1/2 pint) and then throws it in the old pressure cooker for about 45 min (10-15 psi) then he lets them cool overnight. this all seems normal to this point, but this is what throws him. He will innoculate at 4 sites and the sopres will only germinate and grow at the average on 2-3 points per jar. He shakes his syringes (which are less than 2 months old from the label) pretty well after drawing in a small amount of air in order for him to be able to mix it all up. Should he consider spraying it all out into a sterile jar and then sucking it all back up a few times to break it up? He is baffled, and this little problem slows his colonization in his beautiful incubator very much. He would appreciate any help. He has not lost any to contams (since the PC) and they evetually all colonize but very slowly due to it having to sometimes grow from only one point.


--------------------
___________________________________________________________
"the weekend has landed all that exists now is clubs,drugs, pubs, and parties" - Human Traffic


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 2 months, 26 days
Re: innoculation problem? [Re: ParticleMan]
    #555716 - 02/18/02 11:10 PM (15 years, 7 months ago)

Do you sterilize the needle between each jar?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineParticleMan
enthusiast
Registered: 02/05/02
Posts: 240
Last seen: 15 years, 4 months
Re: innoculation problem? [Re: Anno]
    #555933 - 02/19/02 03:37 AM (15 years, 7 months ago)

i sterilize it with a lighter usually, but this last time i decided to just use an alch wipe between every 2 jars, still the same problem arose.


--------------------
___________________________________________________________
"the weekend has landed all that exists now is clubs,drugs, pubs, and parties" - Human Traffic


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 2 months, 26 days
Re: inoculation problem? [Re: ParticleMan]
    #555950 - 02/19/02 04:10 AM (15 years, 7 months ago)

Hmm...



Perhaps it?s because you?re not inoculating the same amount of the spore suspension each hole? But at least some growth should emerge.

To be honest, I?m a bit helpless here.


Edited by Anno (06/05/03 06:25 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* inoculation problems with LC? houdinihar 301 0 03/23/09 05:02 PM
by houdinihar
* big inoculation problem Wonderboyz 537 7 07/30/08 07:03 PM
by aaronemerritt
* Setup pictures and Ideas With PIC's and inoculation problem with pic's thehappymushroom 539 6 01/15/09 01:43 PM
by tahoe
* inoculation problem sHrOoMeRrR420 481 5 12/02/04 12:47 PM
by sHrOoMeRrR420
* Inoculation problem? [IM PISSED] - added pics
( 1 2 all )
faqall 2,473 25 02/09/07 09:42 PM
by soulsizzle
* Rye Jar inoculation Problems lolwhut 776 9 07/17/07 01:02 AM
by Blutjager
* brf pftek inoculation problem 200proofhonky 273 2 08/05/08 07:05 PM
by 200proofhonky
* pan cyan inoculation problems santaclaws 1,030 11 04/22/07 07:55 PM
by usg543

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
617 topic views. 31 members, 179 guests and 51 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
SoulSpeciosa Kratom
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.01 seconds on 19 queries.