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Offlinespliffmasta
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Spore solution too dilute?
    #3317401 - 11/03/04 11:42 PM (13 years, 24 days ago)

I guess I don't really know what I was expecting. But i unloaded all of my spores into a sterilized gladware container with a flame heated and alcohol wiped knife. I wrapped 15 x 12cc syringes in foil and steam sterilized them for 1.5 hours then let them cool. I flamed each needle before injecting its water into the gladware then shook up the solution. I only injected about 120cc of water in there, but the solution is very dilute, they look as if they pretty much disolved. Did I spread my mix too thin? I asked earlier and Sam (very wise man) said that I could potentially do all 20 of the syringes, but to play it safe, and based on my batch of jars, 10 is plenty. I plan to flame each needle and wipe it with alcohol before I suck up the spores.

A) Am I ok on my solution?
B) Am I ok on my process and procedures?

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Edited by spliffmasta (11/03/04 11:44 PM)


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Invisibledobinky
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Re: Spore solution too dilute? [Re: spliffmasta]
    #3317438 - 11/03/04 11:48 PM (13 years, 24 days ago)

why not just suck up boiling water 3-4 times (squirt it out elsewhere) and let em cool...sterile syringes in just a few minutes


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Offlinespliffmasta
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Re: Spore solution too dilute? [Re: spliffmasta]
    #3317469 - 11/03/04 11:55 PM (13 years, 24 days ago)

lol, yea I did that too, before steaming them for 1.5 hours, I'm a loser, I just don't wanna ruin the single most important step, doin the overkill way, I just wanna know if this dilute stuff is aight! :smile:


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Invisiblederx
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Re: Spore solution too dilute? [Re: spliffmasta]
    #3318601 - 11/04/04 06:14 AM (13 years, 23 days ago)

a single spore is microscopic. If the spore solution is mixed up, then you're fine.


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InvisibleSam1912
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Re: Spore solution too dilute? [Re: derx]
    #3318609 - 11/04/04 06:22 AM (13 years, 23 days ago)

You'll be fine. That's a lot of spores you got there. Let it sit in the syringe for about a day(I waited 20 hours first time) for the spores to get rehydrated before inoculation.

Sam


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Invisiblegeorge castanzaM
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Re: Spore solution too dilute? [Re: Sam1912]
    #3319033 - 11/04/04 11:12 AM (13 years, 23 days ago)

time will tell..........


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OfflineHenry_Halleur
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Re: Spore solution too dilute? [Re: george castanza]
    #3319206 - 11/04/04 11:55 AM (13 years, 23 days ago)

mushmush says thats its possible to make up to 40 syringes out of one print.
i usually use one print for one syringe :wink:


Edited by Henry_Halleur (11/04/04 11:56 AM)


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Amazon Shop: ½ Pint Jars, Microscope

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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