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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Mexicana and Tampanensis

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InvisibleChampion des Champignons
long standing member;)

Registered: 07/27/00
Posts: 2,679
Loc: Alba
Re: mexicana won't pin [Re: ]
    #322822 - 05/21/01 10:25 PM (19 years, 7 months ago)

Thanks Teonan. I've never grow a sclerotium forming species before. I'm all excited!:D I was really just growing them for the fruits, and maybe prints if I'm lucky.
Is a sclerotium from a casing suitable for cloning, or will compost and shit stuck to it make it too prone to contams?

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Anonymous

Re: mexicana won't pin [Re: Champion des Champignons]
    #322831 - 05/21/01 10:37 PM (19 years, 7 months ago)

Just clean them real good with water before drying. I think it is the best way to store a STRAIN long term. I wish all species grew sclerotia. Remember you can always surface sterilize them later before cloning.

Edited by Teonan on 05/21/01 10:39 PM.



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OfflineTriple_3
member
Registered: 03/21/01
Posts: 99
Last seen: 19 years, 6 months
Re: mexicana won't pin [Re: ]
    #322840 - 05/21/01 10:46 PM (19 years, 7 months ago)

is sclerotia produced best in jars
or in casings?

seems like it would be more sterile in jars, no mushies though...

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Anonymous

Re: mexicana won't pin [Re: Triple_3]
    #322870 - 05/21/01 11:12 PM (19 years, 7 months ago)

Both P. mexicana and P. tampanensis form sclerotia invitro on ryegrass seed or in casing layers. No one on this BBoard has had any success with Invitro production with P. mexicana. Apparently not all spore races of P. mexicana form invitro sclerotia. Paul Stametes described growing P. mexicana sclerotia invitro, but he was using a strain that was known to produce. The spore race in circulation from one of the vendors here, does not do this. But it does produce heavily in a casing layer. This same spore vendor has P. tampanensis, and this species does both. It produces heavily invitro on rye grass seed, in the casing layer, and it fruits.
To answer your question, as of now, only the tampanensis produce invitro on ryegrass seed. But the mexicana produces exceptionally well in a casing layer.



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OfflineWorkmanV
Psilocybe Microscopist
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Re: mexicana won't pin [Re: ]
    #322957 - 05/22/01 12:59 AM (19 years, 7 months ago)

I think that is about to change. A new strain from a different collection has proven to be a superior invitro sclerotia producer. Well its not really new, I just didn't do as much initial work with it because it fruited so poorly. I guess you can blame it on SVS (Spore Vendor Syndrome), the tendency to select strains for their superior spore production at the expense of other more desirable traits.

For now I am calling it strain "A". There are pictures of it fruiting in the gallery and those large sclerotia in my hand are strain "A". I'll try to squeeze out a few syringes in a couple of weeks. If you are planning on growing the mexicana for invitro sclerotia, it might be worth the wait. I will make the distinction clear on the Spore Works site between the two strains when they are available. For now strain "B" fruits nicely or gives you casing sclerotia if your grow chamber isn't to its liking. Invitro production for strain "B" is typically rapid initially then soon stalls with sclerotia around rice grain sized.

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OfflineTriple_3
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Registered: 03/21/01
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Re: mexicana won't pin [Re: Workman]
    #322992 - 05/22/01 01:49 AM (19 years, 7 months ago)

thanx

can't wait...

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Invisiblesolidstate
journeyman
Registered: 04/09/00
Posts: 56
Re: mexicana won't pin [Re: Triple_3]
    #323200 - 05/22/01 12:01 PM (19 years, 7 months ago)

hmmm...friend said he was fanning 4x per day, misting 2-3x per day, around 75f, 4-8 hrs light per day (long involved story)...this has worked fine for his pan & cubensis experiments, what should he change to favor mexicana pin initiation? last I heard, he was now seeing a bunch of sclerotia on several strains but no pins...btw looking forward to the A strain Workman



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OfflineWorkmanV
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Re: mexicana won't pin [Re: solidstate]
    #323227 - 05/22/01 12:32 PM (19 years, 7 months ago)

That sounds like it should work. But sclerotia formation in the casing either means it is a poor fruiting strain or the conditions are not quite right and the mycelium is experiencing stress. It is kinda cool that even if conditions are not ideal you can still get a harvest of sorts. How long has your friend been waiting so far?

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Anonymous

Re: mexicana won't pin [Re: Workman]
    #323770 - 05/22/01 11:35 PM (19 years, 7 months ago)

I think it's genetics not environment.



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Invisiblesolidstate
journeyman
Registered: 04/09/00
Posts: 56
Re: mexicana won't pin [Re: ]
    #324098 - 05/23/01 12:18 PM (19 years, 7 months ago)

day 17, we are not alone...impatience is the workshop of the devil...friend reports that out of 8 isolated strains, some have produced a small number of large sclerotia, others a large amount of small sclerotia, and one a fair number of pins...lots of variability w/ this species...perhaps the lack of light slowed things down a bit



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InvisibleChampion des Champignons
long standing member;)

Registered: 07/27/00
Posts: 2,679
Loc: Alba
Re: mexicana won't pin [Re: solidstate]
    #327578 - 05/28/01 03:27 AM (19 years, 7 months ago)

Still no pins, but the spawned manure is producing plenty of fat sclerotia. What's the best way to go about cloning a good sclerotium producing strain? Agar I suppose?

------------------------------------
Old Donkeys don't die, they go to:
Drool Donkey Island


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hmmm........


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Invisiblepaddoholland
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Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: mexicana won't pin [Re: Champion des Champignons]
    #327675 - 05/28/01 08:29 AM (19 years, 7 months ago)

You must have a mexicana strain wich is grown from cloned sclerotia. When cloned several times (I heard) they won't form shrooms anymore.
I suggest you order a mexicana print from mush mush!!
Grow the spores from the print and you will have mexicana shrooms!!

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InvisibleChampion des Champignons
long standing member;)

Registered: 07/27/00
Posts: 2,679
Loc: Alba
Re: mexicana won't pin [Re: paddoholland]
    #328121 - 05/28/01 08:32 PM (19 years, 7 months ago)

In reply to:

You must have a mexicana strain wich is grown from cloned sclerotia.



I don't think so, it's Sporeworks "B" strain, which seems to produce fruit well enough. I'm obviously doing something wrong, though I can't work out what it is. Patience........

------------------------------------
Old Donkeys don't die, they go to:
Drool Donkey Island


--------------------
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hmmm........


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Invisiblepaddoholland
addict

Registered: 02/14/01
Posts: 317
Loc: the Netherlands
Re: mexicana won't pin [Re: Champion des Champignons]
    #328389 - 05/29/01 01:35 AM (19 years, 7 months ago)

Okay, your right, someone expert should know it!

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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: mexicana won't pin [Re: Champion des Champignons]
    #328454 - 05/29/01 02:58 AM (19 years, 7 months ago)

In my opinion your substrate is the problem. We never had any fruiting problems on grass seed (and boy can those mexicana fruit abundantly!. Other grains such as rye, millet and birdseed didn't produce any mushrooms.
I suggest you try the whole thing again with sterilised grass seed as a substrate.

check this post for some nice pics

Una


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Anonymous

Re: mexicana won't pin [Re: Una]
    #329273 - 05/30/01 12:17 AM (19 years, 7 months ago)

Growing them on rye grass seed, but only have one casing with TWO large pins and a whole lot of sclerotia. The other two casings only have sclerotia so far.



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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Mexicana and Tampanensis

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