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 Champion des Champignons
long standing member;)
Registered: 07/26/00
Posts: 2,679
Loc: Alba
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 Re: mexicana won't pin  [Re: ]
#322822 - 05/21/01 08:25 PM (21 years, 10 months ago) |
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Thanks Teonan. I've never grow a sclerotium forming species before. I'm all excited!:D I was really just growing them for the fruits, and maybe prints if I'm lucky.
Is a sclerotium from a casing suitable for cloning, or will compost and shit stuck to it make it too prone to contams?
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Old Donkeys don't die, they go to:
Drool Donkey Island
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hmmm........
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Anonymous
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Just clean them real good with water before drying. I think it is the best way to store a STRAIN long term. I wish all species grew sclerotia. Remember you can always surface sterilize them later before cloning.
Edited by Teonan on 05/21/01 10:39 PM.
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 Triple_3
member
Registered: 03/21/01
Posts: 99
Last seen: 21 years, 9 months
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Re: mexicana won't pin [Re: ]
#322840 - 05/21/01 08:46 PM (21 years, 10 months ago) |
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is sclerotia produced best in jars
or in casings?
seems like it would be more sterile in jars, no mushies though...
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Anonymous
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Re: mexicana won't pin [Re: Triple_3]
#322870 - 05/21/01 09:12 PM (21 years, 10 months ago) |
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Both P. mexicana and P. tampanensis form sclerotia invitro on ryegrass seed or in casing layers. No one on this BBoard has had any success with Invitro production with P. mexicana. Apparently not all spore races of P. mexicana form invitro sclerotia. Paul Stametes described growing P. mexicana sclerotia invitro, but he was using a strain that was known to produce. The spore race in circulation from one of the vendors here, does not do this. But it does produce heavily in a casing layer. This same spore vendor has P. tampanensis, and this species does both. It produces heavily invitro on rye grass seed, in the casing layer, and it fruits.
To answer your question, as of now, only the tampanensis produce invitro on ryegrass seed. But the mexicana produces exceptionally well in a casing layer.
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Workman
Psilocybe Microscopist
 
Registered: 03/01/01
Posts: 3,566
Loc: Washington, USA
Last seen: 4 years, 3 months
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Re: mexicana won't pin [Re: ]
#322957 - 05/21/01 10:59 PM (21 years, 10 months ago) |
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I think that is about to change. A new strain from a different collection has proven to be a superior invitro sclerotia producer. Well its not really new, I just didn't do as much initial work with it because it fruited so poorly. I guess you can blame it on SVS (Spore Vendor Syndrome), the tendency to select strains for their superior spore production at the expense of other more desirable traits.
For now I am calling it strain "A". There are pictures of it fruiting in the gallery and those large sclerotia in my hand are strain "A". I'll try to squeeze out a few syringes in a couple of weeks. If you are planning on growing the mexicana for invitro sclerotia, it might be worth the wait. I will make the distinction clear on the Spore Works site between the two strains when they are available. For now strain "B" fruits nicely or gives you casing sclerotia if your grow chamber isn't to its liking. Invitro production for strain "B" is typically rapid initially then soon stalls with sclerotia around rice grain sized.
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Triple_3
member
Registered: 03/21/01
Posts: 99
Last seen: 21 years, 9 months
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Re: mexicana won't pin [Re: Workman]
#322992 - 05/21/01 11:49 PM (21 years, 10 months ago) |
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thanx
can't wait...
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solidstate
journeyman
Registered: 04/08/00
Posts: 56
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Re: mexicana won't pin [Re: Triple_3]
#323200 - 05/22/01 10:01 AM (21 years, 10 months ago) |
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hmmm...friend said he was fanning 4x per day, misting 2-3x per day, around 75f, 4-8 hrs light per day (long involved story)...this has worked fine for his pan & cubensis experiments, what should he change to favor mexicana pin initiation? last I heard, he was now seeing a bunch of sclerotia on several strains but no pins...btw looking forward to the A strain Workman
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 3,566
Loc: Washington, USA
Last seen: 4 years, 3 months
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Re: mexicana won't pin [Re: solidstate]
#323227 - 05/22/01 10:32 AM (21 years, 10 months ago) |
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That sounds like it should work. But sclerotia formation in the casing either means it is a poor fruiting strain or the conditions are not quite right and the mycelium is experiencing stress. It is kinda cool that even if conditions are not ideal you can still get a harvest of sorts. How long has your friend been waiting so far?
The Spore Works
Exotic Spore Supply
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Anonymous
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Re: mexicana won't pin [Re: Workman]
#323770 - 05/22/01 09:35 PM (21 years, 10 months ago) |
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I think it's genetics not environment.
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solidstate
journeyman
Registered: 04/08/00
Posts: 56
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Re: mexicana won't pin [Re: ]
#324098 - 05/23/01 10:18 AM (21 years, 10 months ago) |
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day 17, we are not alone...impatience is the workshop of the devil...friend reports that out of 8 isolated strains, some have produced a small number of large sclerotia, others a large amount of small sclerotia, and one a fair number of pins...lots of variability w/ this species...perhaps the lack of light slowed things down a bit
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Champion des Champignons
long standing member;)
Registered: 07/26/00
Posts: 2,679
Loc: Alba
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Re: mexicana won't pin [Re: solidstate]
#327578 - 05/28/01 01:27 AM (21 years, 10 months ago) |
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Still no pins, but the spawned manure is producing plenty of fat sclerotia. What's the best way to go about cloning a good sclerotium producing strain? Agar I suppose?
------------------------------------
Old Donkeys don't die, they go to:
Drool Donkey Island
--------------------
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hmmm........
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paddoholland
addict
Registered: 02/13/01
Posts: 317
Loc: the Netherlands
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You must have a mexicana strain wich is grown from cloned sclerotia. When cloned several times (I heard) they won't form shrooms anymore.
I suggest you order a mexicana print from mush mush!!
Grow the spores from the print and you will have mexicana shrooms!!
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Champion des Champignons
long standing member;)
Registered: 07/26/00
Posts: 2,679
Loc: Alba
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In reply to:
You must have a mexicana strain wich is grown from cloned sclerotia.
I don't think so, it's Sporeworks "B" strain, which seems to produce fruit well enough. I'm obviously doing something wrong, though I can't work out what it is. Patience........
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Old Donkeys don't die, they go to:
Drool Donkey Island
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hmmm........
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paddoholland
addict
Registered: 02/13/01
Posts: 317
Loc: the Netherlands
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Okay, your right, someone expert should know it!
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First home-grown big mamma.
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Visit & Support Free Spore Ring Europe
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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In my opinion your substrate is the problem. We never had any fruiting problems on grass seed (and boy can those mexicana fruit abundantly!. Other grains such as rye, millet and birdseed didn't produce any mushrooms.
I suggest you try the whole thing again with sterilised grass seed as a substrate.
check this post for some nice pics
Una
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Anonymous
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Re: mexicana won't pin [Re: Una]
#329273 - 05/29/01 10:17 PM (21 years, 9 months ago) |
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Growing them on rye grass seed, but only have one casing with TWO large pins and a whole lot of sclerotia. The other two casings only have sclerotia so far.
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