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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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InvisibleMyco-420
enthusiast

Registered: 09/28/00
Posts: 66
Loc: BFE
Whats the best way to keep extra agar?
    #312250 - 05/08/01 10:48 AM (22 years, 4 months ago)

Just wondering because my friend hasa whole bunch extra.... freeze it?

Someone should email me a bowl.


--------------------
Bong hits for Jesus


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Offlinegray1
addict

Registered: 04/30/01
Posts: 430
Loc: brooklyn
Last seen: 21 years, 5 months
Re: Whats the best way to keep extra agar? [Re: Myco-420]
    #312265 - 05/08/01 11:13 AM (22 years, 4 months ago)

your friend has extra dyr (powder agar)?
it will keep indefinately at room temperature in an airtight container, agar is hygroscopic.
a cool, dry place would be best, the fridge is fine as long as the container is airtight, but not necessary.

i don't think that freezing agar that has already been melted into solution/poured to plates is a good idea.

c12h16n24ohdmt


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InvisibleMyco-420
enthusiast

Registered: 09/28/00
Posts: 66
Loc: BFE
Re: Whats the best way to keep extra agar? [Re: gray1]
    #312270 - 05/08/01 11:22 AM (22 years, 4 months ago)

NO i guess I should have been more clear.... it is PDA solution. Already made.

Someone should email me a bowl.


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Bong hits for Jesus


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Offlinegray1
addict

Registered: 04/30/01
Posts: 430
Loc: brooklyn
Last seen: 21 years, 5 months
Re: Whats the best way to keep extra agar? [Re: Myco-420]
    #312279 - 05/08/01 11:31 AM (22 years, 4 months ago)

if you have plates poured, they will keep in the fridge for several weeks, however, you need to keep an eye out for two things: contamination (especially if you use a home fridge that you also keep food in. those things are crawling with bacteria, yeast, and mold) and the plates/slants drying out.
if the solution is in a jar and hasn't been poured as of yet you don't have to be concerned with losing moisture as long as the container is capped tightly. you will have to melt/sterilize the solution again anyway in order to pour plates/make slants.

although i don't know of any specific reason not to, i don't think freezing sounds like a good idea. it may affect the ability of the agar to gel again in the future, and i have extensive experience in a microbiology lab and have never seen ort heard of anyone freezing agar to store it.



c12h16n24ohdmt


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Offlinepsylohassee
enthusiast
Registered: 11/15/00
Posts: 106
Last seen: 22 years, 3 months
Re: Whats the best way to keep extra agar? [Re: Myco-420]
    #312382 - 05/08/01 02:23 PM (22 years, 4 months ago)

My dads sisters moms sons son has kept agar in the fridge for months with no contam problems. Of course he always adds a bit of h202 after it cools, and when hes ready to use again, just pours it off. Doesnt ever harm the spores, and keeps real nice.



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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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