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Invisible40oz
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Registered: 01/19/01
Posts: 30,035
Loc: Sandy Eggo. Ca.
initiating pinning
    #308329 - 05/03/01 09:23 AM (15 years, 7 months ago)

i have treasure coasts that have been cased 1 week via 50/50+ tek, the mycelium has colonized the casing, not very thick though, ...no little pins.... just 2 big fat shrooms (2in. long)that have grown over 3 days.....what the hell is that?
should i pick these early birds?
and/or should i initiate pinning by a drop in temperature?
the temp presently is @ 78' degrees,
and the present humidity is @ 84%.

~fortyounces2freedom~ & ~disconnectedhaze~



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OfflineElektrolurch
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Registered: 05/02/00
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Re: initiating pinning [Re: 40oz]
    #308410 - 05/03/01 12:16 PM (15 years, 7 months ago)

Hi,

was it a multispore culture from a spore syringe? Multispore cultures contain a lot of different genotypes, some fruit earlier some later.
If there are already 2 mushrooms, then you are on the right path. 84 % humidity is a little bit too low. Maybe casing got dry?
Else, just wait, they'll come...

Elektrolurch



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Invisible40oz
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Registered: 01/19/01
Posts: 30,035
Loc: Sandy Eggo. Ca.
Re: initiating pinning [Re: Elektrolurch]
    #308689 - 05/03/01 06:54 PM (15 years, 7 months ago)

multispore culture?...i dunno....
i got the print from sporeworks and transferred it to syringe.
in the bio about the strain he said it was a quik colonizer,
i researched it @ other sources and found that not correct.
Treasure Coasts are the real true slow poke.
suggested humidity is 82%-92%, ill increase the humidity.
should i initiate pinning still?

~fortyounces2freedom~ & ~disconnectedhaze~



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tiny_rabid_birds said:
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OfflineTriple_3
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Re: initiating pinning [Re: 40oz]
    #309042 - 05/04/01 02:55 AM (15 years, 7 months ago)

anything innoculated with spores is called a multispore culture...
How long did it take to colonize?...I though they just took a long time to fruit....
yes keep initiating

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Invisible40oz
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Re: initiating pinning [Re: Triple_3]
    #309047 - 05/04/01 03:21 AM (15 years, 7 months ago)

they fuckn took forever!!!!!!!!
i innoc. at the same time, with the same substrate,
that i did with B+, 9 jars out of 12 went sour because they took too damn long...
put it this way....
the time it took for colonization;
the B+ had colonized, cased, flushed, dried & consumed by the time the remaining jars of the T.C were ready.

as for initiating pinning, how is the best way to do that?
i've heard to stick in in the fridge, im not going to do that!
i have an Ice Pac.....if i could incorperate that into the terrarium, which is the best way to do so...
i.e..wrap it in a washcloth & stick it under the casing ,
put on top of the perlite..etc...

this is the toughest p.c strain ive delt with.


~fortyounces2freedom~ & ~disconnectedhaze~


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tiny_rabid_birds said:
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Offlinesonichell
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Re: initiating pinning [Re: 40oz]
    #309063 - 05/04/01 04:04 AM (15 years, 7 months ago)

maybe drop to 74-76 F and also raise humidity to around 95% or so.



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Invisible40oz
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Re: initiating pinning [Re: sonichell]
    #309077 - 05/04/01 04:31 AM (15 years, 7 months ago)

these are casings, not cakes...

~fortyounces2freedom~ & ~disconnectedhaze~


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tiny_rabid_birds said:
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Offlinegratefulredhead
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Re: initiating pinning [Re: 40oz]
    #309156 - 05/04/01 09:48 AM (15 years, 7 months ago)

hey--i would suggest picking those early birds, having a little snack, raising the humidity to 95-100%, and making sure you have good air exchange. probably your temp. is fine.

if this fails after a week, you might try "deep scratching" with a steril instrument of choice, adding another shallow layer of casing, and giving it another try. be patient and don't give up!

here's some other details taken from various sources:

The three stages of casing maintenance...
1. First of all, while a casing incubates, they will be fine a terrerium, covered with saran wrap. For incubation, high humidity and little air exchange is desirable.
2. The next phase after the casing is colonized is pin initiation. During this stage, the casing needs light, fresh air, and high humidity. The higher the humidity, the higher the need for fresh air, and also the higher the risks of contaminates. But conversely (and likely a source of disagreement amongst teks) High humidity is absolutely neccesary at this point to achieve an optimum pin set (more pins/ more evenness of pin set=more shrooms). 95%-100% humidity is the goal for this stage. Most people probably don't regulate to higher than the 85% humidity required of the next stage for their pin initiation. They get results, but they rarely get the wall to wall pins that a propper pin set should produce) As soon as you seen pins, you move on the the next stage.
3. The next stage is fruitbody developement. At this stage you need lower humidity. 85% seems to be ideal, but never higher than 90%. Higher humidity increases the chances of contams, while diminishing the productiveness of the casing. You see, you need to have evaporation off of the casings surface, to pump water/nutrients into the shrooms via capilary action.




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Invisible40oz
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Re: initiating pinning [Re: gratefulredhead]
    #309345 - 05/04/01 03:13 PM (15 years, 7 months ago)

Thanks for going into detail, people like you are appreciated here.
lastnight, i stuck an icepac wrapped in a washcloth under neith the tray, and this morning i had alot of pins.
i dont know if it this method is affective, but to my sight, it was.
the humididty has been increase to 90%, i cant get it to go higher cuz the local home depot stopped carring perlite....
....fuck!
i have to go on a mission for more.
i installed a fan on a timer that sux the air out every few hours.
what would you suggest on what intervals this fan should be on/off and for how long?
these treasure coasts are a pain in the ass,
not like anyother strain ive delt with.

thanks again.



~fortyounces2freedom~ & ~disconnectedhaze~


--------------------
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tiny_rabid_birds said:
"your avatar is dirty."


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Offlinegratefulredhead
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Re: initiating pinning [Re: 40oz]
    #309365 - 05/04/01 03:32 PM (15 years, 7 months ago)

the ice pack seemed to help, eh? excellent.

remember that 3rd step there! when you have a good strong pin set, lower the ol' humidity. 90% or below... your fan should help with that issue.

best to you, once again... let us know how it works out.



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Invisible40oz
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Re: initiating pinning [Re: gratefulredhead]
    #309410 - 05/04/01 04:44 PM (15 years, 7 months ago)

thanks for your insight,
and about that fan....intervals for on/off time?



~fortyounces2freedom~ & ~disconnectedhaze~


--------------------
:pacman: - - - -  :pill: :mushroom2: :pill2: :mushroom2: :regularshroom: :mushroomgrow: :pill: :pill2: :mushroom2: :poison:

:sun::heart::sun:

tiny_rabid_birds said:
"your avatar is dirty."


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Offlinegratefulredhead
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Re: initiating pinning [Re: 40oz]
    #309510 - 05/04/01 07:06 PM (15 years, 7 months ago)

i have never used an electronic fan--i always have just opened the top and fanned. with that disclaimer, maybe you can set it to mimic diligent fanning by hand--set for 4 or 5 times a day for 15 minutes or so, depending on how much air it exchanges. but, as i said above--i'm just theorizing on that one... regardless, careful that it doesn't drop the humidty too far...

best...



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