Belome said:

what kind of pen do you guys use to write on your dishes?

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DV8DUG said:

Tri said:

Good idea, thanks for the tips I’ve been shocked by plates not contaminating after a fall. I think I do need to do the test you mentioned, I used to be a lot more confident with SAB work but battling so much contam trying to revive all my old plates has me on edge

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Why don't you just ask for some spores? No positive effects with bringing troubles to the show from the start.

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You’re very right, I think it’s a combination of being sentimental, wanting to overcome the adversity, and having a strong dislike for asking for help. I’m trying to be more self advocating, I even asked beef for SGT finally and I’ve been eyeing those beauties for 3 years

To be clear I have a small variety of spores, I’m not destitute it’s just all my cultures were nuked. It’s hard to find the balance though, working with them has definitely made me feel like a noob again but I’m battling 2 different white molds on top of trich and bacteria, it’s been frustrating but I believe I’ll be rewarded on the other side maybe

@Fonti I appreciate you I’m always down to trade but no concern necessary

awsomeguy20211 said:

So did my first agar transfer after letting some spores germinate, I believe some of the bacteria from the first plates has made it into my second plates. Theres a very fast spreading pink bacteria which I will dispose of. Then the other plates feature these small perfectly circular spots, Im assuming it is bacteria, but it grows a bit more slowly. Anyone know more? thus far only one plate shows no signs of contamination, I'm still getting used to working in a SAB!

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Deep Space said:

I tried cloning a Pan cyan recently for the first time and last night made some AM transfers from the T1 plates. I’ve never attempted an AM transfer either but i think it turned out pretty good. Pics were taken twenty four hours after the transfers. The plates are 90mm for reference, and three transfers were made to each plate. How these grow out over the next week will dictate how i proceed.

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fontinalis666 said:

How long do you usually let your plates sit unwrapped in the sab to deal with condensation? I did my last pour a lil hot and have some serious condensation in some of the plates, a couple even have some slight pooling on the agar, should I be overly concerned?

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I usually let them come to room temp, then wrap. With the cooler weather my place is around 17C, and even if I manage to avoid condensation on the lids I get it on the sides, to the point that it knocks onto the plate and makes a mess. These guys I poured really thin- you can't see it, but most of them have bare spots on the plate. And left them overnight, wrapped in the morning. We'll see how they do. I do like prepping plates at least a couple of weeks before use, to let condensation do its thing, and just be sure they're clean.

I don't really care if there's a bit of condensation, but water running around, that's a bitch. I turn them upside down before lifting the bottom to transfer, and that water is a massive vector.

fontinalis666 said:

How long do you usually let your plates sit unwrapped in the sab to deal with condensation? I did my last pour a lil hot and have some serious condensation in some of the plates, a couple even have some slight pooling on the agar, should I be overly concerned?

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I wouldn't be. All things being equal the condensation will eventually evaporate and the pooling water will be sterile if all precautions were taken. Let the agar absorb what's pooling and wrap them when you feel the condensation has dissipated enough. Pouring hot happens, but no need to panic if you did everything else the right way.