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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Deep Space] * 3
    #29725834 -



Trying out condiment cups, this felt like such a crapshoot if I’m being honest :p

Flipping the cup over and pouring and having it open while grabbing the lid from the stack felt like being naked public :lol: vulnerable as heck. We’ll see how many contaminate guess

Any tips?


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𝕴𝖋 𝕴 π–π–†π–›π–Š π–˜π–Šπ–Šπ–“ π–‹π–šπ–—π–™π–π–Šπ–— π–Žπ–™ π–Žπ–˜ π–‡π–ž π–˜π–™π–†π–“π–‰π–Žπ–“π–Œ π–šπ–•π–”π–“ π–™π–π–Š π–˜π–π–”π–šπ–‘π–‰π–Šπ–—π–˜ 𝖔𝖋 π•²π–Žπ–†π–“π–™π–˜

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri] * 2
    #29725875 -

I’d go with shorter wider cups, I use 3.25oz ones.

Cups are Lips facing down, sacrifice the bottom one. Same with the lids, keep the part that is exposed to the air after be the only thing exposed to the air in the sab. Like this



They are surprisingly good. My attempts at regular plates were disastrous in comparison.

I do around 70 at once with 1000mls. Try keeping one poured and open for a while in the sab while you pour more, and see if it even contaminates.

That was a nice test for me, showed me that overall my technique is good and isn’t quite a vector.

Also air tight lids rule


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I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Edited by Huskies (11/13/26 02:39 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies] * 1
    #29725937 -

Good idea, thanks for the tips :cool: I’ve been shocked by plates not contaminating after a fall. I think I do need to do the test you mentioned, I used to be a lot more confident with SAB work but battling so much contam trying to revive all my old plates has me on edge :lol:


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𝕴𝖋 𝕴 π–π–†π–›π–Š π–˜π–Šπ–Šπ–“ π–‹π–šπ–—π–™π–π–Šπ–— π–Žπ–™ π–Žπ–˜ π–‡π–ž π–˜π–™π–†π–“π–‰π–Žπ–“π–Œ π–šπ–•π–”π–“ π–™π–π–Š π–˜π–π–”π–šπ–‘π–‰π–Šπ–—π–˜ 𝖔𝖋 π•²π–Žπ–†π–“π–™π–˜

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri] * 5
    #29726072 -

P. semperviva hvc T2 transfers going to grain today.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri] * 1
    #29726148 -

Tri said:
Good idea, thanks for the tips :cool: I’ve been shocked by plates not contaminating after a fall. I think I do need to do the test you mentioned, I used to be a lot more confident with SAB work but battling so much contam trying to revive all my old plates has me on edge :lol:


Why don't you just ask for some spores? No positive effects with bringing troubles to the show from the start.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DV8DUG] * 1
    #29726190 -

Imma send you a lil something back Tri :kiss:


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Re: AGAR ENVY! (Anything and All things agar!) [Re: fontinalis666]
    #29726257 -

what kind of pen do you guys use to write on your dishes?


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Ack! It's a snack attack!
And YOU'RE it!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Belome] * 1
    #29726263 -

Sharpies, fine point ones, but mine are sauce containers, I imagine regular plates work as well.

I get blank label stickers at dollarama for jar lids and such. Paper tape also seems really good, survives the PC, the ink seems to leave the paper tape, and it stays good.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Belome]
    #29726286 -

Belome said:
what kind of pen do you guys use to write on your dishes?


Paint markers. Once fully dried you can wipe vigorously with iso and it won’t shift it. Just don’t let the iso soak on it otherwise it’ll start to dissolve. I found that the hard way.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Deep Space] * 4
    #29726418 -

Ochras transfers I made 8 days ago. 60mm plates

This looks good to send to me, its my first transfer but the original plate I got was given to me by this amazing grower and im sure shes done her part to achieve such pretty mycelium.






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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 1
    #29726442 -

beauty! a quart per plate is the plan?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies]
    #29726459 -

I wasnt too sure. This will be my first time making my own grain (whole white oats) so I was probably going to ask or go with my instinct. 1 Plate per quart Jar sounds good, maybe a bit too much but if anything that should mean faster colonization.

I will make more transfers from the best looking plates, I only take a tiny amount per transfer and then send them to grain.

Exciting


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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 1
    #29726473 -

Yeah whoever hooked you up already seems to have done an amazing iso, and the growth all looks uniform.

Maybe try tiger dropping, which is cutting up the agar with a tic tac toe grid, so you have more inoculation points. The more agar you use, the higher the contam vector if it isn't clean (yours looks flawless) but the faster the colonization.

But yeah, your plan is solid. Get best plates onto new ones, but all of those look good to go for grains, one quart each IMO.

Maybe save one for doing LI? Basically put it in a jar of sterilized water, shake it up, and bam, mycelium suspended in water ready to go.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies]
    #29726503 -

Thanks man, appreciate the tips.

I could do LC to be honest... I got LME, small jars, distilled water, SHIP and hydrophobic filters so, why not?

Yeah this grower has some amazing cultures, shes boss.

May get into the grain today. A bit uncertain cos first timer but I know I can get it right. Just not a fan of having my loud PC running for 90-120 minutes but oh well... gotta do it if I want to move past the infamous Tek I have been doing until now.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 2
    #29726563 -

Don't bother with LC, I mean you can, but try just LI, liquid innoculant.

LC has a chance to contaminate because it is nutritious, but LI is sterile water + perfect plate, so less room for mold to get a hold and grow. If a mold spore goes into LI when you transfer, it's not gonna be able to colonize the agar that is already colonized with strong mycelium.

But in a LC, it will secretly plot its final plan and you won't be able to tell.

I let the agar in the jar for a few days, shaking around, just to create more strands of macroscopic mycelium to make sure it's saturated enough. All the benefits, no colonization time.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies] * 1
    #29726565 -

Solid, I will look into it. Thanks again mate


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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 1
    #29726631 -

Beautiful plates 0_tonin!
I agree with Huskies, go with LI, you'll get more inoculation points and use less plates:awesomenod:

I finally got my new plates, +50 lesgoooo:rockon:
Can only fit 8 in my PC tho, went with 2 drops of blue and drop of green with this one


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Some say we'll see Armageddon soon
I certainly hope we will
:madpirate:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Reishi Uchiha] * 2
    #29726662 -

^nice coloring! Are those standard sauce containers? I think mine have pp5 lids, but I've never tried actually pre-pouring them and pcing them.

Have you tried doing pours? I use three small bottles in an Instant Pot, totalling 1000ml.

That way I can do 70ish plates.

They are basically sterile from manufacturer, because they are immediately sleeved after they were basically molten plastic. I just open mind in the sab, and that has not been a contamination vector.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Huskies]
    #29726691 -

Thank you:hatsoff:
Haven't done pours, and I won't till I have flowhood. I just don't wanna risk it with setup I have.

Both cups and lids are pp5 but I don't trust them being sterile. These were called slime cups or something like that, not food(sauce) ones. Also cups and lids were in the same sleeve.
I appreciate the suggestion bro:hug:


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Some say we'll see Armageddon soon
I certainly hope we will
:madpirate:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Reishi Uchiha]
    #29726852 -

So did my first agar transfer after letting some spores germinate, I believe some of the bacteria from the first plates has made it into my second plates. Theres a very fast spreading pink bacteria which I will dispose of. Then the other plates feature these small perfectly circular spots, Im assuming it is bacteria, but it grows a bit more slowly. Anyone know more? thus far only one plate shows no signs of contamination, I'm still getting used to working in a SAB!







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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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