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krill
absolute gonzo


Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 6 months
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Potatoe Dextrose or Malt (agar)?
#5281582 - 02/09/06 12:22 PM (17 years, 11 months ago) |
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DAN QUAYLE!!!
so i have ONE sample ONE premade, sterile, sealed potato dextrose agar plate. i plan on getting MANY more. but which is better for isolating strains/cloning tissue? potato dextrose agar OR malt agar???
my first go at this is going to be an attempt to isolate sub-strains from the Treasue Coast cube. i want a clone of an awesome TC they also put off an albino tc substrain and also an orange substrain?
mmm... sounds like fun
-------------------- "DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson NO LEFT TURN UN-STONED
Edited by krill (02/09/06 12:23 PM)
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Snaggletooth
Stranger in a Strange Land


Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
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Re: Potatoe Dextrose or Malt (agar)? [Re: krill]
#5281671 - 02/09/06 12:44 PM (17 years, 11 months ago) |
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It doesn't really matter, I don't think one is better then the other. Though if you were to use only one or the other generation after generation then there could be strain degeneration I believe.
But I don't think you will have to worry about that.
--------------------
Atheist Chat
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Potatoe Dextrose or Malt (agar)? [Re: krill]
#5282494 - 02/09/06 04:33 PM (17 years, 11 months ago) |
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shift back and forth...using them both.
you don't want the mycelium getting used to one agar medium.
example...
1st plate is MEA..let that grow out...
then take a wedge from that and transfer to plate #2.
2nd plate is PDYA..let that grow out...
then take a wedge from that and tansfer to plate #3.
3rd plate would be back to MEA again.
continue on if need b...til you get a nice aggressive growth of mycelium.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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RoachMan
Old Man



Registered: 03/06/05
Posts: 2,083
Loc: Midwest
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Re: Potatoe Dextrose or Malt (agar)? [Re: Snaggletooth]
#6508929 - 01/29/07 04:34 PM (17 years, 3 days ago) |
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:BUMP:
I read the term "senescence" the other day (as related to mycology). I looked up the term and it refers to "growth cycle" of a plant.
Could you give me the mycological definition of "senescence", please?
I'm just guessing...that when a culture reaches senescence it will no longer grow when placed on a new plate of the same type of agar?
-------------------- "The mistake that I make is to try to come awake in a place you're just supposed to get shit faced or baked."
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