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Offlinekrill
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Registered: 10/25/05
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Potatoe Dextrose or Malt (agar)?
    #5281582 - 02/09/06 12:22 PM (17 years, 11 months ago)

DAN QUAYLE!!!

so i have ONE sample
ONE premade, sterile, sealed potato dextrose agar plate.
i plan on getting MANY more.
but which is better for isolating strains/cloning tissue?
potato dextrose agar OR  malt agar???

my first go at this is going to be an attempt to isolate sub-strains from the Treasue Coast cube.
i want a clone of an awesome TC
they also put off an albino tc substrain
and also an orange substrain?

mmm... sounds like fun :syringe:


--------------------
"DO NOT ADJUST YOUR MIND.  IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson

NO LEFT TURN UN-STONED


Edited by krill (02/09/06 12:23 PM)


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OfflineSnaggletooth
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Registered: 10/24/05
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Re: Potatoe Dextrose or Malt (agar)? [Re: krill]
    #5281671 - 02/09/06 12:44 PM (17 years, 11 months ago)

It doesn't really matter, I don't think one is better then the other. Though if you were to use only one or the other generation after generation then there could be strain degeneration I believe.

But I don't think you will have to worry about that.


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Atheist Chat


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InvisibleRoadkillM
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Potatoe Dextrose or Malt (agar)? [Re: krill]
    #5282494 - 02/09/06 04:33 PM (17 years, 11 months ago)

shift back and forth...using them both.

you don't want the mycelium getting used to one agar medium.


example...

1st plate is MEA..let that grow out...

then take a wedge from that and transfer to plate #2.

2nd plate is PDYA..let that grow out...

then take a wedge from that and tansfer to plate #3.

3rd plate would be back to MEA again.


continue on if need b...til you get a nice aggressive growth of mycelium.



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleRoachMan
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Posts: 2,083
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Re: Potatoe Dextrose or Malt (agar)? [Re: Snaggletooth]
    #6508929 - 01/29/07 04:34 PM (17 years, 3 days ago)

:BUMP:

I read the term "senescence" the other day (as related to mycology).
I looked up the term and it refers to "growth cycle" of a plant.

Could you give me the mycological definition of "senescence", please?

I'm just guessing...that when a culture reaches senescence it will no longer grow when placed on a new plate of the same type of agar?


--------------------
"The mistake that I make is to try to come awake in a place you're just supposed to get shit faced or baked."


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