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Liquid Culture Questions - Clear LC's vs Dark, Cloudy LC's (more complex nutrients)
    #29639668 -

I just ordered some LC's from a major online genetics company (can't list apparently) and I noticed they are really dark - my Oak Ridge syringes are almost dark brown and the Amazon LC's are a little lighter but still a dark brownish-greenish color. All the LC's I've worked with up to now were the clear liquid cultures that looked like they were raised in something like a simple peptone/dextrose/LME nutrient solutinon. Will I be able to turn 1 syringe of these into 30 syringes like I've been doing with the clear LC's? I have seen many growers with dark colored liquid culture jars, and I'd really like to know what kind of nutrients they are using. The company with the dark colored LC's included a card that explained that their cultures were much darker than most people's because they used a much more sophisticated nutrient solution, but they didn't provide any specific details. I believe it's legit because the mycelium inside is REALLY thick and healthy. I'm just concerned as to whether I'll be able to use a dextrose/soy peptone/light malt extract solution like I have been using with my other LC's and having great results. I literally just bought 3 pounds of LME and dextrose and some soy peptone to mix my own LC premix with so I'm gonna be bummed if I can't use this with these. I'm pretty sure I can but before I blow $100 in liquid cultures please let me know if these are going to be viable.

Also, I have a jar of GT from 7/19 that I was scared to use because it had started settling to the bottom and looked like it had more sediment on the bottom of the jar - the same way the only only liquid culture jar I've ever had fail went. I'm debating whether I should bother making more liquid culture jars from first generation liquid culture jars I made from liquid culture syringes I bought online. The only time I had a jar fail, it was a 2nd gen jar of Tidal Wive LC's that I had taken from the first jar I made from a syringe. I've read that genetics lose potency and by the 5th generation or so the genetics are extremely weak. And since I don't know what the companies I'm buying it from are doing, I'm wondering how many times I can make liquid cultures from liquid cultures before the genetics are total trash. I'm guessing more than a couple times is pretty risky. I'll post pics of the jar in just a second. The main thing I want to know is just if I am okay using my usual peptone/dextrose/LME liquid cultures with these True Blue LC syringes to multiply them?


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Still trying to find my way out of interzone.


Edited by bruiser9811 (08/04/26 03:40 AM)

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Re: Liquid Culture Questions - True Blue LC's [Re: bruiser9811] * 1
    #29639738 -

Just a quick note that you need to remove the vendor names in your post according to the posting rules.


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Re: Liquid Culture Questions - True Blue LC's [Re: bluescreen]
    #29639768 -

Shoot, sorry! Will do.


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Still trying to find my way out of interzone.


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Re: Liquid Culture Questions - True Blue LC's [Re: bruiser9811]
    #29639778 -

LME should be fine. The mixes aren't as perfectly uncolored, you can sometimes get some sediment or darkening after cooking.

Clarity matters more than sediment or color. Some ragged medium brown flakes at the bottom of an othewise crystal clear yellow mix can be okay, same can happen in agar if it gets a bit burnt.

This is light malt, I don't recall what % but probably 2-4%.



The first pic (and right side on the comparison) is clean. The left pic is bacterial af. Notice how much cloudier it is. BUT there is also a gigantic brick of myc in there. Third pic is the bacterial jar after giving it a bit of a swish. The ragged bits of sediment aren't a concern, the general cloudiness is. Both of these were tested on plates when I noc'd the LC jars and the bacterial showed up there too. Always good to double check.

My darkest ever mix came from soaking clean alder chips (for smoking meat, yellow bag) in water in a bucket for some weeks, and then using that like grain water in addition to the regular agar mix (so very high nute). I made it for p cyans but even the cubes exploded on it. Tried it again a year later in the same bucket and every jar failed to colonize. I think some nasty non-organism (chemicals etc) must have gotten in the bucket. Anyway, my guess is their LC is enriched with stuff like that. Varied diet etc.


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.~`  𝔅 𝔞 𝔤 𝔢 𝔩  🥯  𝔅 𝔦 𝔱 𝔢 𝔰  `~.
:salute: CAKE WARS III STARTS SEPTEMBER 1st :salute:

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Re: Liquid Culture Questions - True Blue LC's [Re: Rasmodius]
    #29639780 -

All the LC's I multiplied from clear liquid culture syringes have done great...part of my problem
Rasmodius said:
LME should be fine. The mixes aren't as perfectly uncolored, you can sometimes get some sediment or darkening after cooking.

Clarity matters more than sediment or color. Some ragged medium brown flakes at the bottom of an othewise crystal clear yellow mix can be okay, same can happen in agar if it gets a bit burnt.

This is light malt, I don't recall what % but probably 2-4%.



The first pic (and right side on the comparison) is clean. The left pic is bacterial af. Notice how much cloudier it is. BUT there is also a gigantic brick of myc in there. Third pic is the bacterial jar after giving it a bit of a swish. The ragged bits of sediment aren't a concern, the general cloudiness is. Both of these were tested on plates when I noc'd the LC jars and the bacterial showed up there too. Always good to double check.

My darkest ever mix came from soaking clean alder chips (for smoking meat, yellow bag) in water in a bucket for some weeks, and then using that like grain water in addition to the regular agar mix (so very high nute). I made it for p cyans but even the cubes exploded on it. Tried it again a year later in the same bucket and every jar failed to colonize. I think some nasty non-organism (chemicals etc) must have gotten in the bucket. Anyway, my guess is their LC is enriched with stuff like that. Varied diet etc.



All the LC's I multiplied from clear liquid culture syringes have done great...part of my problem and why I posted this is because these new LC's are SO DARK that even adding 5cc is going to darken the entire solution. I found this pic on google images, looks a lot like what I'm working with.

Claude and Google AI are telling me it will be fine growing these in an LME solution, but I'm kind of bummed that I'm not going to have perfectly clear liquid cultures that are easy to tell if contaminated just by sight.


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Still trying to find my way out of interzone.


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