Dave Bennett said:

The thread is titled "Would love to hear opinions about this weird grow pattern in transfers." Opinions require explanations and evidence to back them up and that's what I've provided. And it wasn't that complicated or difficult to understand.

Some people are happy to hone their cultivation skills and master Teks and have no interest in how the Teks or the mycelium works and that's perfectly OK. Others want to know everything they can find out about mycology, especially as it relates to cultivation and improve their knowledge and that's OK too. One isn't better than the other because it's all down to personal interest.

DV8DUG, what have I said that makes you think I don't know shit about genetics? What have I've stated that you think is incorrect?

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...and some of the genes that are expressed in reproductive mycelium are thought to stay "switched on" when the transfer grows out again on agar. So it's a well observed phenomenon that a clone culture from a fruit will fruit quicker than it's parent spore derived mycelium, for example, because a lot of the reproductive genes from the hyphae in the fruit stay switched on.

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PuffM4gicDr4gon said:

I guess what I'm saying is that if it's a bacterial Halo the Q-tip should pick some of that up and transfer it. If it's only digestive enzymes nothing should transfer or maybe a single cell or two will grow. If it's microscopic running mycelium then the Q-tip will hopefully grab some of that and transfer it.

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BeefExtreme said:

Bud, honestly your plates pretty much look fine.  Any grower with their salt will tell you that it's obviously good myc.  My only advice is to get into some regular plates so you can see what's really going on but frankly bud?

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My experience is that it does all kinds of weird shit and you don't exactly need an explanation for every single little thing. Tried and true processes combined with experience is what keeps the whole world medicated...

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While I tend to agree on this, this is the first time I see stuff like that, I mean it's not just some randomly uneven mycelium, look at that!



Anyway, thank you so much for sharing your thoughts. <3

RockinRobot said:

It has been my experience that changes in temperature can cause the halos in a culture. Faster/slower rate of growth as temp swings. Halo's do not immediately spell contamination.

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DV8DUG said:

That does nothing to address this argument. He isn't even talking about Psilocybe Cubensis.

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Coprinopsis cinerea is a model organism in fungal research and many biological functions and behaviours are conserved right across the whole fungal kingdom even down to yeasts.

The observation of earlier fruiting from cloned material has been discussed a lot in various groups and it worked when I tried it with cubes. And it's widely known that you can invigorate a tired isolated culture by cloning a fruit. Try it yourself. It's easy to set up a side by side experiment.

And the pics of the angel wing clone cultures I posted above shows clear as day that the mycelium grows out in several different growth patterns from the same sample of fruit. That's because different genes are being expressed depending on the function of the hyphae within the fruit.

And you can see it here. The spawn cakes either side were taken from the top and bottom of the stipe and the one in the middle from the cap near the gills of the same fruit. But as you can see the cap culture in the middle is still producing psilocybin and the stipe cultures aren't.

jee3apha said:

here's what I think, correct me if I'm reaching the wrong conclusions:

• the halo is not bacterial contamination because it's made of filaments;
  
• the halo could be some kind of fungi that coexists with the good mycelium;
  
• the halo could be part of the good mycelium that for some reason has that weird appearance.
  

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This is my understanding as well.

Some pics of the plate that's like this, with what's definitely cube myc (unknown yet if di or mono) atop this other thing (which per above might also be cube myc).


Here it is intersecting with bacteria. The halo is interrupted and seems to be on the same ground plane as the bacteria, while the other cube myc is growing overtop of the bacteria. IDK if that means anything.



Re sending it, trenchhing, swabbing, etc, going to do all of that.