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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: andymc] * 3
    #29556177 -

Most of my attempts haven't worked out.  But one has, so far.

A Thai Lipa Yai print (that I made) dated 08/12/12 appears to have germinated in a polysorbate spore syringe.

I fucked up the original LC I made with it and don't trust it, so I'm going to try starting a new one tonight.  But some myc-looking shit is definitely alive here.  I just hope it's the right shit.


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How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: andymc] * 2
    #29560289 -

I've got another one that has germinated something in the syringe

A PESH print (again, that I made) dated 25/10/10 - oldest one yet

I've been making spore syringes and then dropping the spores on agar after a month or two - none of these have germinated on agar.  But in the syringes, some of them seem to come alive.


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How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: andymc]
    #29574112 -

i have a old print on a paper, which failed to germinate for 3weeks. i am trying these out.

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: edboy]
    #29598045 -

🤔 interesting I might try some experimentation with this later this year (maby seeing if it might increase germination rates or speeds with different substrates with controls to compare to on small scale kinda 😶‍🌫️


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An enigma some caerulescens n a mono 😎

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: bloodssonnew] * 4
    #29608623 -

Ochra reviving in tween 20 and distilled water. 3+ year old spore print that would not germinate on agar.

I also have White Teacher (3+yr), Big Mac (3yr), and 2 varieties of Zaps (1&1/2yr) and 6 months going.

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: JW123] * 1
    #29618777 -

:rockon:

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Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: Stipe-n Cap] * 2
    #29618938 -



While we're bumped, here's a microscope pic showing the bits of surfactant among ochra spores. This is about a 1 drop tween 20 / ~400ml solution (most of a pint jar), after the mixture + spores went through a cheap vortexer in a ~2ml cryotube.

IDK what to make of it, if anything, but I hadn't seen a similar picture.

I can see how this would be useful for breaking up spore clusters, if it's reducing friction and dislodging clumps under the continuous directional force of a vortexer.

No idea how any of the hydration / revival stuff would work. Is it related to what's seen here (with ~3um particles intermixing with the spores physically) or is there something much smaller happening in terms of cell wall porosity etc.

No idea :shrug:

Would be curious to see experiments with both the amount of tween20 per volume, and whether or not there's agitation (and vs an equivalent mixture with only the sorbitol).

It'd be funny if it turned out the particles are small and non-sticky enough that agitation caused them to act as an abrasive, scratching away the exterior of the spore and exposing a more receptive surface. But I pulled that theory directly out of my ass, and if people are getting results that don't involve agitation, then it's probably something else.


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.~`  𝔅 𝔞 𝔤 𝔢 𝔩  🥯  𝔅 𝔦 𝔱 𝔢 𝔰  `~.
:salute: CAKE WARS III HAPPENING NOW :salute:

🧪 Castellani Preservation | 🔬 The Slidewich | ☠️ Touchless skull sterilizer | 📸 Agar plate scanner


Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: Stipe-n Cap] * 2
    #29632652 -

Got some ordered finally.  Got a bunch of old prints to experiment with.


-Kingboomer


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I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: kingboomer] * 1
    #29632811 -

Funny coming across this as my RustyWhyte print front 2021 refuses to germinate. :takingnotes: Looks like I wasn't alone in that experience

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: Stipe-n Cap] * 4
    #29659254 -



Figured I'd wake this thread back up.  Used 1 drop per 100ml tween 20 to hydrate some 4ish year old prints I'm using for the latest cake wars thread. These soaked a week before being used, and there wasn't much left of the prints.  Put a few drops to some pf pucks to check growth.  Fingers crossed 🤞


-Kingboomer


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: kingboomer] * 5
    #29666603 -

After about 6 months of trying to revive swabs of big mac I let a large portion of a swab float around in tween water for a couple of months. The first plate I made from that tuft of swab germinated and I took 3 transfers from that last week. Here is that germination plate today.

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: JW123] * 1
    #29666696 -

:vibin:

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: JW123]
    #29667053 -

JW123 said:
After about 6 months of trying to revive swabs of big mac I let a large portion of a swab float around in tween water for a couple of months. The first plate I made from that tuft of swab germinated and I took 3 transfers from that last week. Here is that germination plate today.



Glad to see this working for people.

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: Stipe-n Cap]
    #29667088 -

Mind if I ask did u have the tween solution in a jar or something in front of a sab or in a flow hood and did u pressure cook the tween 20? Or what's the process?:rockon:


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An enigma some caerulescens n a mono 😎

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: bloodssonnew]
    #29667286 -

Watch the YT video in the OP.

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: Stipe-n Cap] * 3
    #29669169 -

Success!

BHT:



CG:



Using pucks because I had em on hand from the cake wars thread.  15 days from noc, these soaked about a week.  Definitely adding this to my arsenal!


-Kingboomer


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

Extras: Filter Print Post Top
Re: Spore hydration/revival using Polysorbate 20 (Tween 20) [Re: kingboomer]
    #29674500 -

5 days later:

CG


BHT



The BHT got a little more than the CG did but at any rate I'm liking the tween 20!


-Kingboomer


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

Extras: Filter Print Post Top
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