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Re: Official Copelandia/Panaeolus Thread [Re: bongoman]
    #29502735 -

I would suggest transferring a pretty large piece to a fresh plate.  And I would probably put both the transfer and the receiving plate in the refrigerator for good measure.

If you have water agar prepped that's another option.  I've revived two month old mycelium off water agar with a transfer to a nutrient plate.  I don't know the limits of this but will be doing a three month transfer soon.  No fridge needed for the water agar.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 5
    #29502899 -

Panaeolus cyanescens make stones in my current AIO jar recipe.  This is one of a few jars doing it, but the other ones fruited and I this one didn't want too.  So I got curious and tore it apart.  Maybe the biochar or a combination of it and grass seed etc.  But I can imagine the char upregulating sclerotia production to protect against possible fire.




Maybe I'll make some popcorn jars with a bit of char and grass seed to see how they do.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29503066 -

Super cool! Do you think it’s active?

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Re: Official Copelandia/Panaeolus Thread [Re: AstroSporeFiend] * 1
    #29503147 -

The mycelium is active.  I've eaten colonized popcorn before though it's sketchy to do that because of bacteria.

So I'm sure they are active.  But those are mixed with substrate and there's not very many of them anyway.  They do smell like tamp stones though.

I'm trying to clone the biggest one on water agar.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29503188 -

What's your verdict peeps?





1- Tampanensis 1.5L
2- Eungai 1L
3- Komodo X ttbvi 1L

All noc'd on the 16th with agar, slipped a quarter of a plate into each on the 18th (I had put far too little). Shaken on the 25th. It's awfully quick but I've not done AIO's before so :shrug:

They were jamming on the 25th when I shook em and they've recovered beautifully. They're not cubes so I'm not expecting a lot of regrowth but looks good to me. No smells.

Psuedo case em' today?

Oh and Eungai grain 1L, same schedule.



I mean I'm pretty certain that's good to go too, but a 👍 is still life-affirming.

Good morning boys and girls - today's word is: Discombobulated


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #29503243 -

That looks very serious Tree, my cinctulus never made anything larger than 2x3 mm. Are you going to clone them to see if you can encourage them to make even more?


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Official Copelandia/Panaeolus Thread [Re: Churpee Choozle] * 1
    #29503260 -

It's not dead but so slow. Might try some yeast agar to see if that wakes it up


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Re: Official Copelandia/Panaeolus Thread [Re: conz0r]
    #29503292 -

It can be hard to tell from a picture.  I like to look at the top, if it's covered with mycelium and the rest of the container has lightened then yeah.

@churp yeah, I took some tissue from the big one to water agar.  This might be a mono and if so I'll clone one of the other cultures doing it and see if I can cross it.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29503351 -

Re: sclerotia

Posting that's here so we aren't having the conversation in the pictures thread.

https://davidmoore.org.uk/21st_century_guidebook_to_fungi_platinum/Ch09_08.htm


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29503360 -

Quote:
The term sclerotium is, therefore, a functional one that encompasses a variety of structures. The different forms arose by convergent evolution


That feels better to me.

Thanks


(also, i liked learning the term Oidia)

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Re: Official Copelandia/Panaeolus Thread [Re: Generic] * 4
    #29503369 -

I started some biochar out of my new bag last night.  Mixed it with water, jackpot, liquid nutrients, and worm castings.  If it starts to grow mold I will pasteurize it. Otherwise it's going to get sterilized anyway for AIO prior to use.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 3
    #29503407 -

Interesting stuff! If you decide to assay them, I'd be very interested in the outcome.

And the bispo EF cakes. Almost there.


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Official Copelandia/Panaeolus Thread [Re: Churpee Choozle] * 1
    #29503426 -

If I manage to get cleaner ones and enough to eat I'll let everyone know how it goes.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29503489 -

Didn’t know this thread existed! :shrug:

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Re: Official Copelandia/Panaeolus Thread [Re: AZ Starman2112] * 2
    #29503524 -

Happy to see you finally made it!

Been playing with ITS data and ncbi blast/phylogeny.fr

Edit:  IND01 is under the edit box in the first image...  whoops.





Nothing definitive since I'm still learning.  But a Cambo Pollock described is a cyan according to this and a recent paper (I'm leaning towards it not being an obsolete species name based on this and some other things).  And Pan India seems quite a bit distant from the rest.  Most of the ITS sequences were ran by the same lab.

I imagine I'll be spending a lot of time with Pan India this year, and sharing prints or swabs as I'm able :smile:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (03/02/26 12:56 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Churpee Choozle] * 1
    #29503538 -

Churpee Choozle said:
Interesting stuff! If you decide to assay them, I'd be very interested in the outcome.

And the bispo EF cakes. Almost there.



beautiful pompons! :hug:
these look great , whats the substrate?


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Copelandia/Panaeolus Thread [Re: chris77] * 3
    #29503561 -

Thanks Chris!
It's EF, so an outer shell as casing and the actual sub on the inside. The casing is coir, peat and calcium (and some verm as it was too wet). Filled with 40-30-30 horse dung, coir and millet.
Inoculated with wedges.


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Official Copelandia/Panaeolus Thread [Re: Churpee Choozle]
    #29503589 -

I have not done that so far. will check it out. curious to see more :smile:


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Copelandia/Panaeolus Thread [Re: Churpee Choozle] * 1
    #29503783 -

Churpee Choozle said:
Thanks Chris!
It's EF, so an outer shell as casing and the actual sub on the inside. The casing is coir, peat and calcium (and some verm as it was too wet). Filled with 40-30-30 horse dung, coir and millet.
Inoculated with wedges.


Nice! How long do you expect them to live in the jars? I've never done EF.



Curious if these look fine, doomed, or top grow. The similarly 'wet' TTBVI jara I posted a week or so ago ended up smelling and looking fine, and is what is colonizing fine on bulk in the CV+ thread. Cubes in the same batch of jars appeared totally fine.

Cyan (Bunnell) jar A and jar B. Then just because the lagging bisporus jar.


Inoculated 1/28 and all had a shake at different times.


--------------------
My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

Edited by Kunta Kente (03/02/26 04:17 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk]
    #29504219 -

Thanks for the response man, it was just in time actually, I'd done a little reading and judging by the limited information I found I was thinking it would be a good idea! I was about commit to buying a large quantity!

I'm in Brazil, growing a range of pan strains using an automated martha setup. I've recently moved to another part of the country so unforseen factors including temperature fluctuations are holding me back a bit, I've got some clones I made before I moved and at first I had excellent growth but all the jars stalled out as soon as the weather changed, so I've had to build an incubator. The new jars are 3/4 days in with no signs of growth yet so now I'm worried about the clone LCs....

So anyway, the Brazilian market isn't so good for a lot of things, for instance they don't make mason jars here (at least not anything from a quality glass that would survive the PC, and not with good lids). I had a crack with bags but I work with a glove box and with the size I managed to get hold of working with the bags is really awkward and causes a lot of movement and contacting the upper surface etc (many contam vectors), so I bought a pack of 6 jars on amazon and it cost more than $60 USD!

I use a similar sub mix, but I've never seen anyone mixing manure with coir, and I would definitely give it a try, but as far as I can see coir is yet another product not produced in Brazil (surprisingly), and unfortunately import taxes usually end up being around at least 100% the value of the product + shipping and the only coir available is from the US or China so not really viable. As for straw pellets, I've got no idea what they would even be called here and I highly doubt they would be available... I'll have to stick to straw. I also still need to find a local source of manure which I'm dreading, luckily I still have plenty for several grows so it'll be a while yet.

Thanks again!


--------------------
Taking the 'I' out of 'I AM' since never and a day...

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Shop: Mushroom-Hut Substrate Mix   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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