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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29500946 -

Okie dokie in that case cheers for the heads up as I'm half in that category. Turns out 7 of 8 are good, in the last few hours the pan India has turned from bacterium pooling looking to find strands of hyphae, so no contamination just unfamiliar mycelium on murky agar!
:drunkaf:

Gonna try and find better vessels, better top view.


--------------------
Be lucky, may light walk with you.





Edited by conz0r (02/28/26 09:52 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29501026 -

tree frog said:
It's not quite harmless to humans. They produce a mild toxin that can give some folks intestinal issues like diarrhea.

It's probably fine sprinkled in a casing or substrate.  And it can potentially even survive pasteurization.

But I wouldn't put it in a spray bottle (again) and try to mist with it.  Since that makes it more likely that it will end up on the fruit bodies.



Yeah I guess I should have specified harmless when used as directed. They don’t think you’re going to spray it on something you plan to eat. I really should have remembered that as I too put it in a spray bottle and used it to mist.

I too suffered the consequences.

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Re: Official Copelandia/Panaeolus Thread [Re: RoscoeReturns] * 2
    #29501036 -

I didn't personally suffer the consequences, I heard an anecdote from another TC that sprayed some cubes.  The friend he shared them with called that batch the ass blasters.

Then I did a little research on them, and discovered that they are mildly toxic.  And that the toxin primarily effects the digestive system.  Or more likely, the micro-biome.

This was all around the time I was misting fruits with them last spring trying to control a gnat infestation.

I ended up composting everything that was sprayed :shrug:

But I did get some nice photos first!



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29501319 -

@ tf
Yes I think my MO of taking "just the leading edge" resulting in 3-4mm agar cubes (maybe like 3 of them per inoculation) has resulted in either slow or no growth. Specifically with rye due to its thicker (and surface dry) hulls. So definitely using larger inoculation chunks going forwards.

I still have a ton of rye so I guess I'll try to air dry them slightly less..I do think the main issue is they are simply drier on the surface.

Maybe I'll make a poll thread to see what grain people prefer for pans nowadays. Classically Ive used oats which I feel are very forgiving. Maybe I'll try popcorn like you use since I want to do pans for 8/10 grows.

As an aside, I think the salt content in my C+ test tubs is slowing the myc growth. I have controls and C+ is notably slower in two cube tubs and one ttbvi tub. (20 ml true organic + 20ml jackpot per 8 quart coir). I'll post updates on the CV+ thread if anyone cares~

tree frog said:
But I did get some nice photos first!





Very pretty specimen there!


--------------------
My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

Edited by Kunta Kente (02/28/26 04:10 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente] * 1
    #29501342 -

I've been curious about this too, was corn just a fad?  still a favorite?

Here's some Pan bisporus Coleville on Rye from last year.
   

And here's some on corn from last year.
   

Both grains worked great for me, but grain prep techniques can change a lot, and i take a lot of care in grain prep.

This year I'm trying to compare two different types of corn:  Not because I think rye is inferior, but because I need an excuse to use this corn and I'm glad to have my gallon jars back.

Glass Gems: - a popping corn -  

and
Hopi Blue: - a flour corn -  

So far (its still early)  The only difference I noticed was in grain prep:  The Pop corn started out with a smaller kernel size, but expanded more after cooking, the Flour corn started out with a bigger kernel size, but didn't expand as much.  I assume the flour corn is more nutrient rich, with less of a water content than the popcorn, and the popcorn holds more water.

1 gal of dried pop corn made something like 3 1/2 quart-jars more than the flour corn, but i forgot the numbers of quarts they made, I think it was like 16 qt-jars of flour corn and 19 1/2 quart jars of pop corn - filled to 600mL


^the two gallons of dried grains used.

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Re: Official Copelandia/Panaeolus Thread [Re: Generic] * 1
    #29501445 -

I'm leaning toward it being a fad, that I unfortunately started :lol:

My rye and millet prep worked well for me for gourmets and tamps but not bvi or bisporus.  And when I brought that up in this thread years ago, folks suggested I try AIO which is what I did for a long time.

I tried corn for bulk when pheno was playing with it, and had good results but eventually ran into issues with bacteria so started prepping it dryer.  I also have tried wheat and it's about the same speed as the drier corn prep I use now.

Blue popcorn has antimicrobial properties.  Pans were slow on it for me, but it likely inhibits bacteria too.

One thing I will say about popcorn, is it does have enough nitrogen in it that I can top fruit it and get an average of five good flushes.  Which is about three more than 1:2 popcorn:substrate (no straw in the substrate though).

But I haven't tried top fruiting other grains.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29501559 -

tree frog said:
One thing I will say about popcorn, is it does have enough nitrogen in it that I can top fruit it and get an average of five good flushes.  Which is about three more than 1:2 popcorn:substrate (no straw in the substrate though).




What's your comparative estimated total yield? I would think 1-2 reasonable flushes from a tub would easily outweigh / perform 5 decent flushes from a jar, just based on surface area and more specifically water availability. Not that I have any experience with that...I've only top fruited a few times and that was just because I ran out of tubs / space.


--------------------
My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29501739 -

tree frog said:
I'm leaning toward it being a fad, that I unfortunately started :lol:

My rye and millet prep worked well for me for gourmets and tamps but not bvi or bisporus.  And when I brought that up in this thread years ago, folks suggested I try AIO which is what I did for a long time.

I tried corn for bulk when pheno was playing with it, and had good results but eventually ran into issues with bacteria so started prepping it dryer.  I also have tried wheat and it's about the same speed as the drier corn prep I use now.

Blue popcorn has antimicrobial properties.  Pans were slow on it for me, but it likely inhibits bacteria too.

One thing I will say about popcorn, is it does have enough nitrogen in it that I can top fruit it and get an average of five good flushes.  Which is about three more than 1:2 popcorn:substrate (no straw in the substrate though).

But I haven't tried top fruiting other grains.


hi. Have you tried to feed pans with syringe during fruiting to give more water once it’s excessive amount of them. I am worrying that my little cake will not give them enough to let them mature. Just acting in advance and not sure, they are picky and may abort in my understanding.

Edited by Noah Keeper (02/28/26 09:24 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente] * 1
    #29501756 -

Popcorn




Substrate, probably one four.  Slightly thicker.



I don't know popcorn holds a lot of water. The fruits are denser too.  But I would say that the substrate flushes heavier on the first two flushes then tends to produce smaller fruits.  While the fruit size on the popcorn doesn't really suffer until flush five or six.

But it's expensive to run things just on grain.  I imagine if I used a richer substrate that would make a difference more than the water. This one was on hemp and phase two compost for agaricus.  But it got gnats so I don't know how long it would have flushed.



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29501761 -

You can syringe water the casing, I usually wait until the pins are about an inch tall though.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29501784 -

tree frog said:
You can syringe water the casing, I usually wait until the pins are about an inch tall though.



Thanks 👍🏻🤝 will try to save this culture, too good to be gone 🤞🏻 in a couple days will share results

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29501887 -

If you stop misting once pans are pinning, and you have them in a JCM, does that mean you heavily cut down on the fogging once they pin? Or maybe if there are 3 shelves and fog comes in from the top, you move the pinning ones to the bottom and the slower ones further up top?

Soon I will need to actually dial in my JCM although it's working excellently on my test timers.


--------------------
My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

Edited by Kunta Kente (02/28/26 11:06 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente] * 1
    #29502144 -

Kunta Kente said:
If you stop misting once pans are pinning, and you have them in a JCM, does that mean you heavily cut down on the fogging once they pin? Or maybe if there are 3 shelves and fog comes in from the top, you move the pinning ones to the bottom and the slower ones further up top?

Soon I will need to actually dial in my JCM although it's working excellently on my test timers.


hi. Thanks for replying 👍🏻 it’s  a kind of automated environment box similar with phenodreamer set up, not sure what term is used for it in community. all was done as many times in the past with different varieties of pans, now they are fruiting already so humidifier are keeping environment wet inside. The question popped up cause fruits are dense and my pans tray not really having enough of mass of manure to accommodate so much water to let that amount grow up. My concern was if them stressing after injecting water while fruiting.  In past I tried to feed them with some mixes of LME +water on late stages like once all nutrients gone (after few waves harvested), and there was benefits, but wasn’t try to inject during  fruiting . that was result of feeding, and box already rebuilt, those huge vent  holes gone

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente]
    #29502161 -

Kunta Kente said:
If you stop misting once pans are pinning, and you have them in a JCM, does that mean you heavily cut down on the fogging once they pin? Or maybe if there are 3 shelves and fog comes in from the top, you move the pinning ones to the bottom and the slower ones further up top?

Soon I will need to actually dial in my JCM although it's working excellently on my test timers.



By the way, once I’ve seen those amazing Fahster ziplocks method, built my own modified with simple jar humidifier and more holes all around for tamps and ingeli😁


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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy] * 1
    #29502304 -

Mr Piggy said:
tree frog said:
Oh and you don't have to put them in the fruiting chamber.  I'm paranoid about gnats atm since I moved everything onto the same level of the house my partner has all her plants.

I used to do this on a shelf instead.




Preemptively sprinkle mosquito bits (the kind with bti) in all of the pots.  I collect house plants like they will fix my mental health and I have to drop bits in each one to prevent gnats.  It works on the gnats, but aside from a lil dopamine bump each time I get one, the plants haven’t fixed me yet.



It kinda works but it's not a miracle cure. I've once got so mad at the fuckin gnats that I've covered the entire surface of the sub with a thick layer of BTI. They died off but later came back with vengeance. It was horrible. They might have developed a resistance or the BTI became ineffective, idk.

The ideal solution would be predatory nematodes, but these are 1000x harder to keep than some powdered bacterial spores that you can just forget about for a decade.

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29502421 -

I hate gnats so much. I think I'd rather shit my pants every time I trip than deal with these fuckers ever again


--------------------
what i've been growing

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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #29502546 -

Adas said:
Mr Piggy said:
tree frog said:
Oh and you don't have to put them in the fruiting chamber.  I'm paranoid about gnats atm since I moved everything onto the same level of the house my partner has all her plants.

I used to do this on a shelf instead.




Preemptively sprinkle mosquito bits (the kind with bti) in all of the pots.  I collect house plants like they will fix my mental health and I have to drop bits in each one to prevent gnats.  It works on the gnats, but aside from a lil dopamine bump each time I get one, the plants haven’t fixed me yet.



It kinda works but it's not a miracle cure. I've once got so mad at the fuckin gnats that I've covered the entire surface of the sub with a thick layer of BTI. They died off but later came back with vengeance. It was horrible. They might have developed a resistance or the BTI became ineffective, idk.

The ideal solution would be predatory nematodes, but these are 1000x harder to keep than some powdered bacterial spores that you can just forget about for a decade.



I don't use bits in mush cult, I use them to prevent a population from establishing in my house plants.  For that use they have so far been 100% effective.  I eliminated a population of thousands, THOUSANDS of them from my house.  It was a nightmare for a month, but now I have complete control of the population.  The only gnats we see are the ones that come in from outside.  I think mush cult doesn't have long enough of a timeline for BTI to work, it took a month and a half of repeated treatments before I got rid of them.  I had to soak bits in a gallon jug of well water and water the plants with it about once every week to two weeks, each watering making a new batch.  I was honestly shocked by the results and have kept up on it since :thumbup:  My poor carnivorous plants aren't getting enough to eat.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Official Copelandia/Panaeolus Thread [Re: Mr Piggy]
    #29502622 -

Quick question: is there a consensus on how well pans on agar tolerate cold storage for a couple of weeks? I've started too many plates and need to press pause for a bit until I get more organised...are they going to be OK in the fridge for a couple of weeks?

I see different opinions in this thread over the years.

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Re: Official Copelandia/Panaeolus Thread [Re: bongoman]
    #29502628 -

I keep my mini-fridge at 50f and they continue to colonize, but very slow.

How colonized are your plates currently? And how long until you have to go?

Because fully colonized plates generally can be dicey in regard to recovery in my experience.  But if you have a culture that has some room to grow, I bet they would do fine in the fridge for a few weeks.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29502635 -

tree frog said:
How colonized are your plates currently? And how long until you have to go?





They are all pretty grown out, mostly T0 and T1 plates — would it be worth just doing seem fresh transfers instead to buy me some time?

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