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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Copelandia/Panaeolus Thread [Re: stagger] * 1
    #29434676 -

I received a TTBVI print for the first time since '21. It was an extremely small thing, but for those of us who know, only a couple spores is needed.. I think my biggest flordia redspore was the size of a quarter. I'm sure I'll be impressed with the genetics, regardless of the size of fruits.


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In this life, trust is a luxury we cannot afford.

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead] * 1
    #29434859 -

hardhead43 said:
Gilmour Hendrix said:
I knocked up two jars with a bvi plate I have had in storage since October. These were done Sunday evening. I'm comparing to faht's speedy quick jars he shared. These are very slow in comparison.

I have questions.

Should I go back to spore?

Do I need better genetics?

Did I let the plate sit too long?



The condensation in the jar is likely because of the sun coming through the window to warm the jars during the day, then cooling at night.


I've had some slow jars that did ok, but that looks very sketchy.  Too wet looking to me.  Lots of burst grains.  TTBVI does great on millet and millet is so stinking easy.  Even WBS that is mostly white millet.....1 cup of dry grain, 1/2 cup water, pinch of gypsum helps with clumping.  Just dump in quart jar and straight to PC.  No prep no nothing.  2.5hrs at 15 psi and then shake a couple times (as soon as pressure drops; and once again when still warm).  TTBVI does fine on this and the larger # of inoculation points is  plus for colonization speed.


Thanks, hoss. It took a little while for the myc to take off. I shook the jar last night since I could see growth. Today, the jars are gaining momentum. 24 hr recovery looks great!. I'll let these ride but I am also going to start from scratch.

Thank you for the advice!


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Re: Official Copelandia/Panaeolus Thread [Re: Gilmour Hendrix] * 8
    #29434884 -

Had a bad year, getting back in to the swing of things!

Got MIB redspore germination and a few other pan projects too.

Cambo x bvi is taking off!

Really struggling financially but I'm going to set a side a few $ to spread the love in January! ☺️☺️


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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 1
    #29434906 -

fahtster said:
Ty.  As you probably know I’ve been doing dry grains for cubes for a long time but Baba Yaga did it with pans 5 years ago.  I was like you, I thought there’s no way with how delicate pan myc is that it would revive.  Turns out it’s the fastest species to revive.  lol  whoda thought it

These grains were all dried out in July and this is only 2 DAYS on water agar



That’s amazing man! Thank you for sharing!

I wonder how long they can stay viable if I store them in the fridge.


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Ps. Serbica official thread - serbica connoisseur :mushroom2: :smirk:
📨 Journey of a Letter ✉️ the thread about how letters travel, how to address an envelope, and how to expedite delivery 📨📭
☞ Guide to the Shroomery - how to grow shrooms from A to Z guide ☜
If you want to dive in and grow some Pans, this is the place to start! ⥾
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Official Copelandia/Panaeolus Thread [Re: thirdeyewild]
    #29434907 -

im not sure if you can see this very well but at the top is grain that looks concerning I finally did a break and shake. It looks blue green mixed and the mycelium doesn't look super healthy. I read this is normal after a break and shake but wanted to confirm cause it looks nasty also it had a ton of grains stuck to the agar and the mycelium was so light it was a lot bigger amount of grain it was touching than I could see. A huge clump of it was all stuck together I spread it out probably about 10-15% of the jar was stuck to the agar before this.


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I am the master of my fate,
      I am the captain of my soul.

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Re: Official Copelandia/Panaeolus Thread [Re: CaptainTrips420] * 13
    #29435687 -

I would like to thank everyone who has filled this forum with useful information and contributions. I am currently very happy with my harvest.


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Re: Official Copelandia/Panaeolus Thread [Re: blueprint]
    #29435690 -

:grannycheer:

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Re: Official Copelandia/Panaeolus Thread [Re: blueprint]
    #29435697 -

blueprint said:
I would like to thank everyone who has filled this forum with useful information and contributions. I am currently very happy with my harvest.



:chefskiss:


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk]
    #29435707 -

Another question just occurred to me. I have now received a spore print from Pan Cyan' “British Virgin Islands.” Does it matter whether I make a spore suspension and inoculate directly or go the agar route?Or is liquid mycelium better?


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Edited by blueprint (12/21/25 04:04 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: blueprint] * 1
    #29435712 -

blueprint said:
Another question just occurred to me. I have now received a spore print from Pan Cyan' “British Virgin Islands.” Does it matter whether I make a spore suspension and inoculate directly or go the agar route?


Yes, always agar first :awesomenod:


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: blueprint] * 2
    #29435713 -

blueprint said:
I would like to thank everyone who has filled this forum with useful information and contributions. I am currently very happy with my harvest.




:vibin::raisemyglass::vibin:


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What are they saying? Listen carefully, it might be something you need to hear...




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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk]
    #29435714 -

Kloakk said:

Yes, always agar first :awesomenod:


Why is it always better to use agar, or what is the disadvantage of using a syringe or liquid mycelium?


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Re: Official Copelandia/Panaeolus Thread [Re: blueprint] * 1
    #29435717 -

Germinating spores on agar will enable you to identify any contamination and isolate healthy mycelium from the contaminants. That is not possible with germination in a liquid.

The additional advantage of starting with agar is that it enables you to isolate sectors of mycelium that appear to have desirable characteristics such as growing fast or showing early emergence of primordia. Again this isolation of sectors is not possible when you germinate in a nutrient solution.

I’m sure others will chime in with other reasons …


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What are they saying? Listen carefully, it might be something you need to hear...




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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #29435736 -

PsiloPsychIn said:
Germinating spores on agar will enable you to identify any contamination and isolate healthy mycelium from the contaminants. That is not possible with germination in a liquid.

The additional advantage of starting with agar is that it enables you to isolate sectors of mycelium that appear to have desirable characteristics such as growing fast or showing early emergence of primordia. Again this isolation of sectors is not possible when you germinate in a nutrient solution.

I’m sure others will chime in with other reasons …


:whathesaid:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #29435892 -

PsiloPsychIn said:
Germinating spores on agar will enable you to identify any contamination and isolate healthy mycelium from the contaminants. That is not possible with germination in a liquid.

The additional advantage of starting with agar is that it enables you to isolate sectors of mycelium that appear to have desirable characteristics such as growing fast or showing early emergence of primordia. Again this isolation of sectors is not possible when you germinate in a nutrient solution.

I’m sure others will chime in with other reasons …


I guess not other reasons but to expand on your reasons.

Bacteria thrives in liquid.  Inoculating from a wedge, if you get a little bacteria in a jar you might still get a very successful grow (or might not, depending a lot on how wet your grains are and how much bacteria went into the jar).

If you make an LC with a little bacteria, or squirt spores into a jar with a little bacteria and a little water, you're going to end up with a lot of bacteria.

Personally, I don't mess with liquid anything aside from culture storage.  And I'm trying to move away from that toward dry grains and water agar plates.  But, when I do revive something from water stasis, it goes right into a cabin.


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 7
    #29435976 -

Fruiting something new :smile:

https://www.inaturalist.org/observations/177908874



And I have a feeling this one might be conspecific with the European bisporus judging by those in situ inNat photos.  The species we have in America might be something closely related but separate.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (12/21/25 12:37 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29437811 -

Hi. Very interesting method to save culture 👍🏻 would you mind to share how exactly it’s goes? You let mycelium colonize grain and dry it? How do you dry that grain? Thanks 🤝
fahtster said:
Ty.  As you probably know I’ve been doing dry grains for cubes for a long time but Baba Yaga did it with pans 5 years ago.  I was like you, I thought there’s no way with how delicate pan myc is that it would revive.  Turns out it’s the fastest species to revive.  lol  whoda thought it

These grains were all dried out in July and this is only 2 DAYS on water agar



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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper] * 1
    #29437993 -

I use These lids but you can simply leave a small amount of grains in your jars after you spawn them.  You want to keep them sterile so being quick with putting the lid back on is crucial or doing it in a SAB or in front of a hood/ffu.  Spawn jar lids have a small GE filter so a small amount of kernels will dry out fastest. 

You can put a pile in a sterile Petri dish uncovered in front of the ffu.  This is the fastest but most risky as it takes about a day and you have to leave your hood running for that long

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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #29438081 -

Going to be trying to grow some pans for the first time after running bags with needle 2 grain, cubes for years using a premade substrate. My method of growin’ shrooms thusfar has been extremely rudimentary and friends love them, but I feel like I’m not even trying and they are subpar compared to what I could be doing. I will most likely attempt to make my own agar with oats (anyone got a good tek?)

I will be attempting a Martha tent. I want to master this and am willing to give it a couple tries if it doesn’t work right away. I plan on using peace river. Does anyone have any suggestions for using these particular genetics? I understand the different genetics have variables on what kind of medium for agar and substrate. Any suggestions for a novice grower trying to step it up ?


Last thing, a very novice question. Will a Martha tent have a higher potency than a gordotek tub? I’m assuming it will. Would it be a higher potency for cubes as well? Can I attempt cubes and pans in the same tent?

Edited by Dj_karma_magnet (12/24/25 01:19 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Dj_karma_magnet] * 4
    #29438113 -

For tents, look into jakeoncid and Asura

Jake’s
https://www.shroomery.org/forums/showflat.php/Number/25925194#25925194

Asura’s
https://www.shroomery.org/forums/showflat.php/Number/25987049#25987049

Tree frog has a great thread on pans in general
https://www.shroomery.org/forums/showflat.php/Number/29144450#29144450

Genetics are going to determine potency, not the chamber they’re grown in. 

If you don’t want to go the whole tent route, there’s also the jcm chamber
https://www.shroomery.org/forums/showflat.php/Number/26565367#26565367

Edit: I always forget about the dreamer tub, sorry pheno:
https://www.shroomery.org/forums/showflat.php/Number/29041297#29041297

Cubes and pans have different growth parameters.  You can grow cubes in a tent set up but it’s unnecessary.  You can grow cubes in a pan environment but you “can’t” grow pans (well) in a cube environment.  Pans require higher fae with periodic rh drops for evaporative cycles to grow optimally.  Cubes can grow great in set and forget tubs with proper passive fae and high rh

Edited by fahtster (12/24/25 02:24 AM)

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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