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Re: Official Copelandia/Panaeolus Thread [Re: yearofnolight] * 12
    #29357683 -

MIB McRiB 😹 grain, composted manure, coir, gypsym, vermiculite. Cased with CVG.



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Nature Reclaims All.

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Re: Official Copelandia/Panaeolus Thread [Re: Wedgie] * 1
    #29357686 -

Great job wedgie, nice to see you pull that off


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In this life, trust is a luxury we cannot afford.

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Re: Official Copelandia/Panaeolus Thread [Re: CrimsonRambeler] * 5
    #29358057 -

Just seeing the first tiny baby pins on these trays of Fire Dancer. These were put straight to fruiting conditions in a tub with fan/fogger after spawning to bulk. Low 80sF and 94-99% humidity maintained for 6 days. Substrate is coir/verm/bagged manure




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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29358129 -

Maormuisir said:
Were there any special features about these beautiful friends? It is said that they are quite simple, but love warmth? How was the humidity regulated? A great result!!!



fahtster said:
Third flush weza shorties





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Go deeper and take more!!!

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Re: Official Copelandia/Panaeolus Thread [Re: Maormuisir] * 4
    #29358133 -

I used a Jcm chamber and temps were 78F

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 1
    #29358146 -

fahtster said:
Weza finishing out its second flush..


I love days when all the trays in a tub are doing something I can stare at lol




Ohhh love they so mutch


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Go deeper and take more!!!

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29358147 -

:heart::thumbup:


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Go deeper and take more!!!

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Re: Official Copelandia/Panaeolus Thread [Re: Maormuisir] * 8
    #29358349 -



4:2:1:1 peat:limestone:worm casting:gypsum casing.  Fully colonized.  Pinned with the lid flipped to keep humidity high and not have to mist.


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29358366 -

Why am I getting all these stalk-like vertical overlay in my cyan trays? Photos show the trays after 3 days in a Martha tent on JOC cycle. Sub is black kow/ coir, casing is peat/verm, following the AIO jar tek.

Is the overlay concerning?

Edited by philmyco (09/28/25 01:32 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: philmyco] * 2
    #29358381 -

It's not overlay.

Quote:
When a colonized casing dries out, it can no longer form hyphael knots.  And without knots, it won't pin.

A colonized casing alone isn't overlay.  And there are various strategies to avoid it.

Such as, isolating wispier mycelium on plates, initiating pinning prior to the casing colonizing, and keeping the rH high enough that the casing doesn't dry out during initial first flush knot and pin formation.



As to why it's doing it, I don't know.  I only saw that type of growth once when I tried to use coir and calcium to case Mystery SA.  It still pinned okay, but the casing just wasn't a good casing for the project, and peat based casing did better.

It could be other factors causing it, like bacteria or a mold hitchhiking.  But what you're seeing is a form of thick aerial mycelium.  As long as it doesn't dry out and prevent pinning, it's not overlay.  And even if the bit above the casing dries out, you have a lot of other pinning surface.

This wasn't even overlay, because I kept the conditions very humid so it could pin.



See, they pushed right through the mat, because it wasn't dry.



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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29358391 -

All of you peps have amazing grows.

It's been just over a month since I was generously gifted my pan prints. I have LI's working with MIB and TTBVI and several grown out agar plates currently hibernating in the fridge. I've been using 1/2 pints of rye berries as agar and LI tester jars and now finally have a bvi colonizing on cow manure compost, straw and cvg. I'm going to prep and pc* my peat, verm, calcium carbonate casing today. Fingers crossed. I have another few test jars to birth this week. I'm so excited, but I'm still trying to dial iny dreamers. I'm switching from a digital fogger to a manual one that I can run on a cycle timer. The digital one with the hydrostat doesn't seem accurate enough. The fogger says it's 90%+ RH, but my several gauges say 85%.

Sorry for the crap pic, it's humid in there but the myc seems to be colonizing the sub. It's only a few days in and I didn't want to open it yet.


*Edit PC not pasteurize


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Edited by Centerfinger (09/28/25 02:35 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29358430 -

Thanks tree. This post from hindsight adds some additional clarity as well https://www.shroomery.org/forums/showflat.php/Number/28164322#28164322

I’m running a JOC cycling tent but it’s only two shelves (the mini greenhouse) so I expect there’s just too much humidity. I’ll cycle it down a bit and see how the next tray does

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Re: Official Copelandia/Panaeolus Thread [Re: philmyco] * 2
    #29358463 -

If the aerial myc dries out, it's probably worse for pinning.

Culture selection can help too.  Wispy growth on plates tends to do better than puffy growth, for example.



Wild bvi germinated on water agar, t1 to 0.5% LME.  Once it grows out a little bit more I will transfer from the leading hyphae (before it reaches other colonies because I'm also looking for monos).  Goal being organized wispy growth like this on a master plate for grain inoculation.


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29358496 -

I have bvi spore plates (2% MEA) going now. I was concerned with the whispy growth but after reading your post I assume that is normal?


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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29358508 -

Maybe it’s the premade plates but I never got whispy despite multiple germ plates from spores and transfers.  Thinned out but pretty yomentose still.

I just made some rye water plates though so that might help for the next run

Edited by philmyco (09/28/25 05:30 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Gilmour Hendrix] * 2
    #29358712 -

Pan cyan mycelium varies from extremely tomatose, to very wispy with clearly visible leading hyphae.  The leading hyphae help to discriminate between mold and mycelium and is a good place to take transfers from, especially if it's organized.  The tomatose growth has a tendency to make pseudo sclerotia on plates, or fluffy aerial mycelium if the conditions aren't great in the casing.  If the fluffy mycelium dries, the colony will have a hard time fruiting.  I.e. overlay.

I'd suggest diluting grain water by fifty percent or more phil.

Anyway, this is a good image of the type of growth to look for taken from baba's pans in monos thread.  Note this is pan cyans on low nutrition agar (probably 0.5% or 1% LME).  Other species can behave differently on a plate.

https://files.shroomery.org/files/22-42/629665002-P1040435.jpg

Thread for reference.  There is a lot of good agar advice here.

https://www.shroomery.org/forums/showflat.php/Number/27637997/page/1


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29359340 -

looking through those threads. I have a moldy Pan Cyan Peace river trying to clean. Hoping to have it clean. Started some on plates and BRF pucks. I still stuggle in noticing hidden mold on plates.

Has anyone had invitro pan pins on the brf or brf/poo pucks?

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Re: Official Copelandia/Panaeolus Thread [Re: fatpup]
    #29359463 -

Yep. BRF and ground millet pucks and cakes have fruited for me. I haven’t added any fertilizers yet. That’s a fall project.

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Re: Official Copelandia/Panaeolus Thread [Re: The Tao] * 8
    #29359554 -



MIB @ 30hrs.  Wish me luck


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MUSHROOMS RULE

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Re: Official Copelandia/Panaeolus Thread [Re: The Tao]
    #29359616 -

The Tao said:
Yep. BRF and ground millet pucks and cakes have fruited for me. I haven’t added any fertilizers yet. That’s a fall project.



Did you do anything to get them to pin or just they pinned in the jars?

I did spore print>agar>brf>agar. Just put the cleanest to some bottles to hopefully fruit. BRF pucks at 2 months and no sign of pins. They dont look great though. Plate look much better. But its been 5+ transfers.

But thank you for the info.
shroomery

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Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags


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