Gilmour Hendrix said:

I am using dry lme. This time I used an electric kettle and a glass measuring cup. Poured the boiling water a little at a time over the ingredients all while whisking the tits off of it. Simmering might make it a little better but still have specks. I'll try PDA and see if I have the same issue. Thanks for the tip!

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Kloakk said:

sporethemedusrname said:

hi, im new to all this, do these like, look healthy?

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Welcome, happy to see you here Great to see you're doing agar from the get go.
Might just be the pictures, but it looks like there is some yellow/brown color on the transfer pieces. Could you take pictures of the underside of the plates?

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aw, ty for the welcome! in fairness i did do a batch of PF tek last year to see if i had a taste for it lol, but now im starting with learning agar

i took some pictures from the back but i couldn't get anything very clear through the agar x.x

sporethemedusrname said:

aw, ty for the welcome! in fairness i did do a batch of PF tek last year to see if i had a taste for it lol, but now im starting with learning agar

i took some pictures from the back but i couldn't get anything very clear through the agar x.x

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That's awesome, I'll have to do some cakes eventually, just not got around to it yet

And the pictures are okay, if you get brighter back light you might get even better view of what's going on. What temperature are the plates sitting at? I ask because browning from the center could be several things, I've notice that heat stress and bacteria can do this, I'm sure there are other reasons I've not yet encountered as well

I suggest doing a couple more transfers and if you feel up to it, put one transfer of each culture through a cabin or a trench.

Kloakk said:

That's awesome, I'll have to do some cakes eventually, just not got around to it yet

And the pictures are okay, if you get brighter back light you might get even better view of what's going on. What temperature are the plates sitting at? I ask because browning from the center could be several things, I've notice that heat stress and bacteria can do this, I'm sure there are other reasons I've not yet encountered as well

I suggest doing a couple more transfers and if you feel up to it, put one transfer of each culture through a cabin or a trench.

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i took a transfer from each plate like you suggested.

these plates should be living in like the high 60 to mid 70s, but they are near a window, so they might get warmer than i realized even if they're not getting be direct sunlight. the wedges that are the actual discolored part are from a batch of agar that accidentally came out weird, so maybe that part of it?

i tried doing a cabin on a different culture earlier that has some kind of yellow slime contamination, but i didn't realize i should store it on its side and it flooded and i worry that everyone drowned or the slime got out with the flood waters :/ live and learn i guess lol

sporethemedusrname said:

i took a transfer from each plate like you suggested.

these plates should be living in like the high 60 to mid 70s, but they are near a window, so they might get warmer than i realized even if they're not getting be direct sunlight. the wedges that are the actual discolored part are from a batch of agar that accidentally came out weird, so maybe that part of it?

i tried doing a cabin on a different culture earlier that has some kind of yellow slime contamination, but i didn't realize i should store it on its side and it flooded and i worry that everyone drowned or the slime got out with the flood waters :/ live and learn i guess lol

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Temperature should be fine. I suppose it could be the agar, but I wouldn't bet on it, most likely stressed out mycelium that can be caused by different thing, but don't waste time on trying to find out, just yet.

See how these new transfers play out first I would not have my cabin plates stores on it's side, that would just help bacteria get out of the cabin. And a little water pooling inside the cabin is normal for me as well, and that is another good reason to not store it sideways.

Storing plates on it's side has it's value because bacteria can't climb, but mycelium can, kind of counter productive with a cabin plate though. If you already have a normal plate with bacteria you could store the plate standing and the mycelium might climb high enough, away from the bacteria to get a clean transfer

And it's not that your plates looked all that bad anyway, so maybe this transfer will do the trick, let us know how it turns out

divineverdance said:

tryin to  figure this agar stuff out , anyone help me understand? I'm retarded,,,

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sloug said:

looks great so far. just let it grow out a bit more and you will be able to tell better if it needs another transfer. but it never hurts to go to t2 or 3 especially if ur new to agar/cloning. there are so many factors that to take into account for growth rate but 7 days for that amount is not uncommon at all.

great work!

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Kloakk said:

Temperature should be fine. I suppose it could be the agar, but I wouldn't bet on it, most likely stressed out mycelium that can be caused by different thing, but don't waste time on trying to find out, just yet.

See how these new transfers play out first I would not have my cabin plates stores on it's side, that would just help bacteria get out of the cabin. And a little water pooling inside the cabin is normal for me as well, and that is another good reason to not store it sideways.

Storing plates on it's side has it's value because bacteria can't climb, but mycelium can, kind of counter productive with a cabin plate though. If you already have a normal plate with bacteria you could store the plate standing and the mycelium might climb high enough, away from the bacteria to get a clean transfer

And it's not that your plates looked all that bad anyway, so maybe this transfer will do the trick, let us know how it turns out

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lostsoul1976 said:

Pan Peace River clone taken on 9/1.  I know it’s early but how’s it looking? Haven’t had any success  with Pans on agar before. I made a batch of water agar cause I think I read somewhere it’s good for clones. The fruit I cloned set in the fridge for 2 days before I could pour some plates. It’s only been 2 days, is this amount of growth normal?




Took the pics from the bottom, lid was a little foggy 😋

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sporethemedusrname said:

:o i've just been reflexively tilting all my wet plates worrying about standing water, ty for explaining all the subtleties about sideways plates and cabins!

i did that cabin mostly just for practice and cause i thought it sounded cool when i read about it and i got excited lol. so i wasn't really expecting it to work on this try and i kinda freaked out when it filled it water and i assumed it was toast. i'll definitely try putting what you explained about cabins into action next time i take transfers from my gross slimy cleaning practice culture!

i'm about to be out of town for a few days, so i'll see how everything has grown once i get back. ty again for explaining all this stuff to me!

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Yeah, I did a lot of cabins before I felt comfortable with it, practice makes masters so if you feel like doing more cabins go for it.

One thing about cabins and condensation/water inside the cabin. It took me a couple of looks before I realized that what I thought was a flooded cabin with just a little air pocket is actually the opposite, it's a little water inside the cabin, the same goes for your plate, just wanted to mention in case you didn't see it yourself, so your plate is not flooded at all.