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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] * 2
    #29291991 -

PsiloPsychIn said:
Hi all you Pantastic People!
I wrote a note and posted it in mush cult pic of the day (I think forum) thee are t explained I kind of had a melt down about 2 years ago and just suddenly stopped all cultivation activity.
Turned my back on all my jars, plates, bags trays, etc. I only bothered to throw some things away if they got too stanky or pests were becoming obvious. In that 2, 2 plus years I pretty much Bebe logged into the Shroomery. If I was involved in a trade with you… I apologize. Please remind me and I’ll make it right! I gave away a ton of prints back in the day (including pans - so maybe that will help).

About a month ago (a month after I retired from my day job), I started back into mush cult again.
First it was dealing with the mess / aftermath.  Lots of old plates, rotten or dedicated jars etc.

Here is a mind blowing thing I wanted to share with you.

You may recall that I spread around a LOT of Bunnell spore prints - the first batch came from a generous member who could not afford the postage but sent me a couple dozen with the understanding that I’d pass them on.  Then when I started growing my own, I printed bunches of them and gave them away.

I was working with a good culture before the melt down. It was fast and tended to cluster. It also tended to produce something like sclerotia on older plates:




Well almost TWO years later I opened that very plate and took transfer material from several places (including that blue warty thing) and here is the T1 “rescue” plate:





Hey Psilo, it's awesome to still see people working with the culture! How did you store the plate that you were able to revive the myc 2 years later?

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 4
    #29292689 -

What’s Up Adas!
That certain member who sent me a bunch of Bunnell prints to redistribute to others was you - wasn’t it?  I was going to search my inbox to find the correspondence - but I for got how to.

As for the storage - pretty rudimentary, not intentional. It was a pretty thick pour of LMEA and quite tightly wrapped in parafilm. Then just stored in my lab room at room temperature (with some daily variations due to a grow light in the room).  It may have also been enclosed in a zip lock with some other abandoned plates. The part transferred from sclerotia thing came alive first after transferring - but as you can see all parts except that one on the left came back. A real.

Testament to how strong and resilient that Bunnell is. The Force is Strong with this One! I saw that a vendor did a cross between Bunnell and Snake Gully - I ordered some spores and hope to get that going in the next few months.

Thanks for the welcome back Asura, Adas, &  Chris77.

Thank you for all that info on the cinctulus Crimsom Rambler! Very interesting!

I never tried to fruit my Bunnell bumps and I didn’t look for them in substrate… only saw them form on old plates…



--------------------
What are they saying? Listen carefully, it might be something you need to hear...




Edited by PsiloPsychIn (07/23/25 10:56 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] * 3
    #29292873 -

My AIO bispo jars are almost ready to spawn! As I've seen people mention that they need a lot of water, how much is that really? Like do I soak the living shit out of it and let it float when I spawn it and then mist more than you'd do with Cyans?

I like the look of these jars way more than my last failed marshmallow TTBVI. Also it's my first time being able to do a fully clean grow and not cutting any corners (except the micropore tape instead of a proper filter) so hurray!:dancer: I don't know if I fucked up yet but I did everything according to the book so I guess time will tell but I've got a good feeling of this batch!:rockon:


--------------------

Edited by SkanskKorv (07/24/25 06:05 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 4
    #29292962 -

I mostly grow them like cyans just at a bit cooler temperatures and with more patience.  The cooler temps are naturally wetter, less evaporation and the air holds less water.  The tubs are the same aside from temp and the natural affect that has on water cycles.




When the pins do come in, I like to give the casing a good mist to support the pin set.  But only once the pins are set, otherwise just mist to replace evaporation if you need to.

Like dew rather than mud.



This one is in the heated tubs as a test, and I've had trouble keeping the casing right.  Though it did pin already I imagine this will affect the pinset and I'll need to rerun this sample with better conditions.  Maybe a thinner casing would do better in the warmth, harder to get water logged.  In the room temperature tubs (74f) I tend to go with 1/4" and give them a few days to colonize it.



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (07/24/25 09:40 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29292976 -

Thanks once again!

How much is too much when it comes to the temp for bispos? I'm having issues keeping it below 77f during the day in my grow room at the moment and sometimes it's even a bit higher. I guess I'm gonna have to make a really thin casing then...


--------------------

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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 1
    #29292994 -

77f is fine, thin casings are hard to water log and easier to work with as you approach 80f.  Basically run them like cyans until pins set, then give them a good misting.

Outdoors they aborted quickly past 80.  But they also had wind, sun, and lower humidity to contend with.

It's less about the higher temperature in other words and more about how that impacts humidity and evaporation cycles.

Anyway, patience is the biggest difference.  They're still very close to cyans. Just slower and prefer subtropical rather than tropical temps.  And much less likely to abort from excess water.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29293027 -

The issue is that I'm still not 100% sure about Cyans either since I've only made two attempts with them and the first was meh with a few fruits whilst the second sucked balls and just gave me overlay as you probably remember:crazyeyes:

I don't think my fail was due to the moisture but I'm still unsure how much water to give it at spawn. The only method I really know well is the pf tek and you dunk those cakes to completely soak them for 24h so do I try to make the casing as wet as possible after applying it by misting it like crazy or what indicates that it has enough moisture to produce enough shrooms?

I'm having a hard time telling if the picture after you misted the casing in your AIO guide is completely soaked or just a bit damp and I wanna keep doing everything by the book since I'm finally on a good track now:rockon:


--------------------

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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 3
    #29293070 -

I run everything around field capacity and don't mist my casings after application anymore.  But may when I put it into fruiting if I case heavier and it looks like it needs it (only bispo and close relatives anymore, but may move to thin casings on them too).

Anyway, tropical and subtropical pans like condensation and evaporation.  These are two competing factors, as very high humidity causes condensation rather than evaporation, and too much evaporation will dry out the fruits.

This means there's no sweet spot.  But rather, you want alternating periods of condensation (94+ rH) followed by evaporation (exhaust fans and humidity dips under 80 rH).  The evaporation will slowly raise the humidity up after the exhaust cycles, the foggers on a timer push it up further, fans exhaust again.  Cycle rests for ten minutes.  Rinse, repeat.

It's not the only way.  But, it helps a lot with good morphology.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
    #29293127 -

PsiloPsychIn said:
What’s Up Adas!
That certain member who sent me a bunch of Bunnell prints to redistribute to others was you - wasn’t it?  I was going to search my inbox to find the correspondence - but I for got how to.

As for the storage - pretty rudimentary, not intentional. It was a pretty thick pour of LMEA and quite tightly wrapped in parafilm. Then just stored in my lab room at room temperature (with some daily variations due to a grow light in the room).  It may have also been enclosed in a zip lock with some other abandoned plates. The part transferred from sclerotia thing came alive first after transferring - but as you can see all parts except that one on the left came back. A real.

Testament to how strong and resilient that Bunnell is. The Force is Strong with this One! I saw that a vendor did a cross between Bunnell and Snake Gully - I ordered some spores and hope to get that going in the next few months.

Thanks for the welcome back Asura, Adas, &  Chris77.

Thank you for all that info on the cinctulus Crimsom Rambler! Very interesting!

I never tried to fruit my Bunnell bumps and I didn’t look for them in substrate… only saw them form on old plates…





It was me indeed! Glad I sent you all those prints before the culture completely crapped out on me. Now I'm enjoying the slow and predictable Bisporus :lol:

It's absolutely insane that you just stored it on a plate and it survived. So I guess that rules out the hypothesis that it's low osmotic pressure that preserves cultures in the CryoVial TEK storage method.

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29293128 -

reading another mighty fine post of yours i cannot help myself but have to praise you, tree frog. you are truly precious. thank you


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

Edited by chris77 (07/24/25 12:30 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29293132 -

tree frog said:
I run everything around field capacity and don't mist my casings after application anymore.  But may when I put it into fruiting if I case heavier and it looks like it needs it (only bispo and close relatives anymore, but may move to thin casings on them too).

Anyway, tropical and subtropical pans like condensation and evaporation.  These are two competing factors, as very high humidity causes condensation rather than evaporation, and too much evaporation will dry out the fruits.

This means there's no sweet spot.  But rather, you want alternating periods of condensation (94+ rH) followed by evaporation (exhaust fans and humidity dips under 80 rH).  The evaporation will slowly raise the humidity up after the exhaust cycles, the foggers on a timer push it up further, fans exhaust again.  Cycle rests for ten minutes.  Rinse, repeat.

It's not the only way.  But, it helps a lot with good morphology.




Isn't the casing application and fruiting at the same time though? In the guide you wrote that you put them straight into fruiting conditions after applying and misting the casing or has this been changed so that I need to let it colonize at field capacity and then put it in fruiting conditions and give it a heavy mist when it's ready?


--------------------

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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 8
    #29293213 -

While not every single cap dropped a print, a bunch of them did!

Thank you TF for suggesting I increase the airflow in my JCM to get more developed caps!




--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] * 10
    #29293323 -

Hello Brother! I'm glad to see you again too. I remembered you fondly,  grew your Florida Bunnell.:mushroom2::super:

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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 1
    #29293450 -

Essentially, if I use a very thin casing, I apply and put it into fruiting.  Rather or not I mist depends on how dry the colonized substrate is.

If I use a thicker casing, I let it colonize before fruiting.  And generally give a light mist when I do.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 4
    #29293594 -

Myc check please

3 TTBVI germ plates




We looking good? Never worked with pan myc before

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Re: Official Copelandia/Panaeolus Thread [Re: tholos]
    #29293612 -

Looks good!


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29293835 -

tree frog said:
Essentially, if I use a very thin casing, I apply and put it into fruiting.  Rather or not I mist depends on how dry the colonized substrate is.

If I use a thicker casing, I let it colonize before fruiting.  And generally give a light mist when I do.



Alrighty then. Thank you for answering my silly questions.:rockon:


--------------------

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Re: Official Copelandia/Panaeolus Thread [Re: SkanskKorv] * 11
    #29294435 -

Holy fuck. I FAFO and took too many pans. I spent a couple hours begging for it to end. I wanted to scream, but my son was in the next room over and I didn't want to traumatize him. I will not be doing that again.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon]
    #29294471 -

Oh shit how much?

Edit:  And feel free to share more info in the definitely not official fafo thread.

Sorry I'm trying not to laugh, I'm glad you're alright fam :smile:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (07/25/25 10:24 PM)

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon]
    #29294474 -

Yeah...those are scary. Welcome back to here.


--------------------
Cultivating Panaeolus cyanescens


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Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale


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